Dr Sarah R Hart

Title: Lecturer in Bioscience
Phone: +44 (0)1782 733759 or 734639
Email: s.r.hart@keele.ac.uk
Location: Institute for Science & Technology in Medicine, Keele University
Offices: School of Medicine 1.03, or Huxley Building 303B,
Keele University, Staffordshire ST5 5BG, United Kingdom
Lab: Harvey Laboratory 213, Huxley Building, Keele University, Staffordshire ST5 5BG, United Kingdom
Role: ISTM Research theme: Healthcare Technologies
Contacting me: By phone or e-mail
Dr Sarah Hart

I came to Keele with an EPSRC International Life Science Interface Fellowship, worth £336,000, entitled “Development of Advanced Mass Spectrometric Approaches for the Analysis of the Proteome of Serum from Women with Pre-Eclampsia”.  This Fellowship enabled me to work with the Zubarev lab in Sweden and the Burlingame lab at UCSF on electron-mediated peptide dissociation methods.

My post-doctoral Research work from 2003-07 was in the Michael Barber Centre for Mass Spectrometry at Manchester University, supervised by Prof Simon Gaskell, on a project entitled “Investigation of the African trypanosome flagellar proteome”.

Prior to that, my PhD in Biochemistry & Molecular Biology was at the Ludwig Institute for Cancer Research, University College London. Supervised by Prof Michael Waterfield, the thesis title was “The Analysis of Protein Phosphorylation Sites using Affinity Chromatography and Mass Spectrometry”.

I direct the Keele University Mass Spectrometry Facility, based in the Huxley Building, alongside my research lab. Please feel free to contact me to discuss potential collaborations and service work.

ISTM Research theme: Healthcare Technologies

Link to: Keele Proteomics Mass Spectrometry Facility

My research interests are primarily in the field of biological mass spectrometry and its application to the improved understanding of biological and clinically-significant samples.

I have interests in the development of novel methods which improve our ability to process, analyse and derive valuable quantitative and qualitative information about the components of interest within complex biological samples. I have worked closely with industrial and academic partners to implement new methods in proteomics (Hart et al., J Am Soc Mass Spectrom., 2009).

 

I am interested in the gas-phase behaviour of large polypeptide ions (Lau et al., Rapid Commun. Mass Spectrom., 2009), and in differences between the behaviour of product ions generated using different methods for fragmenting these polypeptides (Hart et al., Methods Mol. Biol. 2010).

 ISTM_hart_fig3_790x600

 

ISTM_hart_fig3_790x600

 

 

Recent peer-reviewed publications:

1) Lau KW, Hart SR, Lynch JA, Wong SCC, Hubbard SJ, Gaskell SJ, “Observations on the Detection of b- and y-Type Ions in the Collisionally Activated Decomposition Spectra of Protonated Peptides”, Rapid Communications in Mass Spectrometry, 2009, 23, 10 1508-14. doi:10.1002/rcm.4032

2) Hart SR, Lau KW, Hao Z, Broadhead R, Portman N, Huhmer A, Gull K, McKean PG, Hubbard SJ, Gaskell SJ, “Analysis of the Trypanosome Flagellar Proteome using a Combined Electron Transfer Collisionally Activated Dissociation Strategy”, J. Am. Soc. Mass Spectrom., 2009, 20, 2 167-175. doi:10.1016/j.jasms.2008.08.014

3) Siepen JA, Swainston N, Jones AR, Hart SR, Hermjakob H, Jones P, Hubbard SJ, “An informatic pipeline for the data capture and submission of quantitative proteomic data using iTRAQTM”, Proteome Science, 2007, 5, 4. doi: 10.1186/1477-5956-5-4.

4) Castrillo JI, Zeef LA, Hoyle DC, Zhang N, Hayes A, Gardner DCJ, Cornell MJ, Petty J, Hakes L, Wardleworth L, Rash B, Brown M, Dunn WB, Broadhurst D, O'Donoghue K, Hester SS, Dunkley TP, Hart SR, Swainston, N, Li P, Gaskell SJ, Paton NW, Lilley KS, Kell DB, Oliver SG, “Growth control of the eukaryote cell: A systems biology study in yeast”, Journal of Biology, 2007, 6, 4. doi:10.1186/jbiol54

5) Myers JE, Hart SR, Armstrong S, Mires G, Beynon R, Gaskell SJ, Baker P, “There is no single circulating factor in pre-eclampsia (toxaemia)”, American Journal of Obstetrics and Gynaecology, 2007, 196, 266.e1-6. doi:10.1016/j.ajog.2006.10.875

6) Broadhead R, Dawe HR, Farr H, Griffiths S, Hart SR, Portman N, Shaw MK, Ginger ML, Gaskell SJ, McKean PG, Gull, K “Flagellar motility is required for the viability of the bloodstream trypanosome”, Nature, 2006, 440. 224-227. doi:10.1038/nature04541

7) Barnouin KN, Hart SR, Thompson AJ., Okuyama M, Waterfield MD, Cramer R, “Enhanced phosphopeptide isolation by Fe(III)-immobilised metal-ion affinity chromatography using 1,1,1,3,3,3-Hexafluoroisopropanol”, Proteomics 2005, 5: 4376-88. doi:10.1002/pmic.200401323

Book chapters

“A protocol for top down proteomics using HPLC and ETD/PTR-MS”, SR Hart, in Methods in Molecular Biology, P.R. Cutillas Ed., 2011.

“Distributions of ion series in ETD and CAD spectra: making a comparison”, SR Hart, KW Lau and SJ Hubbard, Methods in Molecular Biology 2010.

 

“Methods of proteome analysis: challenges and opportunities”, SR Hart and SJ Gaskell, in Practical Systems Biology, 2008. A Hetherington; C Grierson Eds., Taylor & Francis Ltd, UK. ISBN: 9780415407809.

Selected Publications

  • Hollamby MJ, Danks AE, Schnepp Z, Rogers SE, Hart SR, Nakanishi T. 2016. Fluorescent liquid pyrene derivative-in-water microemulsions. Chem Commun (Camb), vol. 52(46), 7344-7347. link> doi>
  • Hart SR, Baker PN, Kenny L, Myers JE. 2015. Electron transfer dissociation of native peptides facilitates enhanced identification of urinary peptides. International Journal of Mass Spectrometry, vol. 391, 41-46. doi>
  • Hart SR, Lau KW, Gaskell SJ, Hubbard SJ. 2011. Distributions of ion series in ETD and CID spectra: making a comparison. Methods Mol Biol, vol. 696, 327-337. link> doi>
  • HART S. 2010. A Protocol for Top-Down Proteomics Using HPLC and ETD/PTR-MS. In LC-MS/MS in Proteomics. Cutillas PR and Timms JF (Eds.). (vol. 658). doi>
  • Hart S, Blankley R, Guan S. 2009. Electron Transfer Dissociation Analysis of the Urinary Peptidome in Pregnancy. MOLECULAR & CELLULAR PROTEOMICS (p. S35). link>

Full Publications List show

Journal Articles

  • Hollamby MJ, Danks AE, Schnepp Z, Rogers SE, Hart SR, Nakanishi T. 2016. Fluorescent liquid pyrene derivative-in-water microemulsions. Chem Commun (Camb), vol. 52(46), 7344-7347. link> doi>
  • Hart SR, Baker PN, Kenny L, Myers JE. 2015. Electron transfer dissociation of native peptides facilitates enhanced identification of urinary peptides. International Journal of Mass Spectrometry, vol. 391, 41-46. doi>
  • Hart SR, Lau KW, Gaskell SJ, Hubbard SJ. 2011. Distributions of ion series in ETD and CID spectra: making a comparison. Methods Mol Biol, vol. 696, 327-337. link> doi>
  • Lau KW, Hart SR, Lynch JA, Wong SCC, Hubbard SJ, Gaskell SJ. 2009. Observations on the detection of b- and y-type ions in the collisionally activated decomposition spectra of protonated peptides. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 23(10), 1508-1514. link> doi>
  • Hart SR, Lau KW, Hao Z, Broadhead R, Portman N, Huehmer A, Gull K, McKean PG, Hubbard SJ, Gaskell SJ. 2009. Analysis of the Trypanosome Flagellar Proteome Using a Combined Electron Transfer/Collisionally Activated Dissociation Strategy. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, vol. 20(2), 167-175. link> doi>
  • Castrillo J, Zeef LA, Hoyle DC, Zhang N, Hayes A, Gardner DCJ, Cornell MJ, Petty J, Hakes L, Wardleworth L, Rash B, Brown M, Dunn WB, Broadhurst D, O'Donoghue K, Hester SS, Dunkely TP, Hart SR, Swainton N, Li P, Gaskell SJ, Paton NW, Lilley KS, Kell DB, Oliver SG. 2007. Growth control of the eukaryote cell: a systems biology study in yeast. Journal of Biology, vol. 6(4). doi>
  • Myers JE, Hart S, Armstrong S, Mires GJ, Beynon R, Gaskell SJ, Baker PN. 2007. Evidence for multiple circulating factors in preeclampsia. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, vol. 196(3), 266-268. link> doi>
  • Siepen JA, Swainston N, Jones AR, Hart SR, Hermjakob H, Jones P, Hubbard SJ. 2007. An informatic pipeline for the data capture and submission of quantitative proteomic data using iTRAQ (TM). PROTEOME SCIENCE, vol. 5, Article ARTN 4. link> doi>
  • Broadhead R, Dawe HR, Farr H, Griffiths S, Hart SR, Portman N, Shaw MK, Ginger ML, Gaskell SJ, McKean PG, Gull K. 2006. Flagellar motility is required for the viability of the bloodstream trypanosome. NATURE, vol. 440(7081), 224-227. link> doi>
  • Barnouin KN, Hart SR, Thompson AJ, Okuyama M, Waterfield MD, Cramer R. 2005. Enhanced phosphopeptide isolation by Fe(III)-IMAC using 1,1,1,3,3,3-hexafluoroisopropanol. PROTEOMICS, vol. 5(17), 4376-4388. doi>
  • Hart SR and Gaskell SJ. 2005. LC-tandem MS in proteome characterization. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, vol. 24(7), 566-575. link> doi>
  • Garwood K, McLaughlin T, Garwood C, Joens S, Morrison N, Taylor CF, Carroll K, Evans C, Whetton AD, Hart S, Stead D, Yin Z, Brown AJP, Hesketh A, Chater K, Hansson L, Mewissen M, Ghazal P, Howard J, Lilley KS, Gaskell SJ, Brass A, Hubbard SJ, Oliver SG, Paton NW. 2004. PEDRo: A database for storing, searching and disseminating experimental proteomics data. BMC GENOMICS, vol. 5, Article ARTN 68. link> doi>
  • Hart SR and Riba-Garcia I. 2004. The analysis of tandem mass spectrometric clatasets: high-throughput investigations require high-quality validation. DRUG DISCOVERY TODAY, vol. 9(9), 391-392. link> doi>
  • Thompson AJ, Hart SR, Franz C, Barnouin K, Ridley A, Cramer R. 2003. Characterization of Protein Phosphorylation by Mass Spectrometry Using Immobilized Metal Ion Affinity Chromatography with On-Resin β-Elimination and Michael Addition. Anal. Chem, vol. 75(13), 3232-3243. doi>
  • Hart SR, Waterfield MD, Burlingame AL, Cramer R. 2002. Factors governing the solubilization of phosphopeptides retained on ferric NTA IMAC beads and their analysis by MALDI TOFMS. Journal of the American Society for Mass Spectrometry, vol. 13(9), 1042-1051. doi>
  • Bruce LJ, Wrong O, Toye AM, Young MT, Ogle G, Ismail Z, Sinha AK, McMaster P, Hwaihwanje I, Nash GB, Hart S, Lavu E, Palmer R, Othman A, Unwin RJ, Tanner MJ. 2000. Band 3 mutations, renal tubular acidosis and South-East Asian ovalocytosis in Malaysia and Papua New Guinea: loss of up to 95% band 3 transport in red cells. Biochem, vol. 350, 41-51. doi>
  • Hart SR. Electron transfer dissociation of native peptides facilitates enhanced identification of urinary peptides. International Journal of Mass Spectrometry. doi>

Chapters

  • HART S. 2010. A Protocol for Top-Down Proteomics Using HPLC and ETD/PTR-MS. In LC-MS/MS in Proteomics. Cutillas PR and Timms JF (Eds.). (vol. 658). doi>
  • Hart SR and Gaskell SJ. 2008. Methods of proteome analysis: challenges and opportunities. In Practical systems biology. Grierson C and Hetherington AM (Eds.). Taylor & Francis Group.

Other

  • Hart S, Blankley R, Guan S. 2009. Electron Transfer Dissociation Analysis of the Urinary Peptidome in Pregnancy. MOLECULAR & CELLULAR PROTEOMICS (p. S35). link>
  • Myers JE, Hart S, Baker PN, Gaskell SJ. 2006. The development of iTRAQ as a tool for the identification of differentially expressed plasma proteins in preeclampsia and normal pregnancy. JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION (vol. 13, p. 125A). link>
  • Myers J, Hart S, Gaskell S, Baker P. 2005. Preliminary characterisation of the circulating factor(s) in preeclampsia using size exclusion and multiple affinity removal chromatography. JOURNAL OF THE SOCIETY FOR GYNECOLOGIC INVESTIGATION (vol. 12, p. 148A). link>
  • Hart S, Kenny L, Myers J, Baker P. 2015. [203-POS]: Electron transfer dissociation for native peptide fragmentation facilitates enhanced identification of urinary peptides and proteins in pregnancy. Pregnancy Hypertens (vol. 5, p. 102). link> doi>

At Keele I am Research Strand Lead within the Medical School, and am closely involved with the INSPIRE team to embed research awareness in undergraduate medical education. I co-lead Unit 1, Year 1 (Emergencies) and am co-lead for the Year 1 Publication Based Paper. I am a tutor for Problem-Based Learning, Professional Development (PDT) and Enhanced Professional and Academic Student Support (EPASS), providing academic and pastoral support to students in preclinical years.

As a Post-Doc and then Research Fellow at the Michael Barber Centre for Mass Spectrometry, Manchester, I managed and delivered the successful CPD course ‘Mass Spectrometry for Protein & Peptide Scientists’. I taught on the MRes in Post-Genome Science, and was module co-ordinator for programs on Manchester’s Doctoral Training Centre in Systems Biology and MRes in Translational Medicine.

Memberships of professional organisations and external roles:

I was awarded Membership of the Royal Society for Chemistry (MRSC) in 2006.

I am an elected member of the British Mass Spectrometry Society committee and am currently Education secretary, heading the Education sub-committee.

I am a member of the American Society for Mass Spectrometry and the Biochemical Society.

I am also a registered STEM Ambassador, and regularly take part in public events promoting science and school events to help engage the next generation of young scientists, including Keele Community Day, I'm a Scientist, Get Me Out of Here! and regional Science Fairs.