Dr Karina Wright

Title: Lecturer in Orthopaedics and Tissue Engineering
Phone: +44 1691 404022
Email: karina.wright@rjah.nhs.uk
Location: Robert Jones & Agnes Hunt Orthopaedic Hospital, Oswestry SY10 7AG, UK
Role: ISTM Research theme: Regenerative Medicine
Contacting me: By phone or email please
Dr Karina Wright 220x159

In 2001 I obtained a BSc (Hons) in Biological Sciences with professional experience from Salford University. I commenced my PhD studies at the Robert Jones & Agnes Hunt (RJAH) Orthopaedic Hospital in Oswestry in 2005, and in 2009, was awarded my PhD in Biomedical Engineering from Keele University. Since then I have been employed as a Lecturer/Research Fellow/Research Associate of the Institute for Science & Technology in Medicine, Keele University based at the RJAH Hospital, Oswestry.

ISTM Research theme: Regenerative Medicine

RESEARCH

Biology & pathology of musculoskeletal disorders and spinal cord injury

Cell-based therapies for the repair of musculoskeletal and spinal cord tissues

Bone marrow and umbilical cord derived stem cell biology (mesenchymal and hematopoietic)

PUBLICATIONS

  • Wright KT, El Masri W, Osman A, Chowdhury J, Johnson WEB. Bone marrow for the treatment of spinal cord injury: mechanisms and clinical application. (2011) Stem Cells, Volume 29, 169-178.
  • Wright KT, Seabright RJ, Logan A, Lilly AJ, Khanim F, Bunce CM, Johnson WEB. Extracellular Nm23H1 stimulates neurite outgrowth from dorsal root ganglia neurons in vitro independently of nerve growth factor supplementation or its nucleoside diphosphate kinase activity. (2010). Biochemical and Biophysical Research Communications, Volume 398, 79-85.
  • Walter MN, Wright KT, Fuller HR, MacNeil S, Johnson WEB. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: an in vitro study of fibroblast and keratinocyte scratch assays. (2010) Experimental Cell Research, Volume 316, Number 7; 1271-1281. 
  • Wright KT, Griffiths G, Johnson WEB. A comparison of industrial high content analysis versus research-based manual analysis to assay the effects of mesenchymal stem cell conditioned medium on neurite outgrowth in vitro. (2010) Journal of Biomolecular Screening, Volume 15, Issue 5; 576-582.
  • Bajada S, Marshall MJ, Wright KT, Richardson JB, Johnson WEB. Decreased osteogenesis, increased cell senescence and elevated dickkopf-1 secretion in human fracture non union stromal cells. (2009) Bone. Volume 45, Issue 4; 726-735.
  • Wright KT, Griffiths G, Johnson WEB. A comparison of industrial high content analyses versus research lab-based methods to assay the effects of mesenchymal stem cell conditioned medium on neurite outgrowth in vitro. Abstract (2009) Neurorehabilitation and Neural Repair, Volume 23, Number 9, Page 984.
  • Chamberlain G, Wright KT, Rot A, Ashton BA, Middleton J. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. (2008) PLoS ONE, Volume 3; 1-6.
  • Wright KT, El Masri W, Osman A, Roberts S, Trivedi J, Ashton BA, Johnson WEB. The cell culture expansion of bone marrow stromal cells from humans with spinal cord injury: implications for future cell transplantation therapy. (2008) Spinal Cord, Volume 46; 811-817.
  • Wright KT, El Masri W, Osman A, Roberts S, Ashton BA, Johnson WEB. Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A. (2007) Biochemical and Biophysical Research Communications, Volume 354; 559-566.
  • Wright KT, Khanim F, Bunce CM, Johnson WEB. The nucleoside diphosphatase kinase, nm23H1, is expressed by human bone marrow stromal cells and stimulates and directs neurite outgrowth in vitro. Abstract (2007) Neurorehabilitation and Neural Repair, Volume 21, Number 6, Page 62.
  • Johnson WEB, Sivan S, Wright KT, Eisenstein SM, Maroudas A, Roberts S. Human intevertebral disc cells promote nerve growth over substrata of human intevertebral disc aggrecan. (2006) Spine, Volume 31, Number 11; 1106-1114.
  • Wright KT, El Masri W, Osman A, Roberts S, Ashton BA, Johnson WEB. Marrow stromal cells from spinal cord injured patients promote nerve growth and reduce the inhibitory effects of chondroitin sulphated proteoglycans in vitro. Abstract (2006) Journal of Spinal Cord Medicine, Volume 29, Number 3, Page 270.

Selected Publications

  • Hulme CH, Wilson EL, Peffers MJ, Roberts S, Simpson DM, Richardson JB, Gallacher P, Wright KT. 2017. Autologous chondrocyte implantation-derived synovial fluids display distinct responder and non-responder proteomic profiles. Arthritis Res Ther, vol. 19(1), 150. link> doi>
  • Richardson JB, Wright KT, Wales J, Kuiper JH, McCarthy HS, Gallacher P, Harrison PE, Roberts S. 2017. Efficacy and safety of autologous cell therapies for knee cartilage defects (autologous stem cells, chondrocytes or the two): randomized controlled trial design. Regen Med, vol. 12(5), 493-501. link> doi>
  • Ziya S, Medcalf N, El A, Wright KT. 2017. 210 - Facilitating the operational readiness of the NHS for the in-house manufacture and delivery of autologous cell therapy. Cytotherapy, vol. 19(5), S147. doi> link>
  • Wright KT, McCarthy H, Forsyth N, Roberts S. 2017. 283 - Characteristics of human bone marrow mscs are sensitive to mononuclear cell separation technique and oxygen tension. Cytotherapy, vol. 19(5), S197. doi> link>
  • Wright KT, McCarthy H, Forsyth N, Roberts S. 2017. CHARACTERISTICS OF HUMAN BONE MARROW MSCS ARE SENSITIVE TO MONONUCLEAR CELL SEPARATION TECHNIQUE AND OXYGEN TENSION. CYTOTHERAPY (vol. 19, p. S119). link>

Full Publications List show

Journal Articles

  • Hulme CH, Wilson EL, Peffers MJ, Roberts S, Simpson DM, Richardson JB, Gallacher P, Wright KT. 2017. Autologous chondrocyte implantation-derived synovial fluids display distinct responder and non-responder proteomic profiles. Arthritis Res Ther, vol. 19(1), 150. link> doi>
  • Richardson JB, Wright KT, Wales J, Kuiper JH, McCarthy HS, Gallacher P, Harrison PE, Roberts S. 2017. Efficacy and safety of autologous cell therapies for knee cartilage defects (autologous stem cells, chondrocytes or the two): randomized controlled trial design. Regen Med, vol. 12(5), 493-501. link> doi>
  • Ziya S, Medcalf N, El A, Wright KT. 2017. 210 - Facilitating the operational readiness of the NHS for the in-house manufacture and delivery of autologous cell therapy. Cytotherapy, vol. 19(5), S147. doi> link>
  • Wright KT, McCarthy H, Forsyth N, Roberts S. 2017. 283 - Characteristics of human bone marrow mscs are sensitive to mononuclear cell separation technique and oxygen tension. Cytotherapy, vol. 19(5), S197. doi> link>
  • Wright KT, Kuiper JH, Richardson JB, Gallacher P, Roberts S. 2017. The Absence of Detectable ADAMTS-4 (Aggrecanase-1) Activity in Synovial Fluid Is a Predictive Indicator of Autologous Chondrocyte Implantation Success. Am J Sports Med, vol. 45(8), 1806-1814. link> doi>
  • Hulme CH, Brown SJ, Fuller HR, Riddell J, Osman A, Chowdhury J, Kumar N, Johnson WE, Wright KT. 2017. The developing landscape of diagnostic and prognostic biomarkers for spinal cord injury in cerebrospinal fluid and blood. Spinal Cord, vol. 55(2), 114-125. link> doi>
  • Garcia J, Mennan C, McCarthy HS, Roberts S, Richardson JB, Wright KT. Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat. Stem cells international, vol. 2016, Article 6969726. doi> link>
  • Garcia J, Wright K, Roberts S, Kuiper JH, Mangham C, Richardson J, Mennan C. 2016. Characterisation of synovial fluid and infrapatellar fat pad derived mesenchymal stromal cells: The influence of tissue source and inflammatory stimulus. Sci Rep, vol. 6, 24295. link> doi>
  • Turner SA, Wright KT, Jones PN, Balain B, Roberts S. 2016. Temporal Analyses of the Response of Intervertebral Disc Cells and Mesenchymal Stem Cells to Nutrient Deprivation. Stem Cells Int, vol. 2016, 5415901. link> doi>
  • Roberts S, Evans H, Wright K, van Niekerk L, Caterson B, Richardson JB, Kumar KHS, Kuiper JH. 2015. ADAMTS-4 activity in synovial fluid as a biomarker of inflammation and effusion. Osteoarthritis Cartilage, vol. 23(9), 1622-1626. link> doi>
  • Walter MNM, Kohli N, Khan N, Major T, Fuller H, Wright KT, Kuiper J-H, Johnson WEB. 2015. Human mesenchymal stem cells stimulate EaHy926 endothelial cell migration: combined proteomic and in vitro analysis of the influence of donor-donor variability. J Stem Cells Regen Med, vol. 11(1), 18-24. link>
  • Wright KT, Uchida K, Bara JJ, Roberts S, El Masri W, Johnson WEB. 2014. Spinal motor neurite outgrowth over glial scar inhibitors is enhanced by coculture with bone marrow stromal cells. Spine J, vol. 14(8), 1722-1733. link> doi>
  • Wright KT, Mennan C, Fox H, Richardson JB, Banerjee R, Roberts S. 2013. Characterization of the cells in repair tissue following autologous chondrocyte implantation in mankind: a novel report of two cases. Regen Med, vol. 8(6), 699-709. link> doi>
  • Mennan C, Wright K, Bhattacharjee A, Balain B, Richardson J, Roberts S. 2013. Isolation and characterisation of mesenchymal stem cells from different regions of the human umbilical cord. Biomed Res Int, vol. 2013, 916136. link> doi>
  • Nakajima H, Uchida K, Guerrero AR, Watanabe S, Sugita D, Takeura N, Yoshida A, Long G, Wright KT, Johnson WEB, Baba H. 2012. Transplantation of Mesenchymal Stem Cells Promotes an Alternative Pathway of Macrophage Activation and Functional Recovery after Spinal Cord Injury. JOURNAL OF NEUROTRAUMA, vol. 29(8), 1614-1625. link> doi>
  • Wright KT, El Masri W, Osman A, Chowdhury J, Johnson WEB. 2011. Concise review: Bone marrow for the treatment of spinal cord injury: mechanisms and clinical applications. Stem Cells, vol. 29(2), 169-178. link> doi>
  • Wright KT, El Masri W, Osman A, Chowdhury J, Johnson WE. 2010. Bone Marrow for the Treatment of Spinal Cord Injury: Mechanisms and Clinical Application. Stem Cells. doi>
  • Wright KT, Seabright R, Logan A, Lilly AJ, Khanim F, Bunce CM, Johnson WEB. 2010. Extracellular Nm23H1 stimulates neurite outgrowth from dorsal root ganglia neurons in vitro independently of nerve growth factor supplementation or its nucleoside diphosphate kinase activity. Biochem Biophys Res Commun, vol. 398(1), 79-85. link> doi>
  • Wright KT, Griffiths GJ, Johnson WEB. 2010. A comparison of high-content screening versus manual analysis to assay the effects of mesenchymal stem cell-conditioned medium on neurite outgrowth in vitro. J Biomol Screen, vol. 15(5), 576-582. link> doi>
  • Walter MNM, Wright KT, Fuller HR, MacNeil S, Johnson WEB. 2010. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: an in vitro study of fibroblast and keratinocyte scratch assays. Exp Cell Res, vol. 316(7), 1271-1281. link> doi>
  • Bajada S, Marshall MJ, Wright KT, Richardson JB, Johnson WEB. 2009. Decreased osteogenesis, increased cell senescence and elevated Dickkopf-1 secretion in human fracture non union stromal cells. Bone, vol. 45(4), 726-735. link> doi>
  • Wright KT, Masri WE, Osman A, Roberts S, Trivedi J, Ashton BA, Johnson WEB. 2008. The cell culture expansion of bone marrow stromal cells from humans with spinal cord injury: implications for future cell transplantation therapy. Spinal Cord, vol. 46(12), 811-817. link> doi>
  • Chamberlain G, Wright K, Rot A, Ashton B, Middleton J. 2008. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS One, vol. 3(8), e2934. link> doi>
  • JOHNSON WEB, Osman A, Roberts S, Wright KT. 2007. Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A. Biochemical and Biophysical Research Communications, vol. 354(2), 559-566. doi>
  • Johnson WE, Sivan S, Wright KT, Eisenstein SM, Maroudas A, Roberts S. 2006. Human intervertebral disc cells promote nerve growth over substrata of human intervertebral disc aggrecan. Spine, vol. 31, 1187-1193. doi>
  • Kohli N, Wright KT, Sammons RL, Jeys L, Snow M, Johnson WEB. 2015. An In Vitro Comparison of the Incorporation, Growth, and Chondrogenic Potential of Human Bone Marrow versus Adipose Tissue Mesenchymal Stem Cells in Clinically Relevant Cell Scaffolds Used for Cartilage Repair. Cartilage, vol. 6(4), 252-263. link> doi>
  • Bara JJ, Turner S, Roberts S, Griffiths G, Benson R, Trivedi JM, Wright KT. 2015. High content and high throughput screening to assess the angiogenic and neurogenic actions of mesenchymal stem cells in vitro. Exp Cell Res, vol. 333(1), 93-104. link> doi>

Other

  • Wright KT, McCarthy H, Forsyth N, Roberts S. 2017. CHARACTERISTICS OF HUMAN BONE MARROW MSCS ARE SENSITIVE TO MONONUCLEAR CELL SEPARATION TECHNIQUE AND OXYGEN TENSION. CYTOTHERAPY (vol. 19, p. S119). link>
  • Ziya S, Medcalf N, El A, Wright KT. 2017. FACILITATING THE OPERATIONAL READINESS OF THE NHS FOR THE IN-HOUSE MANUFACTURE AND DELIVERY OF AUTOLOGOUS CELL THERAPY. CYTOTHERAPY (vol. 19, p. S89). link>
  • Hulme CH, Wilson EL, Peffers MJ, Peffers MJ, Fuller H, Roberts S, Simpson DM, Richardson JB, Gallacher P, Wright KT. 2017. POOR CLINICAL RESPONSE TO AUTOLOGOUS CHONDROCYTE IMPLANTATION IS ASSOCIATED WITH A UNIQUE SYNOVIAL FLUID PROTEOME PROFILE. OSTEOARTHRITIS AND CARTILAGE (vol. 25, p. S92). link> doi>
  • McCarthy HS, Wright KT, Williams JM, Gallacher P, Richardson JB, Roberts S. 2016. Analysis of cartilage oligomeric matrix protein in synovial fluid, plasma and repair tissue of patients treated with autologous chondrocyte implantation. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 97, pp. A11-A12). Wiley. link> doi> link>
  • Wright KT, Wilson E, Hulme C, Kuiper JH, Gallacher P, Richardson JB, Roberts S. 2016. Cartilage harvest elevates sCD14 and MMP-3 in the synovial fluid of the autologous chondrocyte implantation (ACI) treated joint. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 97, p. A24). link>
  • Turner SA, Wright KT, Trivedi J, Roberts S. 2014. The response of intervertebral disc cells and mesenchymal stromal cells to altered levels of glucose and serum in vitro. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 95, p. A34). link>
  • Lam KP, Dempsy KP, Smith WA, Wright KT, Masri WE, Richardson JB, IEEE. 2013. A Computational Approach to Quantifying Axon Regeneration in the Presence of Mesenchymal Stem Cells (MSCs). 2013 6TH INTERNATIONAL IEEE/EMBS CONFERENCE ON NEURAL ENGINEERING (NER) (pp. 1541-1544). link>
  • Jones P, Uchida K, Wright KT, Roberts S. 2011. Influence of small proteoglycans on nerve growth in the intervertebral disc. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 92, p. A12). link>
  • Johnson WEB, Wright KT, Roberts S, Bishop P. 2007. The small leucine-rich proteoglycan, opticin, is inhibitory to neurite extension and endothelial migration and is present in the neonatal intervertebral disc and vertebral growth plate. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 88, p. A65). link>

OTHER INFORMATION

Collaborators:

• Prof Sally Roberts
   Director of Spinal Research
   RJAH Orthopaedic Hospital

• Prof James Richardson
   Professor of Orthopaedic and Traumatic Surgery
   RJAH Orthopaedic Hospital

• Dr Eustace Johnson
   Professor of Stem Cells and Regenerative Biology
   Senior Faculty Postgraduate Research Tutor Faculty Research Co-ordinator Biological Sciences
   Faculty of Medicine Dentistry and Life Sciences
   University of Chester

• Dr Gareth Griffiths
   Director of Science
   Imagenbiotech
   University of Manchester

• Mr Wagih S El Masry
   Consultant Surgeon in Spinal Injuries
   Former President of International Spinal Cord Society (ISCOS)
   RJAH Orthopaedic Hospital

• Mr Aheed Osman
   Consultant Surgeon in Spinal Injuries
   Midland Centre for Spinal Injuries
   RJAH Orthopaedic Hospital

• Mr J Roy Chowdhury
   Consultant in Spinal Injuries & Rehabilitation Medicine
   Midlands Centre for Spinal Injuries
   RJAH Orthopaedic Hospital

 

PhD Students

John Garcia (Lead Supervisor 2013-2016)

Shiraz Ziya (Lead Supervisor 2014-present)

Soukaina Bahsoun (Co-supervisor 2016-present)

Jessica Sykes (Co-supervisor 2016-present)

Tim Hopkins (Co-supervisor 2016-present)

Amy Byrne (Co-supervisor 2016-present)

 

Equipment

BD FACS canto II flow cytometer (Arthritis Research UK Equipment Grant – PI)

Cell IQ II Live Cell Imaging Platform (Institute of Orthopaedics Equipment Grant – Co-I)

 

Funding

Arthritis Research UK Project Grant (PI)

Arthritis Research UK Tissue Engineering Centre (Co-I)

Medical Research Council Experimental Medicine Grant (Co-I)

Institute of Orthopaedics and Midlands Centre for Spinal Injuries (PI)

 

Team Members

Dr Charlotte Hulme (Post-Doctoral Research Associate FT)

Dr Sharon Owen (Post-Doctoral Research Associate PT)