Prof Paul Eggleston

Title: Professor Emeritus of Molecular Entomology
Phone:
Email: p.eggleston@keele.ac.uk
Location:
Role: Retired
Contacting me:
paul eggleston

I was appointed to the Chair of Molecular Entomology in the Centre for Applied Entomology and Parasitology (now part of the Institute for Science and Engineering in Medicine) in 1999. Before that, I obtained a B.Sc. (Hons.) in Biological Sciences from Birmingham University in 1977 and a Ph.D. in Genetics, also from Birmingham University, in 1981. Shortly afterwards, I was appointed to a Lectureship in the Department of Genetics and Microbiology at the University of Liverpool, progressing to a Senior Lectureship in Genetics within the new School of Biological Sciences at Liverpool University in 1993. During this time, I was awarded a Senior Research Fellowship from the Lister Institute of Preventive Medicine, which was held at the Liverpool School of Tropical Medicine from 1987-1995. I was also elected to present the 1990 Balfour Lecture to the Genetics Society, an award that recognizes outstanding achievement by a young Geneticist.

My research interests are in molecular entomology, particularly the molecular genetics of mosquitoes that transmit human disease and their complex interactions with the parasites and viruses that cause disease. Because of their medical importance, the focus of my group is on the malaria vector mosquito, Anopheles gambiae and the yellow fever mosquito, Aedes aegypti. Current projects include the development of technologies for genetic engineering of mosquitoes, the creation of genetically modified mosquitoes that are compromised in their ability to transmit disease and the development of strategies for stage- and tissue-specific gene expression within genetically modified mosquitoes. My research has attracted external funding of just under £4 million since 1982 and this has resulted in over 55 primary publications and 65 published abstracts. I am currently a Fellow of the Royal Entomological Society and a member of the Lister Institute of Preventive Medicine, the British Society for Parasitology and the Genetics Society.

Further details

Selected Publications

  • Eggleston P and Meredith JM. 2014. Docking systems for site-directed transgene integration. In Transgenic Insects: Techniques and Applications. Benedict MQ (Ed.). (22 vols.). CABI Press.
  • Pondeville E, Puchot N, Meredith JM, Lynd A, Vernick KD, Lycett GJ, Eggleston P, Bourgouin C. 2014. Efficient Phi C31 integrase-mediated site-specific germline transformation of Anopheles gambiae. NATURE PROTOCOLS, vol. 9(7), 1698-1712. link> doi>
  • McArthur CC, Meredith JM, Eggleston P. 2014. Transgenic Anopheles gambiae expressing the synthetic Vida3 antimalarial peptide suffer no significant fitness cost. PLoS One, vol. 9(2). doi>
  • Pondeville E, Puchot N, Eggleston P, Lycett G, Bourgouin C. 2013. DEVELOPMENT OF THE GAL4/UAS SYSTEM IN ANOPHELES GAMBIAE FOR FUNCTIONAL ANALYSIS OF PLASMODIUM - ANOPHELES INTERACTIONS. PATHOGENS AND GLOBAL HEALTH, vol. 107(8), 444. link>
  • Paton D, Toure M, Sacko A, Underhill A, Meredith J, Eggleston P, Coulibaly M, Traore C, Tripet F. 2013. TOWARDS THE RELEASE OF TRANSGENIC MOSQUITOES: FITNESS AND MATING COMPETITIVENESS IN TRANSGENIC AND LABORATORY STRAINS OF ANOPHELES GAMBIAE SENSU STRICTO. PATHOGENS AND GLOBAL HEALTH, vol. 107(8), 420. link>

Full Publications List show

Books

  • EGGLESTON P. EGGLESTON P and Crampton JM (Eds.). 1992. Insect Molecular Science. Academic Press, London..

Journal Articles

  • Pondeville E, Puchot N, Meredith JM, Lynd A, Vernick KD, Lycett GJ, Eggleston P, Bourgouin C. 2014. Efficient Phi C31 integrase-mediated site-specific germline transformation of Anopheles gambiae. NATURE PROTOCOLS, vol. 9(7), 1698-1712. link> doi>
  • McArthur CC, Meredith JM, Eggleston P. 2014. Transgenic Anopheles gambiae expressing the synthetic Vida3 antimalarial peptide suffer no significant fitness cost. PLoS One, vol. 9(2). doi>
  • Pondeville E, Puchot N, Eggleston P, Lycett G, Bourgouin C. 2013. DEVELOPMENT OF THE GAL4/UAS SYSTEM IN ANOPHELES GAMBIAE FOR FUNCTIONAL ANALYSIS OF PLASMODIUM - ANOPHELES INTERACTIONS. PATHOGENS AND GLOBAL HEALTH, vol. 107(8), 444. link>
  • Paton D, Toure M, Sacko A, Underhill A, Meredith J, Eggleston P, Coulibaly M, Traore C, Tripet F. 2013. TOWARDS THE RELEASE OF TRANSGENIC MOSQUITOES: FITNESS AND MATING COMPETITIVENESS IN TRANSGENIC AND LABORATORY STRAINS OF ANOPHELES GAMBIAE SENSU STRICTO. PATHOGENS AND GLOBAL HEALTH, vol. 107(8), 420. link>
  • Carter V, Underhill A, Baber I, Sylla L, Baby M, Larget-Thiery I, Zettor A, Bourgouin C, Langel U, Faye I, Otvos L, Wade JD, Coulibaly MB, Traore SF, Tripet F, Eggleston P, Hurd H. 2013. Killer Bee Molecules: Antimicrobial Peptides as Effector Molecules to Target Sporogonic Stages of Plasmodium. PLoS Pathogens. doi>
  • Meredith JM, Underhill A, McArthur CC, Eggleston P. 2013. Next-Generation Site-Directed Transgenesis in the Malaria Vector Mosquito Anopheles gambiae: Self-Docking Strains Expressing Germline-Specific phiC31 Integrase. PLoS One, vol. 8(3). doi>
  • Paton D, Underhill A, Meredith J, Eggleston P, Tripet F. 2013. Contrasted fitness costs of docking and antibacterial constructs in the EE and EVida3 strains validates two-phase Anopheles gambiae genetic transformation system. PLoS One. doi>
  • Meredith JM, Basu S, Nimmo DD, Larget-Thiery I, Warr EL, Underhill A, McArthur CC, Carter V, Hurd H, Bourgouin C, Eggleston P. 2011. Site-specific integration and expression of an anti-malarial gene in transgenic Anopheles gambiae significantly reduces Plasmodium infections. PLoS One, vol. 6(1), e14587. link> doi>
  • Meredith JM, Hurd H, Lehane MJ, Eggleston P. 2008. The malaria vector mosquito Anopheles gambiae expresses a suite of larval-specific defensin genes. Insect Mol Biol, vol. 17(2), 103-112. link> doi>
  • Sethuraman N, Jr FMJ, Eggleston P, O'Brochta DA. 2007. Post-integration stability of piggy Bac in Aedes aegypti. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 37(9), 941-951. link> doi>
  • Meredith JM, Munks RJL, Grail W, Hurd H, Eggleston P, Lehane MJ. 2006. A novel association between clustered NF-kappa B and C/EBP binding sites is required for immune regulation of mosquito Defensin genes. INSECT MOLECULAR BIOLOGY, vol. 15(4), 393-401. link> doi>
  • Warr E, Meredith JM, Nimmo DD, Basu S, Hurd H, Eggleston P. 2006. A tapeworm molecule manipulates vitellogenin expression in the beetle Tenebrio molitor. Insect Mol Biol, vol. 15(4), 497-505. link> doi>
  • Nimmo DD, Alphey L, Meredith JM, Eggleston P. 2006. High efficiency site-specific genetic engineering of the mosquito genome. INSECT MOLECULAR BIOLOGY, vol. 15(2), 129-136. link> doi>
  • Hurd H, Taylor PJ, Adams D, Underhill A, Eggleston P. 2005. Evaluating the costs of mosquito resistance to malaria parasites. Evolution, vol. 59(12), 2560-2572. link> doi>
  • Ding YC, Hawkes N, Meredith J, Eggleston P, Hemingway J, Ranson H. 2005. Characterization of the promoters of Epsilon glutathione transferases in the mosquito Anopheles gambiae and their response to oxidative stress. BIOCHEMICAL JOURNAL, vol. 387, 879-888. link> doi>
  • Warr E, Eggleston P, Hurd H. 2004. Apoptosis in the fat body tissue of the beetle Tenebrio molitor parasitised by Hymenolepis diminuta. J Insect Physiol, vol. 50(11), 1037-1043. link> doi>
  • Cole TJ, Eggleston P, Hurd H. 2003. Juvenile hormone titre and egg production in Tenebrio molitor infected by Hymenolepis diminuta: effect of male and/or female infection, male age and mating. J Insect Physiol, vol. 49(6), 583-590. link> doi>
  • Alphey L, Beard B, Billingsley P, Coetzee M, Cristanti A, Curtis C, Eggleston P, Godfray C, Hemingway J, Jacobs-Lorena M, James A, Kafatos F, Mukwaya L, Paton M, Powell J, Schneider W, Scott T, Sina B, Sinden R, Sinkins S, et A. 2002. Malaria control with genetically manipulated insect vectors. Science, vol. 298(4 Oct 02), 119-121. doi>
  • Zhang HJ, Shinmyo Y, Hirose A, Mito T, Inoue Y, Ohuchi H, Loukeris TG, Eggleston P, Noji S. 2002. Extrachromosomal transposition of the transposable element Minos in embryos of the cricket Gryllus bimaculatus. DEVELOPMENT GROWTH & DIFFERENTIATION, vol. 44(5), 409-417. link> doi>
  • Eggleston P and Zhao Y. 2001. A sensitive and rapid assay for homologous recombination in mosquito cells: impact of vector topology and implications for gene targeting. BMC Genet, vol. 2, 21. link> doi>
  • Eggleston P and Zhao Y. 2001. Gene targeting in mosquito cells: a demonstration of 'knockout' technology in extrachromosomal gene arrays. BMC Genet, vol. 2, 11. link> doi>
  • Devenport MP, Blass C, Eggleston P. 2000. Characterization of the Hox gene cluster in the malaria vector mosquito, Anopheles gambiae. EVOLUTION & DEVELOPMENT, vol. 2(6), 326-339. link> doi>
  • Eggleston P, Lu W, Zhao Y. 2000. Genomic organization and immune regulation of the defensin gene from the mosquito, Anopheles gambiae. Insect Mol Biol, vol. 9(5), 481-490. link> doi>
  • Zhao YG and Eggleston P. 1999. Comparative analysis of promoters for transient gene expression in cultured mosquito cells. Insect Mol Biol, vol. 8(1), 31-38. link> doi>
  • Zhao YG and Eggleston P. 1998. Stable transformation of an Anopheles gambiae cell line mediated by the Hermes mobile genetic element. INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, vol. 28(4), 213-219. link> doi>
  • Eggleston P and Spiers S. 1995. Characterization of a transmembrane receptor PTPase expressed during CNS development in the mosquito Anopheles gambiae. Journal of Cellular Biochemistry, vol. 59(Supplement 21A), 216.
  • Zhao Y and Eggleston P. 1995. DNA transformation in the mosquito: the potential of homologous recombination. Journal of Cellular Biochemistry, vol. 59(Supplement 21A), 229.
  • King AN, Crampton JM, Eggleston P. 1995. Stable transformation of the mosquitoes Anopheles gambiae and Aedes aegypti. Journal of Cellular Biochemistry, vol. 59(Supplement 21A), 224.
  • Bacon RE and Eggleston P. 1995. Structure and expression of the homeotic gene abdominal-A in the mosquito Aedes aegypti. Journal of Cellular Biochemistry, vol. 59(Supplement 21A), 214.
  • Oduol FO, Crampton JM, Eggleston P. 1995. Structure, organization and expression of trypsin genes in the mosquito Aedes aegypti. Journal of Cellular Biochemistry, vol. 59(Supplement 21A), 210.
  • Crampton JM, Warren A, Lycett GJ, Hughes MA, Comley IP, Eggleston P. 1994. Genetic manipulation of insect vectors as a strategy for the control of vector-borne disease. Ann Trop Med Parasitol, vol. 88(1), 3-12. link> doi>
  • Oduol FO, Crampton JM, Eggleston P. 1994. Isolation and characterization of trypsin genes from Aedes aegypti and Anopheles gambiae using PCR amplification. Transactions of The Royal Society of Tropical Medicine and Hygiene, vol. 88, 277-284.
  • Crampton JM, Comley I, Eggleston P, Hill S, Hughes M, Knapp T, Lycett G, Urwin R, Warren A. 1992. Molecular biological approaches to the study of vectors in relation to malaria control. Mem Inst Oswaldo Cruz, vol. 87 Suppl 3, 43-49. link> doi>
  • Eggleston P and Exley KA. 1992. Chromosome interactions in P-M dysgenic male recombination of Drosophila melanogaster. Genet Res, vol. 60(3), 165-174. link>
  • Eggleston P. 1992. Identification of the abdominal-A homologue from Aedes aegypti and structural comparisons among related genes. Nucleic Acids Res, vol. 20(15), 4095. link> doi>
  • De Miranda JR, Hemmat M, Eggleston P. 1991. The competition diallel and the exploitation and interference components of larval competition in Drosophila melanogaster. Heredity (Edinb), vol. 66 ( Pt 3), 333-342. link> doi>
  • Eggleston P. 1991. The 1990 Balfour lecture. The control of insect-borne disease through recombinant DNA technology. Heredity (Edinb), vol. 66 ( Pt 2), 161-172. link> doi>
  • Hemmat M and Eggleston P. 1990. The biometrical genetics of competitive parameters in Drosophila melanogaster. Heredity (Edinb), vol. 64 ( Pt 2), 223-231. link> doi>
  • Hemmat M and Eggleston P. 1990. Analysis of competitive interactions in triocultures of Drosophila melanogaster. Heredity (Edinb), vol. 64 ( Pt 2), 215-222. link> doi>
  • Crampton J, Morris A, Lycett G, Warren A, Eggleston P. 1990. Transgenic mosquitoes: a future vector control strategy?. Parasitol Today, vol. 6(2), 31-36. link> doi>
  • de Miranda JR and Eggleston P. 1989. Analysis of dominance for competitive ability in Drosophila melanogaster. Heredity (Edinb), vol. 63 ( Pt 2), 221-229. link> doi>
  • EXLEY KA and EGGLESTON P. 1989. STRUCTURE, FREQUENCY AND DISTRIBUTION OF P-ELEMENTS IN RELATION TO P M HYBRID DYSGENIC MALE RECOMBINATION IN DROSOPHILA-MELANOGASTER. GENETICAL RESEARCH, vol. 53(3), 163-171. link> doi>
  • Morris AC, Eggleston P, Crampton JM. 1989. Genetic transformation of the mosquito Aedes aegypti by micro-injection of DNA. Med Vet Entomol, vol. 3(1), 1-7. link> doi>
  • de Miranda JR and Eggleston P. 1988. Genetic analysis of larval competition in Drosophila melanogaster. Heredity (Edinb), vol. 61 ( Pt 3), 339-346. link> doi>
  • Hemmat M and Eggleston P. 1988. Competitive interactions in Drosophila melanogaster: genetic variation for interference through media conditioning. Heredity (Edinb), vol. 61 ( Pt 3), 347-354. link> doi>
  • de Miranda JR and Eggleston P. 1988. Larval competition in Drosophila melanogaster. I. Estimation of larval growth parameters. Heredity (Edinb), vol. 60 ( Pt 2), 205-212. link> doi>
  • de Miranda JR and Eggleston P. 1988. Larval competition in Drosophila melanogaster. II. Comparing biological and competitive parameters. Heredity (Edinb), vol. 60 ( Pt 2), 213-219. link> doi>
  • Hemmat M and Eggleston P. 1988. Competitive interactions in Drosophila melanogaster: recurrent selection for aggression and response. Heredity (Edinb), vol. 60 ( Pt 1), 129-137. link> doi>
  • DEMIRANDA JR and EGGLESTON P. 1987. A COMPARISON OF SUBSTITUTION AND ADDITION DESIGNS FOR THE ANALYSIS OF COMPETITIVE INTERACTIONS IN DROSOPHILA-MELANOGASTER. HEREDITY, vol. 58, 279-288. link> doi>
  • Eggleston P. 1987. The use of genetic markers in the analysis of competitive interactions in Drosophila melanogaster. Genetica, vol. 72(3), 181-186. link> doi>
  • Rodrigues de Miranda J and Eggleston P. 1986. Substitution and addition designs for measuring aggression and response. Eucarpia, vol. 6, 237-242.
  • EGGLESTON P. 1985. VARIATION FOR AGGRESSION AND RESPONSE IN THE COMPETITIVE INTERACTIONS OF DROSOPHILA-MELANOGASTER. HEREDITY, vol. 54(FEB), 43-51. link> doi>
  • Eggleston P. 1984. Correlation in the induction and response of SF and GD sterility. Drosophila Information Service, vol. 60, 99-101.
  • EGGLESTON P. 1984. HYBRID DYSGENESIS IN DROSOPHILA-MELANOGASTER - THE FREQUENCY AND DISTRIBUTION OF MALE RECOMBINATION EVENTS. HEREDITY, vol. 52(APR), 189-202. link> doi>
  • Eggleston P and Kearsey MJ. Hybrid dysgenesis in Drosophila: Correlation between dysgenic traits. Heredity, 237-249. doi>

Chapters

  • Eggleston P and Meredith JM. 2014. Docking systems for site-directed transgene integration. In Transgenic Insects: Techniques and Applications. Benedict MQ (Ed.). (22 vols.). CABI Press.
  • Crampton JM and Eggleston P. 2006. Transgenic Insects. In Animals With Novel Genes. Maclean N (Ed.). (7 vols.). Cambridge University Press.
  • Eggleston P and Carlson J. 2002. Summary of the workshop on DNA transformation of non-drosophilid insects. In Insect Molecular Science: 16th Symposium of the Royal Entomological Society of London. Crampton JM and Eggleston P (Eds.). Academic Press.
  • Eggleston P and Carlson J. 2002. Summary of the workshop on DNA transformation of non-drosophilid insects. In Insect Molecular Science: 16th Symposium of the Royal Entomological Society of London. Crampton JM and Eggleston P (Eds.). Academic Press.
  • Eggleston P and Zhao Y. 2000. Targeted transformation of the insect genome. In Insect Transgenesis: Methods and Applications. Handler AM and James AA (Eds.). (19 vols.). Boca Raton: CRC Press.
  • Eggleston P. 1992. Biotechnology and the control of mosquitoes. In Animal Parasite Control Utilising Biotechnology. Yong WK (Ed.). CRC Press.
  • Crampton JM, Morris AC, Lycett GJ, Warren AM, Eggleston P. 1990. Molecular characterisation and genome manipulation of the mosquito Aedes aegypti. In Molecular Insect Science. Hagedorn HH, Hildebrand JG, Kidwell MG, Law JH (Eds.). Plenum Press.

Other

  • Eggleston P, Basu S, Meredith J, Medimond. 2006. High efficiency site-directed genetic engineering of the mosquito genome. ICOPA XI: PROCEEDINGS OF THE 11TH INTERNATIONAL CONGRESS OF PARASITOLOGY (pp. 71-+). link>
  • Eggleston P. 1981. Hybrid dysgenesis in Drosophila melanogaster.
  • LSC-10032 Genetics and Evolution (Module Manager)

  • LSC-10031 Cell and Molecular Biology (Module Manager)

  • LSC-30007 Dissertation for Biology

  • LSC-30013 Biology Research Project(non-experimental)

  • LSC-30014 Biochemistry Research Project (non-experimental)

  • LSC-30015 Biology of Disease

  • LSC-30022 Non-experimental Project

  • LSC-30023 Dissertation M.Sc. (Molecular Parasitology and Vector Biology)