Professor Paul Horrocks

Title: Professor of Molecular Parasitology
Phone: +44 (0)1782 733670
Email: p.d.horrocks@keele.ac.uk
Location: Huxley Building : 166
Role: ISTM Research theme: Therapeutics
Senior Lecturer, Research Group Leader, Year 4 MBChB colead
ISTM Postgraduate Research Director
Contacting me: Arrange an appointment by e-mail
paul horrocks

I graduated with a BSc (Hons) in Biochemistry and Applied Molecular Biology from the University of Manchester Institute of Science and Technology (UMIST) before going on to complete a PhD at the University of Edinburgh investigating the DNA replication machinery of the human malarial parasite Plasmodium falciparum. Between 1996 and 1998, I worked at the University of Würzburg in Germany where I started to become interested in understanding stage-specific gene regulation in P. falciparum as well as a molecular investigation of parasite’s virulence factor PfEMP1. In 1998 I returned to the UK to the Weatherall Institute of Molecular Medicine, University of Oxford, where I worked with Prof. Newbold on the Malaria Genome Project and the antigenic switching and cytoadhesive properties of PfEMP1. Between 2003 and 2005 I was located at at the Liverpool School of Tropical Medicine before moving to the Institute for Science and Technology at Keele University on a Royal Society Relocation Fellowship. In 2006 I was appointed a Lecturer in the School of Medicine and became involved in the development and delivery of a new MBChB curriculum. I have since had the role of colead for year 1 (Challenges to Health) and am currently year 4 colead (Advanced Clinical Experience). In 2009 I received a BBSRC New Investigator Award and was promoted to Senior Lecturer. I have been an ordinary member of council for the British Society of Parasitology (2009-2012) and Keele University Senate (2010-13), and am on the editorial board for Case Reports in Infectious Disease, ISRN Tropical Medicine and Malaria Research and Treatment

geimsa stained falciparum

ISTM Research theme: Therapeutics

I lead one of the six research groups within the Institute for Science and Technology in Medicine that study the biology of the human malarial parasite P. falciparum, its pathogenesis and transmission by the mosquito vector. My work is focussed on the biology of the malarial parasite – and in particular the intraerythrocytic stages on infection in the human host, the principle developmental stages responsible for the pathology of disease.

My research addresses the following areas;

  • An investigation of the molecular mechanisms that direct the epigenetic and genetic control of gene expression during intraerythrocytic development
  • The application of bioluminescence as a tool to study the action of antimalarial drugs
  • An exploration of the action of novel classes of antimalarial drugs

Control of gene expression during intraerythroctyic development is controlled though a variety of molecular mechanisms operating at the epigenetic and genetic levels (Horrocks et al. 2009). Our research interests focus on how these operate to regulate the absolute and temporal programme of expression of house-keeping genes. We employ the use of cell culture, laboratory and bioinformatics techniques to help us better understand their interplay.

Reporter gene studies explore the effect of deletion of sequences that flank genes (Wu et al. 2011) and are combined with bioinformatics analyses to determine the effect of nuclear trans-acting factor against putative cis-acting factors.

 

We have also modelled the spatio-temporal organisation of the large transcriptional units in P. falciparum to better understand how large transcripts are arrayed over the relatively compact genome (Russell et al., 2013). This not only provides some interesting insights into the selective pressures on the parasite’s genome, but has also been extended to facilitate a comparative analysis of intergenic space organisation in other Apicomplexan parasites that are important for both human and veterinarian health. The nature of the sequences in these intergenic regions have also been explored – and show some interesting features of spatial organisation around key landmarks for gene expression.

More recently, we have been exploring the application of bioluminescence as a tool to monitor various aspects of antimalarial drug activity (Hasenkamp et al., 2013). Comparison against fluorescence assays reveal the two assays share similar profiles, however, bioluminescence may offer attractive opportunities in the development of rate-of-kill and lethal dose estimations.

There is an urgent demand to explore the potential of novel classes of drugs for their potential as new leads for antimalarial development. My laboratory collaborates with colleagues at Keele and Nottingham Trent University in an exploration of the structure activity relationship for synthetic monoindole alkaloids (Horrocks et al., 2012) and chemically modified glycosaminoglycans. The latter, are particularly attractive as their putative mode of action during the inhibition of erythrocyte invasion may be exploited to explore similar invasion blocking activity for a range of Apicomplexan parasites.

Our laboratory collaborates closely with other members of Keele University (Life Sciences and Chemistry), nationally with Nottingham Trent University and Charnwood Molecular Ltd and internationally with colleagues at the Universities of Massachussetts at Lowell and California at Riverside. The lab currently has one PhD student (Mr Imran Ullah), one MPhil student (Ms Haddi Mbye) and currently at any time between 1-4 undergraduate students.

lab work

Previous laboratory members;

Postdoc: Dr Sandra Hasenkamp (2010-2013)

PhD:     Eleanor Wong (Graduated 2011)

MSc:    Caroline Marshall (2008), Rebecca Knight (2008), Adam Worton (2009), Adrian Martin (2010), Robyn Kent (2011), Jolande Jefferson (2012), Amy Roberts (2013), Rhiannon Blow (2013)

Undergraduate (Medicine):    Mohammed Quadri, Neil Amison, Daniel Monnery, Srdjan Milic (Wellcome Trust Biomedical Vacation Scholarship), Laura Denman, Oliver Devine (Wellcome Trust Biomedical Vacation Scholarship), John Nathan Spence, Joshua Kearsley, Rose O’ Duffy and Nicolas Ellerby.

Undergraduate (Life Sciences): Emma-Louise Meredith, Anthony Harper, Adam Sidaway (Nuffield Foundation Summer Scholarship), Richard Roye, Taiwo Frank-Isewon, Cynthia Ariko, Dalbir Kaur, Janalou Malpango, Helena Briggs and Emma Knight.

Visitors: Mathilde Andre and Mélanie Rault (both IUT Saint Brieuc, France)

Horrocks laboratory photowall:

photowall

Our laboratory is supported financially by awards from the Biotechnology and Biological Sciences Research Council, Royal Society, North Staffordshire Medical Institute, Keele University, Wellcome Trust and Nuffield Foundation.

If you are interested in exploring opportunities to join my research laboratory, please contact me at p.d.horrocks@keele.ac.uk.

 

Selected Publications

  • Ullah I, Sharma R, Biagini GA, Horrocks P. 2017. A validated bioluminescence-based assay for the rapid determination of the initial rate of kill for discovery antimalarials. J Antimicrob Chemother, vol. 72(3), 717-726. link> doi>
  • Van Voorhis WC, Adams JH, Adelfio R, Ahyong V, Akabas MH, Alano P, Alday A, Alemán Resto Y, Alsibaee A, Alzualde A, Andrews KT, Avery SV, Avery VM, Ayong L, Baker M, Baker S, Ben Mamoun C, Bhatia S, Bickle Q, Bounaadja L, Bowling T, Bosch J, Boucher LE, Boyom FF, Brea J, Brennan M, Burton A, Caffrey CR, Camarda G, Carrasquilla M, Carter D, Belen Cassera M, Chih-Chien Cheng K, Chindaudomsate W, Chubb A, Colon BL, Colón-López DD, Corbett Y, Crowther GJ, Cowan N, D'Alessandro S, Le Dang N, Delves M, DeRisi JL, Du AY, Duffy S, Abd El-Salam El-Sayed S, Ferdig MT, Fernández Robledo JA, Fidock DA, Florent I, Fokou PVT, Galstian A, Gamo FJ, Gokool S, Gold B, Golub T, Goldgof GM, Guha R, Guiguemde WA, Gural N, Guy RK, Hansen MAE, Hanson KK, Hemphill A, Hooft van Huijsduijnen R, Horii T, Horrocks P, Hughes TB, Huston C, Igarashi I, Ingram-Sieber K, Itoe MA, Jadhav A, Naranuntarat Jensen A, Jensen LT, Jiang RHY, Kaiser A, Keiser J, Ketas T, Kicka S, Kim S, Kirk K, Kumar VP, Kyle DE, Lafuente MJ, Landfear S, Lee N, Lee S, Lehane AM, Li F, Little D, Liu L, Llinás M, Loza MI, Lubar A, Lucantoni L, Lucet I, Maes L, Mancama D, Mansour NR, March S, McGowan S, Medina Vera I, Meister S, Mercer L, Mestres J, Mfopa AN, Misra RN, Moon S, Moore JP, Morais Rodrigues da Costa F, Müller J, Muriana A, Nakazawa Hewitt S, Nare B, Nathan C, Narraidoo N, Nawaratna S, Ojo KK, Ortiz D, Panic G, Papadatos G, Parapini S, Patra K, Pham N, Prats S, Plouffe DM, Poulsen S-A, Pradhan A, Quevedo C, Quinn RJ, Rice CA, Abdo Rizk M, Ruecker A, St Onge R, Salgado Ferreira R, Samra J, Robinett NG, Schlecht U, Schmitt M, Silva Villela F, Silvestrini F, Sinden R, Smith DA, Soldati T, Spitzmüller A, Stamm SM, Sullivan DJ, Sullivan W, Suresh S, Suzuki BM, Suzuki Y, Swamidass SJ, Taramelli D, Tchokouaha LRY, Theron A, Thomas D, Tonissen KF, Townson S, Tripathi AK, Trofimov V, Udenze KO, Ullah I, Vallieres C, Vigil E, Vinetz JM, Voong Vinh P, Vu H, Watanabe N-A, Weatherby K, White PM, Wilks AF, Winzeler EA, Wojcik E, Wree M, Wu W, Yokoyama N, Zollo PHA, Abla N, Blasco B, Burrows J, Laleu B, Leroy D, Spangenberg T, Wells T, Willis PA. 2016. Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond. PLoS Pathog, vol. 12(7), e1005763. link> doi>
  • Parry-Smith W, Sutherland A, Horrocks PD, O'Mahony F. 2015. Bullying and undermining: a threat to both patient care and medical personnel wellbeing? A cross sectional survey of the Undergraduates, trainees and consultants in Obstetrics and Gynaecology in the West Midlands. MedEd Publish. doi>
  • Aldulaimi O, Horrocks P, Li WW. 2015. Antimicrobial activities and chemical compositions of Pogostemon cablin (Blanco) Benth. and Perilla frutescens (L.) Britt. in a traditional Chinese medicine formula. Nutrition and Medicine (vol. 204, pp. 497-498). ISSN: 2195-0083. doi> link>
  • Horrocks PD, Russell K, Emes R. 2015. Triaging informative cis -regulatory elements for the combinatorial control of temporal gene expression during Plasmodium falciparum intraerythrocytic development. Parasites and Vector. doi>

Full Publications List show

Books

  • HORROCKS PD, Kyes S, Bull PC, Deitsch K. Sherman I (Ed.). 2005. Antigenic variation in human malaria. (First ed.). Washington DC: ASM Press. link>

Journal Articles

  • Ullah I, Sharma R, Biagini GA, Horrocks P. 2017. A validated bioluminescence-based assay for the rapid determination of the initial rate of kill for discovery antimalarials. J Antimicrob Chemother, vol. 72(3), 717-726. link> doi>
  • Van Voorhis WC, Adams JH, Adelfio R, Ahyong V, Akabas MH, Alano P, Alday A, Alemán Resto Y, Alsibaee A, Alzualde A, Andrews KT, Avery SV, Avery VM, Ayong L, Baker M, Baker S, Ben Mamoun C, Bhatia S, Bickle Q, Bounaadja L, Bowling T, Bosch J, Boucher LE, Boyom FF, Brea J, Brennan M, Burton A, Caffrey CR, Camarda G, Carrasquilla M, Carter D, Belen Cassera M, Chih-Chien Cheng K, Chindaudomsate W, Chubb A, Colon BL, Colón-López DD, Corbett Y, Crowther GJ, Cowan N, D'Alessandro S, Le Dang N, Delves M, DeRisi JL, Du AY, Duffy S, Abd El-Salam El-Sayed S, Ferdig MT, Fernández Robledo JA, Fidock DA, Florent I, Fokou PVT, Galstian A, Gamo FJ, Gokool S, Gold B, Golub T, Goldgof GM, Guha R, Guiguemde WA, Gural N, Guy RK, Hansen MAE, Hanson KK, Hemphill A, Hooft van Huijsduijnen R, Horii T, Horrocks P, Hughes TB, Huston C, Igarashi I, Ingram-Sieber K, Itoe MA, Jadhav A, Naranuntarat Jensen A, Jensen LT, Jiang RHY, Kaiser A, Keiser J, Ketas T, Kicka S, Kim S, Kirk K, Kumar VP, Kyle DE, Lafuente MJ, Landfear S, Lee N, Lee S, Lehane AM, Li F, Little D, Liu L, Llinás M, Loza MI, Lubar A, Lucantoni L, Lucet I, Maes L, Mancama D, Mansour NR, March S, McGowan S, Medina Vera I, Meister S, Mercer L, Mestres J, Mfopa AN, Misra RN, Moon S, Moore JP, Morais Rodrigues da Costa F, Müller J, Muriana A, Nakazawa Hewitt S, Nare B, Nathan C, Narraidoo N, Nawaratna S, Ojo KK, Ortiz D, Panic G, Papadatos G, Parapini S, Patra K, Pham N, Prats S, Plouffe DM, Poulsen S-A, Pradhan A, Quevedo C, Quinn RJ, Rice CA, Abdo Rizk M, Ruecker A, St Onge R, Salgado Ferreira R, Samra J, Robinett NG, Schlecht U, Schmitt M, Silva Villela F, Silvestrini F, Sinden R, Smith DA, Soldati T, Spitzmüller A, Stamm SM, Sullivan DJ, Sullivan W, Suresh S, Suzuki BM, Suzuki Y, Swamidass SJ, Taramelli D, Tchokouaha LRY, Theron A, Thomas D, Tonissen KF, Townson S, Tripathi AK, Trofimov V, Udenze KO, Ullah I, Vallieres C, Vigil E, Vinetz JM, Voong Vinh P, Vu H, Watanabe N-A, Weatherby K, White PM, Wilks AF, Winzeler EA, Wojcik E, Wree M, Wu W, Yokoyama N, Zollo PHA, Abla N, Blasco B, Burrows J, Laleu B, Leroy D, Spangenberg T, Wells T, Willis PA. 2016. Open Source Drug Discovery with the Malaria Box Compound Collection for Neglected Diseases and Beyond. PLoS Pathog, vol. 12(7), e1005763. link> doi>
  • Parry-Smith W, Sutherland A, Horrocks PD, O'Mahony F. 2015. Bullying and undermining: a threat to both patient care and medical personnel wellbeing? A cross sectional survey of the Undergraduates, trainees and consultants in Obstetrics and Gynaecology in the West Midlands. MedEd Publish. doi>
  • Horrocks PD, Russell K, Emes R. 2015. Triaging informative cis -regulatory elements for the combinatorial control of temporal gene expression during Plasmodium falciparum intraerythrocytic development. Parasites and Vector. doi>
  • Coultas JA, Bodasing N, Horrocks P, Cadwgan A. 2015. Lemierre's Syndrome: Recognising a Typical Presentation of a Rare Condition. Case Rep Infect Dis, vol. 2015, 797415. link> doi>
  • Parry-Smith W, Sutherland A, Morse L, Horrocks P, O'Mahony F. 2014. Do medical students, trainee doctors, and consultants in the West Midlands experience bullying and undermining in obstetrics and gynaecology?. BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, vol. 121, 42. link>
  • Russell K, Cheng C-H, Bizzaro JW, Ponts N, Emes RD, Le Roch K, Marx KA, Horrocks P. 2014. Homopolymer tract organization in the human malarial parasite Plasmodium falciparum and related Apicomplexan parasites. BMC Genomics, vol. 15, 848. link> doi>
  • Horrocks P, Pickard MR, Parekh HH, Patel SP, Pathak RB. 2013. Synthesis and biological evaluation of 3-(4-chlorophenyl)-4-substituted pyrazole derivatives. Org Biomol Chem, vol. 11(29), 4891-4898. link> doi>
  • Russell K, Hasenkamp S, Emes R, Horrocks P. 2013. Analysis of the spatial and temporal arrangement of transcripts over intergenic regions in the human malarial parasite Plasmodium falciparum. BMC Genomics, vol. 14, 267. link> doi>
  • Hasenkamp S, Russell K, Ullah I, Horrocks P. 2013. Functional analysis of the 5' untranslated region of the phosphoglutamase 2 transcript in Plasmodium falciparum. Acta Trop, vol. 127(1), 69-74. link> doi>
  • Hasenkamp S, Merrick CJ, Horrocks P. 2013. A quantitative analysis of Plasmodium falciparum transfection using DNA-loaded erythrocytes. Mol Biochem Parasitol, vol. 187(2), 117-120. link> doi>
  • Russell K, Hasenkamp S, Emes R, Horrocks PD. 2013. Anaylsis of the spatial and temporal arrangements of transcripts over intergenic regions in the human malarial parasite (Under Revision). BMC Genomics.
  • Hasenkamp S, Sidaway A, Devine O, Roye R, Horrocks P. 2013. Evaluation of bioluminescence-based assays of anti-malarial drug activity. Malar J, vol. 12, 58. link> doi>
  • Hasenkamp S, Russell KT, Horrocks P. 2012. Comparison of the absolute and relative efficiencies of electroporation-based transfection protocols for Plasmodium falciparum. Malar J, vol. 11, 210. link> doi>
  • Hasenkamp S, Wong EH, Horrocks P. 2012. An improved single-step lysis protocol to measure luciferase bioluminescence in Plasmodium falciparum. Malar J, vol. 11, 42. link> doi>
  • Horrocks P, Fallon S, Denman L, Devine O, Duffy LJ, Harper A, Meredith E-L, Hasenkamp S, Sidaway A, Monnery D, Phillips TR, Allin SM. 2012. Synthesis and evaluation of a novel series of indoloisoquinolines as small molecule anti-malarial leads. Bioorg Med Chem Lett, vol. 22(4), 1770-1773. link> doi>
  • Staines HM, Croft SL, Horrocks PD. 2011. Developing novel strategies to prevent parasitic infections. Expert Rev Anti Infect Ther, vol. 9(7), 783-785. link> doi>
  • Wong EH, Hasenkamp S, Horrocks P. 2011. Analysis of the molecular mechanisms governing the stage-specific expression of a prototypical housekeeping gene during intraerythrocytic development of P. falciparum. J Mol Biol, vol. 408(2), 205-221. link> doi>
  • Horrocks P, Wong E, Russell K, Emes RD. 2009. Control of gene expression in Plasmodium falciparum - ten years on. Mol Biochem Parasitol, vol. 164(1), 9-25. link> doi>
  • Ponts N, Yang J, Chung D-WD, Prudhomme J, Girke T, Horrocks P, Le Roch KG. 2008. Deciphering the ubiquitin-mediated pathway in apicomplexan parasites: a potential strategy to interfere with parasite virulence. PLoS One, vol. 3(6), e2386. link> doi>
  • Kyes S, Christodoulou Z, Pinches R, Kriek N, Horrocks P, Newbold C. 2007. Plasmodium falciparum var gene expression is developmentally controlled at the level of RNA polymerase II-mediated transcription initiation. Mol Microbiol, vol. 63(4), 1237-1247. link> doi>
  • Choi S-W, Keyes MK, Horrocks P. 2006. LC/ESI-MS demonstrates the absence of 5-methyl-2'-deoxycytosine in Plasmodium falciparum genomic DNA. Mol Biochem Parasitol, vol. 150(2), 350-352. link> doi>
  • Frankland S, Adisa A, Horrocks P, Taraschi TF, Schneider T, Elliott SR, Rogerson SJ, Knuepfer E, Cowman AF, Newbold CI, Tilley L. 2006. Delivery of the malaria virulence protein PfEMP1 to the erythrocyte surface requires cholesterol-rich domains. Eukaryot Cell, vol. 5(5), 849-860. link> doi>
  • Carret CK, Horrocks P, Konfortov B, Winzeler E, Qureshi M, Newbold C, Ivens A. 2005. Microarray-based comparative genomic analyses of the human malaria parasite Plasmodium falciparum using Affymetrix arrays. Mol Biochem Parasitol, vol. 144(2), 177-186. link> doi>
  • Horrocks P and Muhia D. 2005. Pexel/VTS: a protein-export motif in erythrocytes infected with malaria parasites. Trends Parasitol, vol. 21(9), 396-399. link> doi>
  • Lakshmanan V, Bray PG, Verdier-Pinard D, Johnson DJ, Horrocks P, Muhle RA, Alakpa GE, Hughes RH, Ward SA, Krogstad DJ, Sidhu ABS, Fidock DA. 2005. A critical role for PfCRT K76T in Plasmodium falciparum verapamil-reversible chloroquine resistance. EMBO J, vol. 24(13), 2294-2305. link> doi>
  • Horrocks P, Pinches RA, Chakravorty SJ, Papakrivos J, Christodoulou Z, Kyes SA, Urban BC, Ferguson DJP, Newbold CI. 2005. PfEMP1 expression is reduced on the surface of knobless Plasmodium falciparum infected erythrocytes. J Cell Sci, vol. 118(Pt 11), 2507-2518. link> doi>
  • Makanga M, Bray PG, Horrocks P, Ward SA. 2005. Towards a proteomic definition of CoArtem action in Plasmodium falciparum malaria. Proteomics, vol. 5(7), 1849-1858. link> doi>
  • HORROCKS PD, Christodoulou Z, Kyes, S, Pinches R. 2004. Variable var transition rates underlie antigenic variation in malaria. Proceedings of the National Academy of Sciences, vol. 101(30), 11129-11134. doi>
  • Horrocks P, Kyes S, Pinches R, Christodoulou Z, Newbold C. 2004. Transcription of subtelomerically located var gene variant in Plasmodium falciparum appears to require the truncation of an adjacent var gene. Mol Biochem Parasitol, vol. 134(2), 193-199. link> doi>
  • Kriek N, Tilley L, Horrocks P, Pinches R, Elford BC, Ferguson DJP, Lingelbach K, Newbold CI. 2003. Characterization of the pathway for transport of the cytoadherence-mediating protein, PfEMP1, to the host cell surface in malaria parasite-infected erythrocytes. Mol Microbiol, vol. 50(4), 1215-1227. link> doi>
  • Waller KL, Muhle RA, Ursos LM, Horrocks P, Verdier-Pinard D, Sidhu ABS, Fujioka H, Roepe PD, Fidock DA. 2003. Chloroquine resistance modulated in vitro by expression levels of the Plasmodium falciparum chloroquine resistance transporter. J Biol Chem, vol. 278(35), 33593-33601. link> doi>
  • Kyes SA, Christodoulou Z, Raza A, Horrocks P, Pinches R, Rowe JA, Newbold CI. 2003. A well-conserved Plasmodium falciparum var gene shows an unusual stage-specific transcript pattern. Mol Microbiol, vol. 48(5), 1339-1348. link> doi>
  • Hall N, Pain A, Berriman M, Churcher C, Harris B, Harris D, Mungall K, Bowman S, Atkin R, Baker S, Barron A, Brooks K, Buckee CO, Burrows C, Cherevach I, Chillingworth C, Chillingworth T, Christodoulou Z, Clark L, Clark R, Corton C, Cronin A, Davies R, Davis P, Dear P, Dearden F, Doggett J, Feltwell T, Goble A, Goodhead I, Gwilliam R, Hamlin N, Hance Z, Harper D, Hauser H, Hornsby T, Holroyd S, Horrocks P, Humphray S, Jagels K, James KD, Johnson D, Kerhornou A, Knights A, Konfortov B, Kyes S, Larke N, Lawson D, Lennard N, Line A, Maddison M, McLean J, Mooney P, Moule S, Murphy L, Oliver K, Ormond D, Price C, Quail MA, Rabbinowitsch E, Rajandream M-A, Rutter S, Rutherford KM, Sanders M, Simmonds M, Seeger K, Sharp S, Smith R, Squares R, Squares S, Stevens K, Taylor K, Tivey A, Unwin L, Whitehead S, Woodward J, Sulston JE, Craig A, Newbold C, Barrell BG. 2002. Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Nature, vol. 419(6906), 527-531. link> doi>
  • HORROCKS PD, Kriek N, Newbold CI, Pinches R. 2002. Stage-specific promoter activity from stably maintained episomes in Plasmodium falciparum. International Journal for Parasitology , vol. 32(10), 1203-1206. doi>
  • HORROCKS PD, Kriek N, Kyes S, Pinches R. 2002. Effect of var gene disruption on switching in Plasmodium falciparum. Molecular Microbiology, vol. 45(4), 1131-1141. doi>
  • Carucci DJ, Horrocks P, Gardner MJ. 2002. Purification of chromosomes from Plasmodium falciparum. Methods Mol Med, vol. 72, 235-240. link> doi>
  • Kyes S, Horrocks P, Newbold C. 2001. Antigenic variation at the infected red cell surface in malaria. Annu Rev Microbiol, vol. 55, 673-707. link> doi>
  • Bowman S and Horrocks P. 2000. Assessing the impact of Plasmodium falciparum genome sequencing. Microbes Infect, vol. 2(12), 1479-1487. link> doi>
  • Horrocks P and Newbold CI. 2000. Intraerythrocytic polyubiquitin expression in Plasmodium falciparum is subjected to developmental and heat-shock control. Mol Biochem Parasitol, vol. 105(1), 115-125. link> doi>
  • Horrocks P, Bowman S, Kyes S, Waters AP, Craig A. 2000. Entering the post-genomic era of malaria research. Bull World Health Organ, vol. 78(12), 1424-1437. link>
  • Bowman S, Lawson D, Basham D, Brown D, Chillingworth T, Churcher CM, Craig A, Davies RM, Devlin K, Feltwell T, Gentles S, Gwilliam R, Hamlin N, Harris D, Holroyd S, Hornsby T, Horrocks P, Jagels K, Jassal B, Kyes S, McLean J, Moule S, Mungall K, Murphy L, Oliver K, Quail MA, Rajandream MA, Rutter S, Skelton J, Squares R, Squares S, Sulston JE, Whitehead S, Woodward JR, Newbold C, Barrell BG. 1999. The complete nucleotide sequence of chromosome 3 of Plasmodium falciparum. Nature, vol. 400(6744), 532-538. link> doi>
  • Wunsch S, Horrocks P, Gekle M, Lanzer M. 1999. Single-cell in vivo measurements of ion concentrations within the intracellular parasite Plasmodium falciparum. PARASITOLOGY TODAY, vol. 15(5), 198-200. link> doi>
  • Wünsch S, Horrocks P, Gekle M, Lanzer M. 1999. Single-cell measurements of ion concentrations within the intracellular parasite. Parasitol Today, vol. 15(5), 198-200. link>
  • Berry C, Humphreys MJ, Matharu P, Granger R, Horrocks P, Moon RP, Certa U, Ridley RG, Bur D, Kay J. 1999. A distinct member of the aspartic proteinase gene family from the human malaria parasite Plasmodium falciparum. FEBS Lett, vol. 447(2-3), 149-154. link> doi>
  • Horrocks P and Lanzer M. 1999. Mutational analysis identifies a five base pair cis-acting sequence essential for GBP130 promoter activity in Plasmodium falciparum. Mol Biochem Parasitol, vol. 99(1), 77-87. link> doi>
  • Horrocks P and Lanzer M. 1999. Differences in nucleosome organization over episomally located plasmids coincides with aberrant promoter activity in P. falciparum. Parasitol Int, vol. 48(1), 55-61. link> doi>
  • Tosh K, Cheesman S, Horrocks P, Kilbey B. 1999. Plasmodium falciparum: stage-related expression of topoisomerase I. Exp Parasitol, vol. 91(2), 126-132. link> doi>
  • Horrocks P, Dechering K, Lanzer M. 1998. Control of gene expression in Plasmodium falciparum. Mol Biochem Parasitol, vol. 95(2), 171-181. link> doi>
  • Ji DD, Sultan AA, Chakrabarti D, Horrocks P, Doerig C, Arnot DE. 1998. An RCC1-type guanidine exchange factor for the Ran G protein is found in the Plasmodium falciparum nucleus. Mol Biochem Parasitol, vol. 95(1), 165-170. link> doi>
  • Cheesman S, Horrocks P, Tosh K, Kilbey B. 1998. Intraerythrocytic expression of topoisomerase II from Plasmodium falciparum is developmentally regulated. Mol Biochem Parasitol, vol. 92(1), 39-46. link> doi>
  • Sanchez CP, Horrocks P, Lanzer M. 1998. Is the putative chloroquine resistance mediator CG2 the Na+/H+ exchanger of Plasmodium falciparum?. Cell, vol. 92(5), 601-602. link> doi>
  • HORROCKS PD and Lanzer M. 1998. Transfection of Plasmodium: a new chapter in the molecular analysis of malaria. Parasitology International, vol. 47, 101-106.
  • Wiesner J, Jomaa H, Wilhelm M, Tony HP, Kremsner PG, Horrocks P, Lanzer M. 1997. Host cell factor CD59 restricts complement lysis of Plasmodium falciparum-infected erythrocytes. Eur J Immunol, vol. 27(10), 2708-2713. link> doi>
  • Fischer K, Horrocks P, Preuss M, Wiesner J, Wünsch S, Camargo AA, Lanzer M. 1997. Expression of var genes located within polymorphic subtelomeric domains of Plasmodium falciparum chromosomes. Mol Cell Biol, vol. 17(7), 3679-3686. link> doi>
  • Wünsch S, Sanchez C, Gekle M, Kersting U, Fischer K, Horrocks P, Lanzer M. 1997. A method to measure the cytoplasmic pH of single, living Plasmodium falciparum parasites. Behring Inst Mitt, 44-50. link>
  • Horrocks P and Kilbey BJ. 1996. Physical and functional mapping of the transcriptional start sites of Plasmodium falciparum proliferating cell nuclear antigen. Mol Biochem Parasitol, vol. 82(2), 207-215. link> doi>
  • Doerig CM, Parzy D, Langsley G, Horrocks P, Carter R, Doerig CD. 1996. A MAP kinase homologue from the human malaria parasite, Plasmodium falciparum. Gene, vol. 177(1-2), 1-6. link> doi>
  • Horrocks P, Jackson M, Cheesman S, White JH, Kilbey BJ. 1996. Stage specific expression of proliferating cell nuclear antigen and DNA polymerase delta from Plasmodium falciparum. Mol Biochem Parasitol, vol. 79(2), 177-182. link> doi>
  • Doerig C, Doerig C, Horrocks P, Coyle J, Carlton J, Sultan A, Arnot D, Carter R. 1995. Pfcrk-1, a developmentally regulated cdc2-related protein kinase of Plasmodium falciparum. Mol Biochem Parasitol, vol. 70(1-2), 167-174. link> doi>
  • Cheesman S, McAleese S, Goman M, Johnson D, Horrocks P, Ridley RG, Kilbey BJ. 1994. The gene encoding topoisomerase II from Plasmodium falciparum. Nucleic Acids Res, vol. 22(13), 2547-2551. link> doi>
  • Russell K, Cheng C-H, Bizzaro JW, Ponts N, Emes R, Marx KA, Le Roch K, Horrocks P. 2015. 23 Genome-wide and positional homopolymeric tract enrichment at promoter-exon boundaries and exclusion of nucleosomes in Plasmodium spp. and related parasites. J Biomol Struct Dyn, vol. 33 Suppl 1, 13. link> doi>
  • Russell K, Emes R, Horrocks P. 2015. Triaging informative cis-regulatory elements for the combinatorial control of temporal gene expression during Plasmodium falciparum intraerythrocytic development. Parasit Vectors, vol. 8, 81. link> doi>

Other

  • Aldulaimi O, Horrocks P, Li WW. 2015. Antimicrobial activities and chemical compositions of Pogostemon cablin (Blanco) Benth. and Perilla frutescens (L.) Britt. in a traditional Chinese medicine formula. Nutrition and Medicine (vol. 204, pp. 497-498). ISSN: 2195-0083. doi> link>
  • Ponts N, Yang J, Prudhomme J, Horrocks P, Le Roch K. 2008. The ubiquitin proteasome system as a new therapeutic approach for the treatment of the human malaria parasite. INTERNATIONAL JOURNAL FOR PARASITOLOGY (vol. 38, p. S62). link>

My primary teaching and learning role is delivered through the coleadership of the year 4 cohort in the School of Medicine. Year 4 students are located in a variety of healthcare providers throughout Staffordshire and Shropshire. I have extensive experience as a PBL tutor in the pre-clinical years 1 and 2 of Medicine.

I provide undergraduate projects (experimental and non-experimental) as well as honours essay titles for the School of Life Sciences. Here I also contribute to the delivery of the MSc in Molecular Parasitology and Vector Control in conjunction with partners at Manchester and Salford Universities.

  • Sept 2013 MPhil funding (12 months, £18500) for Haddijatou Mbye
  • March 2013 Bridging the Gaps MRC Centenary Award (12 months, £7000) coapplicant with WenWu-Li, Keele University
  • Feb 2013 Eric Reid Fund for Methodology Application, Biochemical Society (2 months, £1200)
  • Sept 2012 Keele University ACORN funding (36 months, £19500) PhD support for Imran Ullah
  • March 2012 Medical Research Council industrial CASE studentship with Charnwood Molecular Ltd (36 months £109,288) to Mark Skidmore (P Horrocks, S. Allin, EA Yates and MJ McKenzie as coinvestigators). Novel glycotherapeutics for Alzheimer's disease
  • May 2011 Royal Society Research Grant (12 months £14,870) to P. Horrocks and S Hasenkamp. Development of a rapid, simple and sensitive luciferase-based growth assay for the high throughput screening of antimalarial drugs.
  • May 2011 Wellcome Trust Biomedical Vacation Scholarship (2 months, £1440) Evaluation of a stably integrated luciferase reporter cassette in Plasmodium falciparum for use in high throughput screening of antimalarial drugs.
  • May 2011 Nuffield Foundation Summer Scheme (2 months, £1440 with Sandra Hasenkamp) Optimization of parameters for a luciferase-based luminescent assay for high throughput screening of antimalarial drug action.
  • Jan 2011 Keele University Innovation Funding (12 months £10,000) to M. Skidmore, S. Allin, P Horrocks, K Haxter and P Procter. Novel Therapeutics for Severe Malaria - chemically modified, carbohydrate based, polymeric inhibitors of rosetting.
  • Jan 2010 North Staffordshire Research Institute Award (24 months £7,100) to P Horrocks and S Allin. An evaluation of the novel bis-indole alkaloid buchtienine as an antimalarial drug
  • Dec 2009 BBSRC New Investigator Award (36 months, £428,431). Identification, validation and therapeutic potential of cis-trans interactions that direct coordinated gene expression in Plasmodium falciparum.
  • Apr 2009 Wellcome Trust Biomedical Vacation Scholarship (2 months, WTD004448, £1440) An evaluation of magnetic nanoparticle-mediated delivery of exogenous DNA into the human malarial parasite Plasmodium falciparum
  • Sept 2008 BBSRC DTG Award to Support Mrs Karen Russell (5 years, part-time PhD)
  • Jan 2008 North Staffordshire Research Institute Award (12 months £10,000) to SJ Chakravorty and P Horrocks. An investigation of the role of Plasmodium falciparum infected erythrocyte knobs in mediating endothelial damage during cytoadhesion.
  • Sept 2007 Royal Society Conference Award (A4504-A365) £923 to attend and present at Woods Hole Molecular Parasitology Meeting.
  • Sept 2005 Royal Society UK Relocation Fellowship (12 months, 502014.K515/LT/sm, £54,105) Histone modifications and their role in P. falciparum transcriptional regulation.

I welcome applications from students who wish to undertake PhD or MPhil postgraduate studies in my team. Former, and current, postgraduate students include UK nationals as well as overseas students from South Asia, the Middle East and West Africa, undertaking both full-time and part-time studies in my laboratory. Interested students can contact me directly on p.d.horrocks@keele.ac.uk to discuss potential projects as well as the application process for these degrees.

My current research interests are as indicated on the "Research and Scholarship" tab.  Previous postgraduate students have submitted theses (and published their work) on topics as diverse as the molecular mechanisms that control gene expression in the human malarial parasite, a comparative investigation of intergenic regions in apicomplexan parasites to structure activity relationship studies of pyrazoline drugs against malarial and leishmanial parasites.

Currently, we have students developing novel antimalarial drug assays, investigations of cell biology following lethal doses of antimalarial drug treatment and the screening of antiparasitic and antimicrobial agents from libraries of natural products (a collaboration with a UK Industrial Biotechnology partner). 

My team works closely with those of other investigators interested in parasite biology as part of the Centre for Applied Entomology and Parasitology (CAEP) providing a stimulating collegiate environment for postgraduate research students, regular contact with your supervisory team and access to modern research facilities.