Dr Adam Sharples

Title: Senior Lecturer in Cell and Tissue Engineering
Phone:
Email: a.p.sharples@keele.ac.uk
Location: Institute for Science and Technology in Medicine
Guy Hilton Research Centre
Thornburrow Drive
Hartshill
Stoke-on-Trent ST4 7QB
United Kingdom
Role: ISTM Research Theme: Regenerative Medicine
Contacting me: By phone or e-mail
Adam_Sharples_LJM2017_250x200

Dr Adam P Sharples is a Senior Lecturer and the course director for the MSc in Cell and Tissue Engineering.  His research is focused in cell/molecular biology, physiology and bioengineering, where he investigates the underlying cellular, molecular and epigenetic mechanisms of skeletal muscle growth and wasting in health and disease using both cell modelling and whole body approaches. He is an ex-professional Rugby League Player and founder and Editor-in-Chief of a new open access journal Cellular and Molecular Exercise Physiology

 

 

 Please visit The Sharples Muscle Lab website for full

 research activities: https://sharplesmusclelab.com

Selected Publications

  • Seaborne RA, Strauss J, Cocks M, Shepherd S, O'Brien TD, van Someren KA, Bell PG, Murgatroyd C, Morton JP, Stewart CE, Sharples AP. 2018. Human Skeletal Muscle Possesses an Epigenetic Memory of Hypertrophy. Sci Rep, vol. 8(1), 1898. link> doi> full text>
  • Fisher A, Seaborne RA, Hughes TM, Gutteridge A, Stewart CE, Coulson JM, Sharples AP, (Joint Corresponding Author), Jarvis JC. 2017. Transcriptomic and Epigenetic Regulation of Disuse Atrophy and the Return to Activity in Skeletal Muscle. FASEB Journal, vol. Epub Ahead of Print. link> doi> link> full text>
  • Kasper AM, Turner DC, Martin NRW, Sharples AP. 2018. Mimicking exercise in three-dimensional bioengineered skeletal muscle to investigate cellular and molecular mechanisms of physiological adaptation. J Cell Physiol, vol. 233(3), 1985-1998. link> doi> full text>
  • Girven M, Dugdale HF, Owens DJ, Hughes DC, Stewart CE, Sharples AP. 2016. L-glutamine Improves Skeletal Muscle Cell Differentiation and Prevents Myotube Atrophy After Cytokine (TNF-alpha) Stress Via Reduced p38 MAPK Signal Transduction. JOURNAL OF CELLULAR PHYSIOLOGY, vol. 231(12), 2720-2732. link> doi> full text>
  • Sharples AP, Stewart CE, Seaborne RA. 2016. Does skeletal muscle have an 'epi'-memory? The role of epigenetics in nutritional programming, metabolic disease, aging and exercise. AGING CELL, vol. 15(4), 603-616. link> doi> full text>

Full Publications List show

Journal Articles

  • Seaborne RA, Strauss J, Cocks M, Shepherd S, O'Brien TD, van Someren KA, Bell PG, Murgatroyd C, Morton JP, Stewart CE, Mein CA, Sharples AP. Methylome of human skeletal muscle after acute & chronic resistance exercise training, detraining & retraining. Scientific Data, vol. Accepted. In Press.
  • Impey SG, Hammond KM, Naughton R, Langan-Evans C, Shepherd SO, Sharples AP, Cegielski J, Smith K, Jeromson S, Hamilton DL, Close GL, Morton JP. 2018. Whey Protein Augments Leucinemia and Post-Exercise p70S6K1 Activity Compared to a Hydrolysed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability. International Journal of Sport Nutrition and Exercise Metabolism, vol. 0, 1-26. doi> link> full text>
  • Seaborne RA, Strauss J, Cocks M, Shepherd S, O'Brien TD, van Someren KA, Bell PG, Murgatroyd C, Morton JP, Stewart CE, Sharples AP. 2018. Human Skeletal Muscle Possesses an Epigenetic Memory of Hypertrophy. Sci Rep, vol. 8(1), 1898. link> doi> full text>
  • Dugdale HF, Hughes DC, Allan R, Deane CS, Coxon CR, Morton JP, Stewart CE, Sharples AP. 2018. The role of resveratrol on skeletal muscle cell differentiation and myotube hypertrophy during glucose restriction. Mol Cell Biochem, vol. 444(1-2), 109-123. link> doi> full text>
  • Brown AD, Close GL, Sharples AP, Stewart CE. 2017. Murine myoblast migration: influence of replicative ageing and nutrition. BIOGERONTOLOGY, vol. 18(6), 947-964. link> doi> full text>
  • Fisher A, Seaborne RA, Hughes TM, Gutteridge A, Stewart CE, Coulson JM, Sharples AP, (Joint Corresponding Author), Jarvis JC. 2017. Transcriptomic and Epigenetic Regulation of Disuse Atrophy and the Return to Activity in Skeletal Muscle. FASEB Journal, vol. Epub Ahead of Print. link> doi> link> full text>
  • Allan R, Sharples AP, Close GL, Drust B, Shepherd SO, Dutton J, Morton JP, Gregson W. 2017. Postexercise cold water immersion modulates skeletal muscle PGC-1α mRNA expression in immersed and nonimmersed limbs: evidence of systemic regulation. J Appl Physiol (1985), vol. 123(2), 451-459. link> doi> full text>
  • Sharples AP. 2017. Cellular and Molecular Exercise Physiology: A Historical Perspective for the Discovery of Mechanisms Contributing to Skeletal Muscle Adaptation. Cellular and Molecular Exercise Physiology, vol. 5(1). doi> full text>
  • Kasper AM, Turner DC, Martin NRW, Sharples AP. 2018. Mimicking exercise in three-dimensional bioengineered skeletal muscle to investigate cellular and molecular mechanisms of physiological adaptation. J Cell Physiol, vol. 233(3), 1985-1998. link> doi> full text>
  • Saini A, Sharples AP, Al-Shanti N, Stewart CE. 2017. Omega-3 fatty acid EPA improves regenerative capacity of mouse skeletal muscle cells exposed to saturated fat and inflammation. BIOGERONTOLOGY, vol. 18(1), 109-129. link> doi> full text>
  • Girven M, Dugdale HF, Owens DJ, Hughes DC, Stewart CE, Sharples AP. 2016. L-glutamine Improves Skeletal Muscle Cell Differentiation and Prevents Myotube Atrophy After Cytokine (TNF-alpha) Stress Via Reduced p38 MAPK Signal Transduction. JOURNAL OF CELLULAR PHYSIOLOGY, vol. 231(12), 2720-2732. link> doi> full text>
  • Hammond KM, Impey SG, Currell K, Mitchell N, Shepherd SO, Jeromson S, Hawley JA, Close GL, Hamilton LD, Sharples AP, Morton JP. 2016. Postexercise High-Fat Feeding Suppresses p70S6K1 Activity in Human Skeletal Muscle. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, vol. 48(11), 2108-2117. link> doi> full text>
  • Sharples AP, Stewart CE, Seaborne RA. 2016. Does skeletal muscle have an 'epi'-memory? The role of epigenetics in nutritional programming, metabolic disease, aging and exercise. AGING CELL, vol. 15(4), 603-616. link> doi> full text>
  • Sharples AP, Polydorou I, Hughes DC, Owens DJ, Hughes TM, Stewart CE. 2016. Skeletal muscle cells possess a 'memory' of acute early life TNF-alpha exposure: role of epigenetic adaptation. BIOGERONTOLOGY, vol. 17(3), 603-617. link> doi> full text>
  • Hughes DC, Stewart CE, Sculthorpe N, Dugdale HF, Yousefian F, Lewis MP, Sharples AP. 2016. Testosterone enables growth and hypertrophy in fusion impaired myoblasts that display myotube atrophy: deciphering the role of androgen and IGF-I receptors. BIOGERONTOLOGY, vol. 17(3), 619-639. link> doi> full text>
  • Impey SG, Hammond KM, Shepherd SO, Sharples AP, Stewart C, Limb M, Smith K, Philp A, Jeromson S, Hamilton DL, Morton JP. 2016. Fuel for the work required: A practical approach to amalgamating train-low paradigms for endurance athletes. Physiological Reports, vol. 4(10). doi> full text>
  • Owens DJ, Sharples AP, Donovan T, Tang J, Fraser WD, Morton JP, Stewart CE, Close GL. 2016. Vitamin D and Skeletal Muscle Regeneration: A Systems Approach. Japanese Journal of Physical Fitness and Sports Medicine, vol. 65(1), 157. doi>
  • Owens DJ, Sharples AP, Polydorou I, Alwan N, Donovan T, Tang J, Fraser WD, Cooper RG, Morton JP, Stewart C, Close GL. 2015. A systems-based investigation into vitamin D and skeletal muscle repair, regeneration, and hypertrophy. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, vol. 309(12), E1019-E1031. link> doi>
  • Sharples AP, Hughes DC, Deane CS, Saini A, Selman C, Stewart CE. 2015. Longevity and skeletal muscle mass: the role of IGF signalling, the sirtuins, dietary restriction and protein intake. AGING CELL, vol. 14(4), 511-523. link> doi> full text>
  • Sharples AP and Stewart CE. 2014. Comments on CrossTalk opposing view: The dominant mechanism causing disuse muscle atrophy is proteolysis. The Journal of Physiology, vol. 592(24), 5345-5347. link> doi> link>
  • Player DJ, Martin NRW, Passey SL, Sharples AP, Mudera V, Lewis MP. 2014. Acute mechanical overload increases IGF-I and MMP-9 mRNA in 3D tissue-engineered skeletal muscle (vol 36, pg 1113, 2014). BIOTECHNOLOGY LETTERS, vol. 36(7), 1555-1556. link> doi>
  • Player DJ, Martin NRW, Passey SL, Sharples AP, Mudera V, Lewis MP. 2014. Acute mechanical overload increases IGF-I and MMP-9 mRNA in 3D tissue-engineered skeletal muscle. BIOTECHNOLOGY LETTERS, vol. 36(5), 1113-1124. link> doi>
  • Deane CS, Hughes DC, Sculthorpe N, Lewis MP, Stewart CE, Sharples AP. 2013. Impaired hypertrophy in myoblasts is improved with testosterone administration. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 138, 152-161. link> doi>
  • Deane CS, Hughes DC, Sculthorpe N, Lewis MP, Stewart CE, Sharples AP. 2013. Impaired hypertrophy in myoblasts is improved with testosterone administration. The Journal of Steroid Biochemistry and Molecular Biology, vol. 138(C), 152-161. doi>
  • Martin NRW, Passey SL, Player DJ, Khodabukus A, Ferguson RA, Sharples AP, Mudera V, Baar K, Lewis MP. 2013. Factors affecting the structure and maturation of human tissue engineered skeletal muscle. BIOMATERIALS, vol. 34(23), 5759-5765. link> doi>
  • Sharples AP, Al-Shanti N, Hughes DC, Lewis MP, Stewart CE. 2013. The role of insulin-like-growth factor binding protein 2 (IGFBP2) and phosphatase and tensin homologue (PTEN) in the regulation of myoblast differentiation and hypertrophy. GROWTH HORMONE & IGF RESEARCH, vol. 23(3), 53-61. link> doi>
  • Sharples AP, Player DJ, Martin NRW, Mudera V, Stewart CE, Lewis MP. 2012. Modelling in vivo skeletal muscle ageing in vitro using three-dimensional bioengineered constructs. AGING CELL, vol. 11(6), 986-995. link> doi>
  • Hughes DC, Sculthorpe N, Sharples AP, Lewis MP. 2012. Testosterone and molecular regulation of skeletal muscle. Perspectives on Anabolic Androgenic Steroids (AAS) and Doping in Sport and Health, 27-55.
  • Saini A, Al-Shanti N, Sharples A, Stewart CE. 2012. Sirtuin 1 regulates skeletal myoblast survival and enhances differentiation in the presence of resveratrol. EXPERIMENTAL PHYSIOLOGY, vol. 97(3), 400-418. link> doi>
  • Sharples AP, Al-Shanti N, Lewis MP, Stewart CE. 2011. Reduction of Myoblast Differentiation Following Multiple Population Doublings in Mouse C2C12 Cells: A Model to Investigate Ageing?. JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 112(12), 3773-3785. link> doi>
  • Sharples AP and Stewart CE. 2011. Myoblast models of skeletal muscle hypertrophy and atrophy. CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE, vol. 14(3), 230-236. link> doi>
  • Sharples AP, Al-Shanti N, Stewart CE. 2010. C-2 and C2C12 Murine Skeletal Myoblast Models of Atrophic and Hypertrophic Potential: Relevance to Disease and Ageing?. JOURNAL OF CELLULAR PHYSIOLOGY, vol. 225(1), 240-250. link> doi>
  • Tolfrey K, Doggett A, Boyd C, Pinner S, Sharples A, Barrett L. 2008. Postprandial triacylglycerol in adolescent boys: A case for moderate exercise. Medicine and Science in Sports and Exercise, vol. 40(6), 1049-1057. doi>
  • Tolfrey K, Doggett A, Boyd C, Pinner S, Sharples A, Barrett L. 2008. Postprandial triacylglycerol in adolescent boys: A case for moderate exercise. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, vol. 40(6), 1049-1057. link> doi>

Chapters

  • Turner DC, Kasper AM, Seaborne RA, Brown AD, Close GL, Murphy M, Stewart CE, Martin NRW, Sharples AP. Exercising Bioengineered Skeletal Muscle In Vitro: Biopsy to Bioreactor. In Myogenesis: Methods and Protocols. Beate Ronning S (Ed.). (vol. vol. 1889). Springer Nature.
  • Sharples AP, Seaborne RA, Stewart CE. 2017. Epigenetics of skeletal muscle aging. In Epigenetics of Aging and Longevity. Moskalev A and Vaiserman AM (Eds.). (vol. Translational Epigenetics Volume 4). Elsevier. link> doi> link>
  • Hughes D, Sculthorpe N, Sharples AP, Lewis MP. 2012. Testosterone and molecular regulation of skeletal muscle. In: Perspectives on Anabolic Androgenic Steroids (AAS) and Doping in Sport and Health, Nova Science Publishers ISBN 10: 1620812436. In Perspectives on Anabolic Androgenic Steroids (AAS) and Doping in Sport and Health. Grace F and Baker JS (Eds.). Nova Science Publishers. link> link>

Other

  • Seaborne RA. The Role of DNA Methylation in the Regulation of Skeletal Muscle Atrophy, Hypertrophy and Epigenetic ‘Memory’.
  • Turner D, Seaborne R, Stewart C, Murphy M, Jarvis J, Martin N, Sharples AP. Mechanical loading of murine bioengineered skeletal muscle mimics the transcriptional & epigenetic response of human skeletal muscle after exercise. European Cells and Materials (ECM) (vol. eCM Online Periodical, 2018, Collection 4, TCES Conference Abstracts (page 031), p. 31). European Cells & Materials Ltd. link> link>
  • Allan RJ. MECHANISTIC CONTROL OF THE COLDINDUCED AUGMENTATION OF THE TRANSCRIPTIONAL CO-ACTIVATOR PGC-1α.
  • Allan R and Mawhinney C. 2017. Is the ice bath finally melting? Cold water immersion is no greater than active recovery upon local and systemic inflammatory cellular stress in humans. doi>
  • Dugdale HF, Stewart CE, Sharples AP. 2016. Resveratrol enables myotube survival under nutrient stress. European Muscle Conference. Montpellier, France.
  • Dugdale HF. The Role of Resveratrol and Sirtuin1 in Skeletal Muscle Under a Nutrient Stress.
  • Hammond KM, Impey SG, Currell K, Mitchell N, Shepherd SO, Jeromson S, Hawley JA, Close GL, Hamilton LD, Sharples AP, Morton JP. 2016. Postexercise High-Fat Feeding Suppresses p70S6K1 Activity in Human Skeletal Muscle. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE (vol. 48, pp. 2108-2117). link> doi>
  • Allan R, Sharples AP, Close G, Drust B, Shepherd S, Fraser W, Mawhinnney C, Hammond K, Morton J, Gregson W. 2016. The acute cold-induced increase in PGC-1α is partially mediated systemically through increased β-adrenergic stimulation. Vienna, Austria.
  • 2015. Day 1. Free Communications – Physiology and Nutrition. Journal of Sports Sciences (vol. 33, pp. s5-s8). doi>
  • Owens DJ, Polydorou I, Alwan N, Fraser WD, Tang J, Morton J, Sharples AP, Stewart CE, Close GL. 2015. The Role of Vitamin D in Skeletal Muscle Repair: A Systems Biology Approach. Malmo, Sweden.
  • Dugdale HF, Stewart CE, Sharples AP. 2015. The role of SIRT1 in aged and glucose restricted skeletal muscle cells. Proceedings of the Physiological Society. Royal College of Physicians, Edinburgh, United Kingdom. link> link>
  • Sharples AP, Polydorou J, Hughes DC, Hughes TM, Stewart CE. 2015. Skeletal muscle myoblasts have a ‘memory’ of previous TNF-α insults: Role of DNA methylation. Proceedings of the Physiological Society, Accepted. Edinburgh, United Kingdom. link> link>
  • Wong FY, Morris S, Sharples AP, Low DA, Scott MA, Doran DA. 2015. Effect of 30 days oral purine and pyrimidine nucleotide supplementation upon repeated sprint ability performance. Liverpool, UK..
  • Hughes DC, Yousefian F, Sculthorpe N, Stewart CE, Lewis MP, Sharples AP. 2014. Testosterone restores an aged myoblast phenotype via increases in AR and Akt Phosphorylation independently of upstream IGF-IR inhibition. Liverpool, UK..
  • Dugdale HF, Stewart CE, Sharples AP. 2014. The role of SIRTUIN1 in aged and calorie restricted skeletal muscle cells. Liverpool, UK..
  • Sharples AP, Hughes DC, Hughes TM, Stewart CE. 2013. Skeletal muscle myoblasts have a memory of previous TNF-α insults: Role of DNA methylation. Monte Verità, Ascona, Switzerland. link>
  • Potter C, Hughes D, Sharples A, Davies B, Dixon N, Tuttle J, Mauger A, Castle P, Christmas B, McNaughton L. 2013. Combined Effect Of Hyperhydration And Pre-Cooling On Endurance Cycling Performance In Hot And Humid Conditions. Medicine and Science in Sports and Exercise (vol. 5S, pp. 61-71). American College of Sports Medicine. link> link>
  • Deane C, Hughes DC, Sculthorpe N, Sharples AP. 2012. Testosterone rescues differentiation and hypertrophy in artificially aged skeletal muscle myoblasts. Oslo, Norway. link> link>
  • Sharples AP, Player DJ, Martin NRW, Stewart CE, Lewis MP. 2012. Mechanical Stretching of Artificially Aged Myoblasts in Physiologically Relevant 3-Dimensional Bioengineered constructs. Bruge, Belgium.
  • Hughes DC, Sculthorpe N, Sharples AP, Lewis MP. 2012. The effects of testosterone on molecular markers of hypertrophy in C2C12 skeletal muscle cells. Proc Physiol Soc (vol. 26, p. PC61). link> link>
  • Sharples AP, Al-Shanti N, Stewart CE. 2012. Tumour Necrosis Factor- Alpha (TNF-α) increases susceptibility to inhibition of differentiation in artificially aged muscle stem cells. University of Warwick.
  • Player DJ, Martin NRW, Castle PC, Passey S, Sharples AP, Mudera V, Lewis MP. 2011. Mechanical stimulation of 3D bio-engineered skeletal muscle. European Cells and Materials (vol. 22, p. 38).
  • Sharpies AP, Player DJ, Martin NRW, Passey S, Stewart CE, Lewis MP. 2011. Replicative ageing of myoblast in 3-D tissue engineered collagen constructs. European Cells and Materials (vol. 22, p. 39).
  • Player DJ, Martin NRW, Castle PC, Sharples AP, Passey S, Mudera V, Lewis MP. 2011. A putative model of endurance exercise using bio-engineered skeletal muscle. Proc Physiol Soc (vol. 23, p. PC333). link> link>
  • Sharples AP, Player DJ, Martin NRM, Passey S, Stewart CE, Lewis MP. 2011. Artificially aged skeletal myoblasts display reduced regeneration in bio-engineered skeletal muscle. Proc Physiol Soc (vol. 23, p. PC327). link> link>
  • Sharples AP, Al-Shanti N, Stewart CE. 2010. Differential responses of C2 and C2C12 mouse skeletal myoblasts in the absence or presence of tumour necrosis factor- alpha (TNF-α). Endocrine Abstracts (vol. 21, p. P127). Manchester, UK. link> link>

Teaching Expertise

Course Director MSc Cell and Tissue Engineering.

Research Grants Awarded 

  • GlaxoSmithKline, ‘Epi-Memory’ and its role in regeneration and recovery of human skeletal muscle, Dr. Adam Sharples (PI) Prof. Claire Stewart (Co-I) Dr. James Morton (Co-I) Grant value (£): 35,000, Duration of research project: 2 years
  • The Endocrinology Society UK, Equipment Grant: Novel Bioreactor to mimic exercise in in-vitro bioengineered muscle systems, Dr. Adam Sharples (PI), Grant value (£): 5,000, Duration of research project: 1 year
  • LJMU Faculty Studentship (Science/Engineering), Development of a novel optical fibre-based sensor to measure force in human in-vitro skeletal muscle systems., Dr Adam Sharples (DoS), Dr Mark Murphy, Dr. Frederick Bezombes, Grant value (£): 60,000, Duration of research project: 3 years
  • LJMU funded studentship, Novel approaches to the synthesis of multicyclic peptides: Chemical tools to investigate the role of IGF-I in skeletal muscle regeneration and degeneration, PhD Studentship Sanne Verhook, Dr. Chris Coxon (DoS), Dr. Adam Sharples (Supervisor), Professor Claire Stewart (Supervisor), Grant value (£): £57,867, Duration of research project: 3 years,
  • LJMU, 'Epigenetic regulation of skeletal muscle mass and performance: Does muscle have an ‘Epi-memory.’ PhD studentship: Robert Seaborne, Dr. Adam Sharples (DoS), Professor Claire Stewart, Dr. James Morton, Dr. Chris Murgatroyd, Grant value (£): £57,867, Duration of research project: 3 years,
  • GlaxoSmithKline (GSK) and LJMU, Post-damage Repair and Regeneration of Skeletal Muscle: Underlying Mechanisms? PhD Studentship Alex Brown, Professor Claire Stewart, Dr. Adam Sharples (Supervisor), Dr. Graeme Close (Supervisor), Grant value (£): £59,000 (30K GSK and 29K LJMU), Duration of research project: 3 years
  • Rugby Football Union (RFU) and LJMU, 'Personalising elite training through the use of individualised in-vitro model systems to simulate exercise.' PhD studentship Andreas Kasper, Dr. Adam Sharples (DoS), Dr. G. Close, Prof. Claire Stewart, Grant value (£): £67,500 (37.5K RFU, 30K LJMU), Duration of research project: 3 years.
  • Malaysian Government, Nucleotide supplementation in humans and high intensity repeated sprint exercise performance, muscle strength, damage and oxygen transport. PhD Studentship Fui Yen Wong, Dr. Dominic Doran (Dos), Dr. Adam Sharples (Supervisor), Dr. David Low (Supervisor), Grant value (£): 80,000, Duration of research project: 3 years
  • English Institute of Sport, Nutritional modulations of exercise-induced alterations in skeletal muscle oxidative capacity. PhD Studentship Kelly Hammond, Dr. Morton (PI), Dr. Close (supervisor), Dr Sharples (supervisor), Grant value (£): 30,000 EIS plus approx 28,000 (LJMU matched funding), Duration of research project: 3 years
  • Malaysian Government, Influence of training load on training responses and adaptation. PhD Studentship- Mohd Firdaus Bin Maasar, Prof Drust (DoS), Dr. Andrew Hulton (supervisor) Dr. Sharples (supervisor), Grant value (£): 67,616.00, Duration of research project: 3 years
  • The Physiological Society (Physoc) & LJMU, Does calorie restriction induce muscle wasting with age? Epigenetics and the cellular and molecular role of the Sirtuins, Dr. Adam Sharples, Grant value (£): £23,732 (10K Physoc, 13.7K LJMU), Duration of research project: 3 years