Dr Dan Tonge
- Title
- Lecturer in Biosciences
- Location
- Huxley Building : 170
- Contact me
- Try my office or arrange an appointment by e-mail.
- Phone
- +44 (0)1782 733418
- d.p.tonge@keele.ac.uk
Biography
Dr Daniel Tonge graduated with a BSc(Hons) in Biomedical Sciences incorporating a year-long clinical training placement in histopathology and cytology at the Royal Liverpool and Broadgreen Hospital. Following graduation, he completed a PhD in Molecular Biology at the University of Nottingham with funding from AstraZeneca and Arthritis Research UK.
His doctoral thesis investigated molecular changes in the quadriceps skeletal muscle in various experimental models of knee osteoarthritis. After completing his doctoral studies, Dr Tonge undertook post-doctoral research positions at the School of Veterinary Medicine, Nottingham (“molecular control of skeletal muscle fibre type”), the Medical Research Council (“next generation sequencing of small non-coding RNAs for biomarker discovery”) and the Health Protection Agency, Oxford.
In 2012, he was appointed as a Senior Scientist at the Health Protection Agency (Public Health England thereafter) with responsibility for a range of research projects covering nucleic acid biomarker discovery and next generation sequencing-driven fungal metagenomics. Whilst at PHE, Dr Tonge was responsible for the implementation and management of a local next generation sequencing facility and was a member of the agency-wide next generation sequencing and bioinformatics working group. In May 2015, Dr Tonge joined Keele University as a Lecturer in Biosciences.
Research and scholarship
Dr Tonge's current research involves the application (and development) of next generation sequencing and bioinformatic techniques to the areas of personalised medicine (key disease pathway analysis and novel biomarker discovery) and microbial metagenomics.
Current projects include;
- An analysis of the circulating miRNA profile of severe, poorly controlled asthmatic patients
- The role of obesity in the pathogenesis of hip osteoarthritis – elucidation of key nucleic acid signatures
- An analysis of circulating microbial nucleic acids in hip osteoarthritis patients
- Metagenomic analyses of the rhizosphere microbiome and its impact on VOC detoxification (PhD Studentship)
Although I work in all disease areas, I have a particular interest in the pathogenesis of Osteoarthritis and the potential for the development of personalised / precision therapeutics.
Selected Publications
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2019. Molecular Characterization of Circulating Microbiome Signatures in Rheumatoid Arthritis. Front Cell Infect Microbiol, 440, vol. 9. link> doi> full text>
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2019. Multi-Method Molecular Characterisation of Human Dust-Mite-associated Allergic Asthma. Scientific Reports, Article 8912, vol. 9(1). link> doi> link> full text>
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2019. RNA sequencing reveals a key role for the long non-coding RNA MIAT in regulating neuroblastoma and glioblastoma cell fate. Int J Biol Macromol, 878-891, vol. 130. link> doi> full text>
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2018. Multi-Method Characterization of the Human Circulating Microbiome. Front Microbiol, 3266, vol. 9. link> doi> full text>
Full Publications Listshow
Journal Articles
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2019. Molecular Characterization of Circulating Microbiome Signatures in Rheumatoid Arthritis. Front Cell Infect Microbiol, 440, vol. 9. link> doi> full text>
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2019. Multi-Method Molecular Characterisation of Human Dust-Mite-associated Allergic Asthma. Scientific Reports, Article 8912, vol. 9(1). link> doi> link> full text>
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2019. RNA sequencing reveals a key role for the long non-coding RNA MIAT in regulating neuroblastoma and glioblastoma cell fate. Int J Biol Macromol, 878-891, vol. 130. link> doi> full text>
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2018. Multi-Method Characterization of the Human Circulating Microbiome. Front Microbiol, 3266, vol. 9. link> doi> full text>
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2016. What is normal? Next generation sequencing-driven analysis of the human circulating miRNAOme. BMC Mol Biol, 4, vol. 17. link> doi> full text>
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2014. Amplicon -based Metagenomic Analysis of Mixed Fungal Samples using Proton Release Amplicon Sequencing. PLoS One. doi> full text>
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2013. Evidence of Changes to Skeletal Muscle Contractile Properties During the Initiation of Disease in the Ageing Guinea Pig Model of Osteoarthritis. Longevity and Healthspan. doi>
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Characterisation of the sarcomeric myosin heavy chain multigene family in the laboratory guinea pig. BMC Molecular Biology. doi>
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Evidence Based Housekeeping Gene Selection for microRNA-sequencing (miRNA-seq) Studies. Toxicological Research. doi>
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The role of microRNAs in the pathogenesis of MMPi-induced skin fibrodysplasia. BMC Genomics. doi>
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β2-Adrenergic agonist-induced hypertrophy of the quadriceps skeletal muscle does not modulate disease severity in the rodent meniscectomy model of osteoarthritis. Osteoarthritis and Cartilage. doi>
Other
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2018. Identification of differentially expressed lncrnas in metformin resistant SH-SY5Y neuroblastoma cells. ESMO Open. BMJ Journals. link> doi> full text>
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2014. Global miRNA profiling for biomarker discovery – What is normal?. Toxicology Letters. Elsevier. doi>
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2012. The application of Next Generation Sequencing to the detection and analysis of miRNAs. Toxicology Letters. Elsevier. doi>