Dr Adam Sharples

Title: Senior Lecturer in Cell and Tissue Engineering
Phone:
Email: a.p.sharples@keele.ac.uk
Location: Institute for Science and Technology in Medicine
Guy Hilton Research Centre
Thornburrow Drive
Hartshill
Stoke-on-Trent ST4 7QB
United Kingdom
Role: ISTM Research Theme: Regenerative Medicine
Contacting me: By phone or e-mail
Adam_Sharples_LJM2017_250x200

Dr Adam P Sharples is a Senior Lecturer and the course director for the MSc in Cell and Tissue Engineering.  His research is focused in cell/molecular biology, physiology and bioengineering, where he investigates the underlying cellular, molecular and epigenetic mechanisms of skeletal muscle growth and wasting in health and disease using both cell modelling and whole body approaches. He is an ex-professional Rugby League Player and founder and Editor-in-Chief of a new open access journal Cellular and Molecular Exercise Physiology

 

 

Early Research and PhD

His early research centred on physiological adaptations of human skeletal muscle in response to exercise interventions in paediatric populations where he investigated muscle size, strength and neural activation following chronic resistance exercise as well as changes in metabolism after aerobic exercise. From this, he gained a fascination for the impact of lifespan and environmental encounters on skeletal muscle health.

During his PhD he went on to devise two cellular models of muscle stem cell ageing: 

http://onlinelibrary.wiley.com/doi/10.1002/jcp.22252/abstract Sharples et al., 2010 J Cell Physiol and 

http://onlinelibrary.wiley.com/doi/10.1002/jcb.23308/abstract, Sharples et al., 2011;  which resulted in an award for best oral presentation at the Berlin-Brandenburg School for Regenerative therapies, Max Planct Institute, Berlin, Germany (2010).

Post-doctoral Research

His PhD work formed the foundation of his post-doctoral studies developing three-dimensional (3D) bioengineered ‘mini-muscles’ in a cell culture environment that mimicked whole muscle tissue ageing.  He also helped to develop the first human skeletal ‘mini-muscles’ in culture from muscle derived adult stem cells and was involved in applying a system to mimic exercise in these bioengineered muscles; This resulted in publications in high tier journals such as Aging Cell (ranked 2/49 in geriatrics and gerontology, 5 year impact 7.1) and Biomaterials (ranked 1/32 in material science-biomaterials, 5 year impact 8.91).  

He has recently gained external funding from the Society for Endocrinology and the Rugby Football Union to undertake mechanical loading of bioengineered human muscle in novel bioreactor systems.

Appointed to Lecturer at LJMU in 2012:

In 2012 Dr. Sharples joined the UK’s leading Exercise Science Research Institute for Research Quality ((as determined by REF2014; see here) where he pursued research into the cellular, molecular and epigenetic mechanisms of muscle mass regulation using both cellular modelling and whole body experimentation with humans, including:

1)   The role of inflammation in muscle stem cell dysfunction: http://onlinelibrary.wiley.com/doi/10.1002/jcp.25380/abstract Girven et al., 2016 J Cell Physiol; 

       http://link.springer.com/article/10.1007%2Fs10522-016-9667-3 Saini et al., 2016 Biogerontology

       http://link.springer.com/article/10.1007/s10522-015-9604-x Sharples et al., 2015 Biogerontology

       http://onlinelibrary.wiley.com/doi/10.1113/expphysiol.2011.061028/pdf Saini et al., 2012 Exp Physiol.

 

2)   The role of nutritional interventions such as:

      - Caloric restriction: http://onlinelibrary.wiley.com/doi/10.1111/acel.12342/epdf Sharples et al., 2015 Aging Cell, 

      https://link.springer.com/article/10.1007%2Fs11010-017-3236-1 Dugdale et al., 2017 Mol Cell Biochem,

      - CHO restriction, fat and protein manipulation: http://physreports.physiology.org/content/4/10/e12803.long Impey et al., 2016 Physiol Reps, http://www.ncbi.nlm.nih.gov/pubmed/27327024 Hammond et al., 2016 MSSE https://www.ncbi.nlm.nih.gov/pubmed/29757056 Impey et al., 2018 IJSNEM

      -  Glutamine administration: http://onlinelibrary.wiley.com/doi/10.1002/jcp.25380/abstract Girven et al., 2016 J Cell Physiol

      - Vitamin D: http://ajpendo.physiology.org/content/309/12/E1019.long Owens et al., 2015 Am J Physiol Endo Metab

      - Omega 3 fatty acid: http://link.springer.com/article/10.1007%2Fs10522-016-9667-3 Saini et al., 2016 Biogerontology

      - HMB and Leucine: https://www.ncbi.nlm.nih.gov/pubmed/29110174 Brown et al., 2017 Biogerontology

       and resveratrol: http://onlinelibrary.wiley.com/doi/10.1113/expphysiol.2011.061028/pdf Saini et al., 2012 Exp         Physiol, https://link.springer.com/article/10.1007%2Fs11010-017-3236-1 Dugdale et al., 2017 Mol Cell Biochem.

 

3)   The role of testosterone and androgen receptor in ageing muscle cells 

      http://www.sciencedirect.com/science/article/pii/S0960076013000769 Deane/Hughes et al., 2013 J Steriod Biochem Mol Bol

      http://link.springer.com/article/10.1007/s10522-015-9621-9 Hughes et al., 2015 Biogerontology

4)   Gene knock-down (e.g. Phosphatase and tensin homologue/PTEN http://www.sciencedirect.com/science/article/pii/S109663741300021X?np=y 

Sharples et al., 2013 Growth Horm IGF Res.)

5)   Pharmacological activation/inhibition of SIRT1 (http://onlinelibrary.wiley.com/doi/10.1113/expphysiol.2011.061028/pdf Saini et al., 2012 Exp Physiol) 

      in order to activate anabolic and inactivate catabolic molecular pathways in skeletal muscle cells.

 

6)   He has also characterised a novel role for Insulin-like-Growth-Factor binding protein 2 (IGFBP2) in muscle cell differentiation and myofibre hypertrophy

(http://www.sciencedirect.com/science/article/pii/S109663741300021X?np=y Sharples et al., 2013 Growth Horm IGF Res.)

 

Appointed to Senior Lecturer at Keele University Research Institute for Science & Technology in Medicine in 2017

 

Cutting Edge Work

Most interestingly,  Dr. Sharples has recently derived some cellular data to suggest that muscle ‘remembers’ early life encounters of inflammatory cytokines via tagging of methylated regions of genes important with muscle regeneration in later life (http://link.springer.com/article/10.1007/s10522-015-9604-x Sharples et al., 2015 Biogerontology).  This led to collaboration with Professor Jonathan Jarvis investigating the epigenetics of muscle disuse atrophy (http://fasebj.org/doi/abs/10.1096/fj.201700089RR Fisher et al., 2017 FASEB J.) Most importantly this enabled recent funding from GlaxoSmithKline (GSK) and publications in Scientific Reports (Nature) (https://www.nature.com/articles/s41598-018-20287-3 Seaborne et al., 2018) identifying for the first time, that human skeletal muscle possesses an epigenetic memory, with the discovery of novel epigenetically regulated genes in skeletal muscle growth. This paper received international attention where the paper released by our press office received 39,000 reads on the university webpage, was picked up by over 20 media/news outlets including; Science Daily, Yahoo News and Newsweek, the story was also ran by IFLScience with 25 million followers on Facebook, and was the top story in Reddit on the day of the article with over 24,000 upvotes. The paper has now received 817 tweets on social media from 640 different users, with an upper bound of 1,319,554 followers and was featured on ABC radio in Australia.

 

Building on some of the themes to emerge from this work, his major research interests at Keele have three major strands:

i)    To understand the epigenetic mechanisms of muscle ‘memory’ in both human and cellular models and the role of this ‘memory’ in muscle repair after injury, from periods of muscle disuse, and muscle wasting in disease (such as cachexia & arthritis http://link.springer.com/article/10.1007/s10522-015-9604-x Sharples et al., 2015 Biogerontology; http://fasebj.org/doi/abs/10.1096/fj.201700089RR Fisher et al., 2017 FASEB J; https://www.nature.com/articles/s41598-018-20287-3 Seaborne et al., 2018). See reviews where we first define skeletal muscle Epigenetic Memory or ‘Epi-Memory here (Sharples et al., 2016 Aging Cell) and determine the key epigenetics in skeletal muscle aging (https://www.sciencedirect.com/science/article/pii/B978012811060700019X Sharples et al., 2018).  

ii)   Use in-vitro cellular models, including 3D bioengineering, to mimic in-vivo physiological environments in order to investigate mechanisms of muscle adaptation (e.g. reviewed in https://www.researchgate.net/publication/50989601_Myoblast_models_of_skeletal_muscle_hypertrophy_and_atrophy Sharples and Stewart, 2011 Curr Opin Nutr Metab Care; http://onlinelibrary.wiley.com/doi/10.1002/jcp.25840/abstract Kasper et al., 2017 J Cell Physiol).

iii)  To understand the molecular ‘cross-talk’ between energy status (particularly SIRT1) versus muscle growth signalling using human and cellular models (discussed in http://onlinelibrary.wiley.com/doi/10.1111/acel.12342/epdf Sharples et al., 2015 Aging Cell).

 

Overarching Aims and Objectives of Future Research:

Dr. Sharples'  future overarching research aims are to combine his expertise in human interventions and extensive working understanding of cellular and molecular muscle physiology to undertake ‘molecule to man’ based research in order to discover the molecular modulators of skeletal muscle growth and wasting with age and disease.  His overall objective is to provide therapeutic strategies and interventions to maximise muscle growth and minimise muscle loss to enable healthy muscle ageing across the lifespan.

 

Research Supervision

Current Postgraduate Research Supervisions:

2016:  PhD - Cellular and Molecular Mechanisms of Muscle Growth and Loss: Optical fibre sensor technology to measure force of human skeletal muscle using novel bioreactor systems that mimic exercise (Director of Studies, Daniel Turner, Full Time). Outputs: http://onlinelibrary.wiley.com/doi/10.1002/jcp.25840/full PMID: 28158895.

2015:  PhD - Novel approaches to the synthesis of multicyclic peptides: Chemical tools to investigate the role of IGF-I in skeletal muscle regeneration and degeneration (Supervisor, Sanne Verhook, Full Time).

2015:  PhD – Epigenetic regulation of skeletal muscle mass and performance: Does muscle have an ‘Epi-memory’? (Director of Studies, Robert Seaborne). Outputs: 

http://onlinelibrary.wiley.com/doi/10.1111/acel.12486/epdf PMID: 27102569; http://fasebj.org/doi/abs/10.1096/fj.201700089RR Fisher et al., 2017 FASEB J;

https://www.nature.com/articles/s41598-018-20287-3 Seaborne et al., 2018; https://www.sciencedirect.com/science/article/pii/B978012811060700019X Sharples et al., 2018.

2015:  PhD –  Personalising elite training through the use of individualised in-vitro model systems to simulate exercise (Director of Studies, Andreas Kasper). Outputs: http://onlinelibrary.wiley.com/doi/10.1002/jcp.25840/full PMID: 28158895.

2015:  PhD – Repair and regeneration of skeletal muscle after damage - the role and mechanisms of anti-inflammatory drugs in humans (Supervisor, Alex Brown, Full Time). Outputs: https://www.ncbi.nlm.nih.gov/pubmed/29110174 Brown et al., 2017.

2015:  PhD - Manipulating carbohydrate availability and calorie restriction to enhance exercise induced mitochondrial biogenesis within human skeletal muscle (Supervisor, Mark Heariss, Full Time).

2015:  PhD – Influence of training load on training responses and adaptation. (Supervisor, Mohd Firdaus Bin Maasar, Full-Time).

2014:  PhD – The effects of reduced carbohydrate and high protein availability on training-induced mitochondrial biogenesis (Supervisor, Kelly Hammond, Full-Time).                      Outputs: https://www.ncbi.nlm.nih.gov/pubmed/27327024 (PMID: 27327024)

2013:  PhD – Nucleotide supplementation in humans and high intensity repeated sprint exercise performance, muscle strength, damage and oxygen transport (Supervisor, Fiu Yen Wong, Full-Time). Outputs: http://www.tandfonline.com/doi/full/10.1080/02640414.2015.1110312 .

2013:  PhD – Nutritional modulation of training-induced alterations in mitochondrial biogenesis (Advisor, Sam Impey, Full-Time, Thesis Submitted).

 

Completed Postgraduate Research

2018:  PhD - Nutritional modulation of training-induced alterations in mitochondrial biogenesis (Advisor, Sam Impey, Full-Time, Thesis Submitted). Outputs: 

http://physreports.physiology.org/content/4/10/e12803 PMID: 27225627; https://www.ncbi.nlm.nih.gov/pubmed/27327024 PMID: 27327024; https://www.ncbi.nlm.nih.gov/pubmed/29757056 Impey et al., 2018 IJSNEM.

2017:  PhD – The effects of post-exercise cold-water immersion on training-induced mitochondrial biogenesis (Supervisor, Rob Allen, Full-Time). Outputs: http://onlinelibrary.wiley.com/doi/10.1002/jcp.25380/abstract

           http://onlinelibrary.wiley.com/doi/10.1113/JP273796/abstract PMID:27991663' 

           http://jap.physiology.org/content/early/2017/05/24/japplphysiol.00096.2017 PMID: 28546467

2017:  PhD thesis entitled: The role of resveratrol and SIRT1 in muscle under nutrient stress (Director of Studies, Hannah Dugdale). Outputs: http://onlinelibrary.wiley.com/doi/10.1002/jcp.25380/abstract PMID: 26991744; http://link.springer.com/article/10.1007/s10522-015-9621-9 Dugdale et al., 2017 Mol Cell Biochem.

2015:  PhD thesis entitled: 'Vitamin D and skeletal muscle: insights into function and regeneration.' (Academic Advisor, Daniel Owens). Outputs: http://ajpendo.physiology.org/content/early/2015/10/20/ajpendo.00375.2015 PMID; 26506852; http://onlinelibrary.wiley.com/doi/10.1002/jcp.25380/abstract PMID: 26991744; http://link.springer.com/article/10.1007%2Fs10522-015-9604-x    PMID: 26349924.

2014:  PhD thesis entitled: ‘The molecular regulation of testosterone and androgen receptor in skeletal muscle cells.’ (Director of Studies, David Hughes). Outputs:

http://www.sciencedirect.com/science/article/pii/S0960076013000769 PMID: 23714396; http://link.springer.com/article/10.1007/s10522-015-9621-9              PMID: 26538344; http://link.springer.com/article/10.1007%2Fs10522-015-9604-x PMID:  26349924.

2013:  PhD thesis entitled: ‘A tissue engineered human skeletal muscle model for use in exercise sciences.’ (Advisor, Neil Martin) Outputs: http://www.sciencedirect.com/science/article/pii/S0142961213004468 PMID: 23643182;  http://onlinelibrary.wiley.com/doi/10.1111/j.1474-9726.2012.00869.x/epdPMID: 22882433 & http://link.springer.com/article/10.1007/s10529-014-1464-y PMID: 24563297.

2013:  PhD thesis entitled: ‘An in-vitro model for assessment of skeletal muscle adaptation following exercise related cues.’ (Advisor, Darren Player). Outputs: http://link.springer.com/article/10.1007/s10529-014-1464-y PMID: 24563297; http://onlinelibrary.wiley.com/doi/10.1111/j.1474-9726.2012.00869.x/epdf 

PMID: 22882433 & http://www.sciencedirect.com/science/article/pii/S0142961213004468 PMID: 23643182.

Dr. Sharples has reviewed original research articles in journals such as: 

  • Nature Communications, PLoS ONE, AGE, Frontiers Physiology, Journal of Clinical Endocrinology and Metabolism, American Journal of Physiology - Cell Physiology, Endocrine, Journal of Cellular Physiology, BMC Genomics, Biogerontology, Journal of Applied Physiology, Molecular and Cellular Endocrinology, Journal of Cellular Biochemistry, Physiological Reports, BMC Cell Biology, Tissue Engineering, Journal of Anatomy, Cell Proliferation, Journal of Sport Sciences, Growth Hormone and IGF Research, Clinical Interventions in Aging, Journal of Cell Biochemistry and Function, Molecular Nutrition and Food Research, Biochemistry and Biophysics Reports.

        Adam has reviewed book chapters for:

Routledge (Taylor and Francis) and Elsevier Publishers 

 

Dr Sharples has reviewed external grant applications for:

European Research Council (ERC), Medical Research Council (MRC), Biotechnology and Biological Research Council (BBSRC) and Kidney Research UK.

 

Teaching Expertise:


Course Director MSc Cell and Tissue Engineering 

Selected Publications

  • Seaborne RA, Strauss J, Cocks M, Shepherd S, O'Brien TD, van Someren KA, Bell PG, Murgatroyd C, Morton JP, Stewart CE, Sharples AP. 2018. Human Skeletal Muscle Possesses an Epigenetic Memory of Hypertrophy. Sci Rep, vol. 8(1), 1898. link> doi> full text>
  • Fisher A, Seaborne RA, Hughes TM, Gutteridge A, Stewart CE, Coulson JM, Sharples AP, (Joint Corresponding Author), Jarvis JC. 2017. Transcriptomic and Epigenetic Regulation of Disuse Atrophy and the Return to Activity in Skeletal Muscle. FASEB Journal, vol. Epub Ahead of Print. link> doi> link> full text>
  • Kasper AM, Turner DC, Martin NRW, Sharples AP. 2018. Mimicking exercise in three-dimensional bioengineered skeletal muscle to investigate cellular and molecular mechanisms of physiological adaptation. J Cell Physiol, vol. 233(3), 1985-1998. link> doi> full text>
  • Girven M, Dugdale HF, Owens DJ, Hughes DC, Stewart CE, Sharples AP. 2016. L-glutamine Improves Skeletal Muscle Cell Differentiation and Prevents Myotube Atrophy After Cytokine (TNF-alpha) Stress Via Reduced p38 MAPK Signal Transduction. JOURNAL OF CELLULAR PHYSIOLOGY, vol. 231(12), 2720-2732. link> doi> full text>
  • Sharples AP, Stewart CE, Seaborne RA. 2016. Does skeletal muscle have an 'epi'-memory? The role of epigenetics in nutritional programming, metabolic disease, aging and exercise. AGING CELL, vol. 15(4), 603-616. link> doi> full text>

Full Publications List show

Journal Articles

  • Impey SG, Hammond KM, Naughton R, Langan-Evans C, Shepherd SO, Sharples AP, Cegielski J, Smith K, Jeromson S, Hamilton DL, Close GL, Morton JP. 2018. Whey Protein Augments Leucinemia and Post-Exercise p70S6K1 Activity Compared to a Hydrolysed Collagen Blend When in Recovery From Training With Low Carbohydrate Availability. International Journal of Sport Nutrition and Exercise Metabolism, vol. 0, 1-26. doi> link> full text>
  • Seaborne RA, Strauss J, Cocks M, Shepherd S, O'Brien TD, van Someren KA, Bell PG, Murgatroyd C, Morton JP, Stewart CE, Sharples AP. 2018. Human Skeletal Muscle Possesses an Epigenetic Memory of Hypertrophy. Sci Rep, vol. 8(1), 1898. link> doi> full text>
  • Dugdale HF, Hughes DC, Allan R, Deane CS, Coxon CR, Morton JP, Stewart CE, Sharples AP. 2018. The role of resveratrol on skeletal muscle cell differentiation and myotube hypertrophy during glucose restriction. Mol Cell Biochem, vol. 444(1-2), 109-123. link> doi> full text>
  • Brown AD, Close GL, Sharples AP, Stewart CE. 2017. Murine myoblast migration: influence of replicative ageing and nutrition. BIOGERONTOLOGY, vol. 18(6), 947-964. link> doi> full text>
  • Fisher A, Seaborne RA, Hughes TM, Gutteridge A, Stewart CE, Coulson JM, Sharples AP, (Joint Corresponding Author), Jarvis JC. 2017. Transcriptomic and Epigenetic Regulation of Disuse Atrophy and the Return to Activity in Skeletal Muscle. FASEB Journal, vol. Epub Ahead of Print. link> doi> link> full text>
  • Allan R, Sharples AP, Close GL, Drust B, Shepherd SO, Dutton J, Morton JP, Gregson W. 2017. Postexercise cold water immersion modulates skeletal muscle PGC-1α mRNA expression in immersed and nonimmersed limbs: evidence of systemic regulation. J Appl Physiol (1985), vol. 123(2), 451-459. link> doi> full text>
  • Sharples AP. 2017. Cellular and Molecular Exercise Physiology: A Historical Perspective for the Discovery of Mechanisms Contributing to Skeletal Muscle Adaptation. Cellular and Molecular Exercise Physiology, vol. 5(1). doi> full text>
  • Kasper AM, Turner DC, Martin NRW, Sharples AP. 2018. Mimicking exercise in three-dimensional bioengineered skeletal muscle to investigate cellular and molecular mechanisms of physiological adaptation. J Cell Physiol, vol. 233(3), 1985-1998. link> doi> full text>
  • Saini A, Sharples AP, Al-Shanti N, Stewart CE. 2017. Omega-3 fatty acid EPA improves regenerative capacity of mouse skeletal muscle cells exposed to saturated fat and inflammation. BIOGERONTOLOGY, vol. 18(1), 109-129. link> doi> full text>
  • Girven M, Dugdale HF, Owens DJ, Hughes DC, Stewart CE, Sharples AP. 2016. L-glutamine Improves Skeletal Muscle Cell Differentiation and Prevents Myotube Atrophy After Cytokine (TNF-alpha) Stress Via Reduced p38 MAPK Signal Transduction. JOURNAL OF CELLULAR PHYSIOLOGY, vol. 231(12), 2720-2732. link> doi> full text>
  • Hammond KM, Impey SG, Currell K, Mitchell N, Shepherd SO, Jeromson S, Hawley JA, Close GL, Hamilton LD, Sharples AP, Morton JP. 2016. Postexercise High-Fat Feeding Suppresses p70S6K1 Activity in Human Skeletal Muscle. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, vol. 48(11), 2108-2117. link> doi> full text>
  • Sharples AP, Stewart CE, Seaborne RA. 2016. Does skeletal muscle have an 'epi'-memory? The role of epigenetics in nutritional programming, metabolic disease, aging and exercise. AGING CELL, vol. 15(4), 603-616. link> doi> full text>
  • Sharples AP, Polydorou I, Hughes DC, Owens DJ, Hughes TM, Stewart CE. 2016. Skeletal muscle cells possess a 'memory' of acute early life TNF-alpha exposure: role of epigenetic adaptation. BIOGERONTOLOGY, vol. 17(3), 603-617. link> doi> full text>
  • Hughes DC, Stewart CE, Sculthorpe N, Dugdale HF, Yousefian F, Lewis MP, Sharples AP. 2016. Testosterone enables growth and hypertrophy in fusion impaired myoblasts that display myotube atrophy: deciphering the role of androgen and IGF-I receptors. BIOGERONTOLOGY, vol. 17(3), 619-639. link> doi> full text>
  • Impey SG, Hammond KM, Shepherd SO, Sharples AP, Stewart C, Limb M, Smith K, Philp A, Jeromson S, Hamilton DL, Morton JP. 2016. Fuel for the work required: A practical approach to amalgamating train-low paradigms for endurance athletes. Physiological Reports, vol. 4(10). doi> full text>
  • Owens DJ, Sharples AP, Donovan T, Tang J, Fraser WD, Morton JP, Stewart CE, Close GL. 2016. Vitamin D and Skeletal Muscle Regeneration: A Systems Approach. Japanese Journal of Physical Fitness and Sports Medicine, vol. 65(1), 157. doi>
  • Owens DJ, Sharples AP, Polydorou I, Alwan N, Donovan T, Tang J, Fraser WD, Cooper RG, Morton JP, Stewart C, Close GL. 2015. A systems-based investigation into vitamin D and skeletal muscle repair, regeneration, and hypertrophy. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, vol. 309(12), E1019-E1031. link> doi>
  • Sharples AP, Hughes DC, Deane CS, Saini A, Selman C, Stewart CE. 2015. Longevity and skeletal muscle mass: the role of IGF signalling, the sirtuins, dietary restriction and protein intake. AGING CELL, vol. 14(4), 511-523. link> doi> full text>
  • Sharples AP and Stewart CE. 2014. Comments on CrossTalk opposing view: The dominant mechanism causing disuse muscle atrophy is proteolysis. The Journal of Physiology, vol. 592(24), 5345-5347. link> doi> link>
  • Player DJ, Martin NRW, Passey SL, Sharples AP, Mudera V, Lewis MP. 2014. Acute mechanical overload increases IGF-I and MMP-9 mRNA in 3D tissue-engineered skeletal muscle (vol 36, pg 1113, 2014). BIOTECHNOLOGY LETTERS, vol. 36(7), 1555-1556. link> doi>
  • Player DJ, Martin NRW, Passey SL, Sharples AP, Mudera V, Lewis MP. 2014. Acute mechanical overload increases IGF-I and MMP-9 mRNA in 3D tissue-engineered skeletal muscle. BIOTECHNOLOGY LETTERS, vol. 36(5), 1113-1124. link> doi>
  • Deane CS, Hughes DC, Sculthorpe N, Lewis MP, Stewart CE, Sharples AP. 2013. Impaired hypertrophy in myoblasts is improved with testosterone administration. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 138, 152-161. link> doi>
  • Deane CS, Hughes DC, Sculthorpe N, Lewis MP, Stewart CE, Sharples AP. 2013. Impaired hypertrophy in myoblasts is improved with testosterone administration. The Journal of Steroid Biochemistry and Molecular Biology, vol. 138(C), 152-161. doi>
  • Martin NRW, Passey SL, Player DJ, Khodabukus A, Ferguson RA, Sharples AP, Mudera V, Baar K, Lewis MP. 2013. Factors affecting the structure and maturation of human tissue engineered skeletal muscle. BIOMATERIALS, vol. 34(23), 5759-5765. link> doi>
  • Sharples AP, Al-Shanti N, Hughes DC, Lewis MP, Stewart CE. 2013. The role of insulin-like-growth factor binding protein 2 (IGFBP2) and phosphatase and tensin homologue (PTEN) in the regulation of myoblast differentiation and hypertrophy. GROWTH HORMONE & IGF RESEARCH, vol. 23(3), 53-61. link> doi>
  • Sharples AP, Player DJ, Martin NRW, Mudera V, Stewart CE, Lewis MP. 2012. Modelling in vivo skeletal muscle ageing in vitro using three-dimensional bioengineered constructs. AGING CELL, vol. 11(6), 986-995. link> doi>
  • Hughes DC, Sculthorpe N, Sharples AP, Lewis MP. 2012. Testosterone and molecular regulation of skeletal muscle. Perspectives on Anabolic Androgenic Steroids (AAS) and Doping in Sport and Health, 27-55.
  • Saini A, Al-Shanti N, Sharples A, Stewart CE. 2012. Sirtuin 1 regulates skeletal myoblast survival and enhances differentiation in the presence of resveratrol. EXPERIMENTAL PHYSIOLOGY, vol. 97(3), 400-418. link> doi>
  • Sharples AP, Al-Shanti N, Lewis MP, Stewart CE. 2011. Reduction of Myoblast Differentiation Following Multiple Population Doublings in Mouse C2C12 Cells: A Model to Investigate Ageing?. JOURNAL OF CELLULAR BIOCHEMISTRY, vol. 112(12), 3773-3785. link> doi>
  • Sharples AP and Stewart CE. 2011. Myoblast models of skeletal muscle hypertrophy and atrophy. CURRENT OPINION IN CLINICAL NUTRITION AND METABOLIC CARE, vol. 14(3), 230-236. link> doi>
  • Sharples AP, Al-Shanti N, Stewart CE. 2010. C-2 and C2C12 Murine Skeletal Myoblast Models of Atrophic and Hypertrophic Potential: Relevance to Disease and Ageing?. JOURNAL OF CELLULAR PHYSIOLOGY, vol. 225(1), 240-250. link> doi>
  • Tolfrey K, Doggett A, Boyd C, Pinner S, Sharples A, Barrett L. 2008. Postprandial triacylglycerol in adolescent boys: A case for moderate exercise. Medicine and Science in Sports and Exercise, vol. 40(6), 1049-1057. doi>
  • Tolfrey K, Doggett A, Boyd C, Pinner S, Sharples A, Barrett L. 2008. Postprandial triacylglycerol in adolescent boys: A case for moderate exercise. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, vol. 40(6), 1049-1057. link> doi>

Chapters

  • Sharples AP, Seaborne RA, Stewart CE. 2017. Epigenetics of skeletal muscle aging. In Epigenetics of Aging and Longevity. Moskalev A and Vaiserman AM (Eds.). (vol. Translational Epigenetics Volume 4). Elsevier. link> doi> link>
  • Hughes D, Sculthorpe N, Sharples AP, Lewis MP. 2012. Testosterone and molecular regulation of skeletal muscle. In: Perspectives on Anabolic Androgenic Steroids (AAS) and Doping in Sport and Health, Nova Science Publishers ISBN 10: 1620812436. In Perspectives on Anabolic Androgenic Steroids (AAS) and Doping in Sport and Health. Grace F and Baker JS (Eds.). Nova Science Publishers. link> link>

Other

  • Allan R and Mawhinney C. 2017. Is the ice bath finally melting? Cold water immersion is no greater than active recovery upon local and systemic inflammatory cellular stress in humans. doi>
  • Dugdale HF, Stewart CE, Sharples AP. 2016. Resveratrol enables myotube survival under nutrient stress. European Muscle Conference. Montpellier, France.
  • Hammond KM, Impey SG, Currell K, Mitchell N, Shepherd SO, Jeromson S, Hawley JA, Close GL, Hamilton LD, Sharples AP, Morton JP. 2016. Postexercise High-Fat Feeding Suppresses p70S6K1 Activity in Human Skeletal Muscle. MEDICINE AND SCIENCE IN SPORTS AND EXERCISE (vol. 48, pp. 2108-2117). link> doi>
  • Allan R, Sharples AP, Close G, Drust B, Shepherd S, Fraser W, Mawhinnney C, Hammond K, Morton J, Gregson W. 2016. The acute cold-induced increase in PGC-1α is partially mediated systemically through increased β-adrenergic stimulation. Vienna, Austria.
  • 2015. Day 1. Free Communications – Physiology and Nutrition. Journal of Sports Sciences (vol. 33, pp. s5-s8). doi>
  • Owens DJ, Polydorou I, Alwan N, Fraser WD, Tang J, Morton J, Sharples AP, Stewart CE, Close GL. 2015. The Role of Vitamin D in Skeletal Muscle Repair: A Systems Biology Approach. Malmo, Sweden.
  • Dugdale HF, Stewart CE, Sharples AP. 2015. The role of SIRT1 in aged and glucose restricted skeletal muscle cells. Proceedings of the Physiological Society. Royal College of Physicians, Edinburgh, United Kingdom. link> link>
  • Sharples AP, Polydorou J, Hughes DC, Hughes TM, Stewart CE. 2015. Skeletal muscle myoblasts have a ‘memory’ of previous TNF-α insults: Role of DNA methylation. Proceedings of the Physiological Society, Accepted. Edinburgh, United Kingdom. link> link>
  • Wong FY, Morris S, Sharples AP, Low DA, Scott MA, Doran DA. 2015. Effect of 30 days oral purine and pyrimidine nucleotide supplementation upon repeated sprint ability performance. Liverpool, UK..
  • Hughes DC, Yousefian F, Sculthorpe N, Stewart CE, Lewis MP, Sharples AP. 2014. Testosterone restores an aged myoblast phenotype via increases in AR and Akt Phosphorylation independently of upstream IGF-IR inhibition. Liverpool, UK..
  • Dugdale HF, Stewart CE, Sharples AP. 2014. The role of SIRTUIN1 in aged and calorie restricted skeletal muscle cells. Liverpool, UK..
  • Sharples AP, Hughes DC, Hughes TM, Stewart CE. 2013. Skeletal muscle myoblasts have a memory of previous TNF-α insults: Role of DNA methylation. Monte Verità, Ascona, Switzerland. link>
  • Potter C, Hughes D, Sharples A, Davies B, Dixon N, Tuttle J, Mauger A, Castle P, Christmas B, McNaughton L. 2013. Combined Effect Of Hyperhydration And Pre-Cooling On Endurance Cycling Performance In Hot And Humid Conditions. Medicine and Science in Sports and Exercise (vol. 5S, pp. 61-71). American College of Sports Medicine. link> link>
  • Deane C, Hughes DC, Sculthorpe N, Sharples AP. 2012. Testosterone rescues differentiation and hypertrophy in artificially aged skeletal muscle myoblasts. Oslo, Norway. link> link>
  • Sharples AP, Player DJ, Martin NRW, Stewart CE, Lewis MP. 2012. Mechanical Stretching of Artificially Aged Myoblasts in Physiologically Relevant 3-Dimensional Bioengineered constructs. Bruge, Belgium.
  • Hughes DC, Sculthorpe N, Sharples AP, Lewis MP. 2012. The effects of testosterone on molecular markers of hypertrophy in C2C12 skeletal muscle cells. Proc Physiol Soc (vol. 26, p. PC61). link> link>
  • Sharples AP, Al-Shanti N, Stewart CE. 2012. Tumour Necrosis Factor- Alpha (TNF-α) increases susceptibility to inhibition of differentiation in artificially aged muscle stem cells. University of Warwick.
  • Player DJ, Martin NRW, Castle PC, Passey S, Sharples AP, Mudera V, Lewis MP. 2011. Mechanical stimulation of 3D bio-engineered skeletal muscle. European Cells and Materials (vol. 22, p. 38).
  • Sharpies AP, Player DJ, Martin NRW, Passey S, Stewart CE, Lewis MP. 2011. Replicative ageing of myoblast in 3-D tissue engineered collagen constructs. European Cells and Materials (vol. 22, p. 39).
  • Player DJ, Martin NRW, Castle PC, Sharples AP, Passey S, Mudera V, Lewis MP. 2011. A putative model of endurance exercise using bio-engineered skeletal muscle. Proc Physiol Soc (vol. 23, p. PC333). link> link>
  • Sharples AP, Player DJ, Martin NRM, Passey S, Stewart CE, Lewis MP. 2011. Artificially aged skeletal myoblasts display reduced regeneration in bio-engineered skeletal muscle. Proc Physiol Soc (vol. 23, p. PC327). link> link>
  • Sharples AP, Al-Shanti N, Stewart CE. 2010. Differential responses of C2 and C2C12 mouse skeletal myoblasts in the absence or presence of tumour necrosis factor- alpha (TNF-α). Endocrine Abstracts (vol. 21, p. P127). Manchester, UK. link> link>

Teaching Expertise

Course Director MSc Cell and Tissue Engineering.

Research Grants Awarded 

  • GlaxoSmithKline, ‘Epi-Memory’ and its role in regeneration and recovery of human skeletal muscle, Dr. Adam Sharples (PI) Prof. Claire Stewart (Co-I) Dr. James Morton (Co-I) Grant value (£): 35,000, Duration of research project: 2 years
  • The Endocrinology Society UK, Equipment Grant: Novel Bioreactor to mimic exercise in in-vitro bioengineered muscle systems, Dr. Adam Sharples (PI), Grant value (£): 5,000, Duration of research project: 1 year
  • LJMU Faculty Studentship (Science/Engineering), Development of a novel optical fibre-based sensor to measure force in human in-vitro skeletal muscle systems., Dr Adam Sharples (DoS), Dr Mark Murphy, Dr. Frederick Bezombes, Grant value (£): 60,000, Duration of research project: 3 years
  • LJMU funded studentship, Novel approaches to the synthesis of multicyclic peptides: Chemical tools to investigate the role of IGF-I in skeletal muscle regeneration and degeneration, PhD Studentship Sanne Verhook, Dr. Chris Coxon (DoS), Dr. Adam Sharples (Supervisor), Professor Claire Stewart (Supervisor), Grant value (£): £57,867, Duration of research project: 3 years,
  • LJMU, 'Epigenetic regulation of skeletal muscle mass and performance: Does muscle have an ‘Epi-memory.’ PhD studentship: Robert Seaborne, Dr. Adam Sharples (DoS), Professor Claire Stewart, Dr. James Morton, Dr. Chris Murgatroyd, Grant value (£): £57,867, Duration of research project: 3 years,
  • GlaxoSmithKline (GSK) and LJMU, Post-damage Repair and Regeneration of Skeletal Muscle: Underlying Mechanisms? PhD Studentship Alex Brown, Professor Claire Stewart, Dr. Adam Sharples (Supervisor), Dr. Graeme Close (Supervisor), Grant value (£): £59,000 (30K GSK and 29K LJMU), Duration of research project: 3 years
  • Rugby Football Union (RFU) and LJMU, 'Personalising elite training through the use of individualised in-vitro model systems to simulate exercise.' PhD studentship Andreas Kasper, Dr. Adam Sharples (DoS), Dr. G. Close, Prof. Claire Stewart, Grant value (£): £67,500 (37.5K RFU, 30K LJMU), Duration of research project: 3 years.
  • Malaysian Government, Nucleotide supplementation in humans and high intensity repeated sprint exercise performance, muscle strength, damage and oxygen transport. PhD Studentship Fui Yen Wong, Dr. Dominic Doran (Dos), Dr. Adam Sharples (Supervisor), Dr. David Low (Supervisor), Grant value (£): 80,000, Duration of research project: 3 years
  • English Institute of Sport, Nutritional modulations of exercise-induced alterations in skeletal muscle oxidative capacity. PhD Studentship Kelly Hammond, Dr. Morton (PI), Dr. Close (supervisor), Dr Sharples (supervisor), Grant value (£): 30,000 EIS plus approx 28,000 (LJMU matched funding), Duration of research project: 3 years
  • Malaysian Government, Influence of training load on training responses and adaptation. PhD Studentship- Mohd Firdaus Bin Maasar, Prof Drust (DoS), Dr. Andrew Hulton (supervisor) Dr. Sharples (supervisor), Grant value (£): 67,616.00, Duration of research project: 3 years
  • The Physiological Society (Physoc) & LJMU, Does calorie restriction induce muscle wasting with age? Epigenetics and the cellular and molecular role of the Sirtuins, Dr. Adam Sharples, Grant value (£): £23,732 (10K Physoc, 13.7K LJMU), Duration of research project: 3 years