Annette Shrive

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Biography

I undertook both my BSc (Physics and Maths) and PhD (Protein Crystallography) at Keele University. The latter involved the development of synchrotron x-ray Laue method for use with large macromolecules such as proteins and viruses. I revisited Keele as a postdoctoral worker and was appointed as a Lecturer at Keele University in September 1999. I have been a visiting scientist at Purdue University, USA and LMB Cambridge and I have undertaken postdoctoral work in structural biology at Keele University, University of Edinburgh and Purdue University, USA.

Research and scholarship

My research interests are in structural biology and in how the structure of biological macromolecules is related to their function and is focused on the structure and function of key molecules of the innate immune system. The innate immune response has non-variable components which respond in a fairly general, often rapid manner to threatening microorganisms and molecules of both internal and external origin. A major focus of my research is on the three-dimensional structures of pentraxins and active fragments of collectins and related proteins and their interactions with natural ligands. Alongside structural work, we also aim to understand how these molecules are functioning in the immune system. From a clinical perspective, one aspect of this work includes analysis of possible modified forms of C-reactive protein in patient serum samples (REC reference 17/WM/0150). Further information on the work of the Structural Biology Research group can be found here.

Human surfactant protein D

FIBCD1 with bound ligand

Human C-reactive protein - Image - T J Greenhough & A K Shrive © Keele University

Teaching

Year 1

LSC-10070 Clinical Applications of Biomedical Science

Year 2

LSC-20015 Molecular, Cellular & Structural Immunology (Module manager)

Year 3

LSC-30015 Biology of Disease (Module manager)
LSC-30016 Structural Biology & Macromolecular Function
LSC-30026 Case Studies in Biomedical Sciences
LSC-30045 Life Sciences Double Experimental Project
LSC-30050 Life Sciences Dissertation

Selected Publications

Williams RD, Moran JA, Fryer AA, Littlejohn JR, Williams HM, Greenhough TJ, Shrive AK. 2020. Monomeric C-Reactive Protein in Serum With Markedly Elevated CRP Levels Shares Common Calcium-Dependent Ligand Binding Properties With an in vitro Dissociated Form of C-Reactive Protein. Frontiers in Immunology, vol. 11. doi> link> full text>
Paterson JM, Shaw AJ, Burns I, Dodds AW, Prasad A, Reid KB, Greenhough TJ, Shrive AK. 2019. Atomic-resolution crystal structures of the immune protein conglutinin from cow reveal specific interactions of its binding site with N-acetylglucosamine. J Biol Chem, 17155-17165, vol. 294(45). link> doi> full text>
Littlejohn JR, da Silva RF, Neale WA, Smallcombe CC, Clark HW, Mackay R-MA, Watson AS, Madsen J, Hood DW, Burns I, Greenhough TJ, Shrive AK. 2018. Structural definition of hSP-D recognition of Salmonella enterica LPS inner core oligosaccharides reveals alternative binding modes for the same LPS. PLoS One, e0199175, vol. 13(6). link> doi> full text>
Clark HW, Mackay R-M, Deadman ME, Hood DW, Madsen J, Moxon ER, Townsend JP, Reid KBM, Ahmed A, Shaw AJ, Greenhough TJ, Shrive AK. 2016. Crystal Structure of a Complex of Surfactant Protein D (SP-D) and Haemophilus influenzae Lipopolysaccharide Reveals Shielding of Core Structures in SP-D-Resistant Strains. Infect Immun, 1585-1592, vol. 84(5). link> doi> full text>
Pionnier N, Falco A, Miest JJ, Shrive AK, Hoole D. 2014. Feeding common carp Cyprinus carpio with β-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection. Fish Shellfish Immunol, 285-295, vol. 39(2). link> doi>

Full Publications Listshow

Journal Articles

Williams RD, Moran JA, Fryer AA, Littlejohn JR, Williams HM, Greenhough TJ, Shrive AK. 2020. Monomeric C-Reactive Protein in Serum With Markedly Elevated CRP Levels Shares Common Calcium-Dependent Ligand Binding Properties With an in vitro Dissociated Form of C-Reactive Protein. Frontiers in Immunology, vol. 11. doi> link> full text>
Paterson JM, Shaw AJ, Burns I, Dodds AW, Prasad A, Reid KB, Greenhough TJ, Shrive AK. 2019. Atomic-resolution crystal structures of the immune protein conglutinin from cow reveal specific interactions of its binding site with N-acetylglucosamine. J Biol Chem, 17155-17165, vol. 294(45). link> doi> full text>
Littlejohn JR, da Silva RF, Neale WA, Smallcombe CC, Clark HW, Mackay R-MA, Watson AS, Madsen J, Hood DW, Burns I, Greenhough TJ, Shrive AK. 2018. Structural definition of hSP-D recognition of Salmonella enterica LPS inner core oligosaccharides reveals alternative binding modes for the same LPS. PLoS One, e0199175, vol. 13(6). link> doi> full text>
Clark HW, Mackay R-M, Deadman ME, Hood DW, Madsen J, Moxon ER, Townsend JP, Reid KBM, Ahmed A, Shaw AJ, Greenhough TJ, Shrive AK. 2016. Crystal Structure of a Complex of Surfactant Protein D (SP-D) and Haemophilus influenzae Lipopolysaccharide Reveals Shielding of Core Structures in SP-D-Resistant Strains. Infect Immun, 1585-1592, vol. 84(5). link> doi> full text>
Pionnier N, Falco A, Miest JJ, Shrive AK, Hoole D. 2014. Feeding common carp Cyprinus carpio with β-glucan supplemented diet stimulates C-reactive protein and complement immune acute phase responses following PAMPs injection. Fish Shellfish Immunol, 285-295, vol. 39(2). link> doi>
Shrive AK, Moeller JB, Burns I, Paterson JM, Shaw AJ, Schlosser A, Sorensen GL, Greenhough TJ, Holmskov U. 2014. Crystal structure of the tetrameric fibrinogen-like recognition domain of fibrinogen C domain containing 1 (FIBCD1) protein. J Biol Chem, 2880-2887, vol. 289(5). link> doi>
Pionnier N, Falco A, Miest J, Frost P, Irnazarow I, Shrive A, Hoole D. 2013. Dietary β-glucan stimulate complement and C-reactive protein acute phase responses in common carp (Cyprinus carpio) during an Aeromonas salmonicida infection. Fish Shellfish Immunol, 819-831, vol. 34(3). link> doi>
Mold M, Shrive AK, Exley C. 2012. Serum amyloid P component accelerates the formation and enhances the stability of amyloid fibrils in a physiologically significant under-saturated solution of amyloid-β42. J Alzheimers Dis, 875-881, vol. 29(4). link> doi>
Shrive AK, Martin C, Burns I, Paterson JM, Martin JD, Townsend JP, Waters P, Clark HW, Kishore U, Reid KBM, Greenhough TJ. 2009. Structural characterisation of ligand-binding determinants in human lung surfactant protein D: influence of Asp325. J Mol Biol, 776-788, vol. 394(4). link> doi>
Shrive AK, Burns I, Chou H-T, Stahlberg H, Armstrong PB, Greenhough TJ. 2009. Crystal structures of Limulus SAP-like pentraxin reveal two molecular aggregations. J Mol Biol, 1240-1254, vol. 386(5). link> doi>
MacCarthy EM, Burns I, Irnazarow I, Polwart A, Greenhough TJ, Shrive AK, Hoole D. 2008. Serum CRP-like protein profile in common carp Cyprinus carpio challenged with Aeromonas hydrophila and Escherichia coli lipopolysaccharide. Dev Comp Immunol, 1281-1289, vol. 32(11). link> doi>
Kishore U, Greenhough TJ, Waters P, Shrive AK, Ghai R, Kamran MF, Bernal AL, Reid KBM, Madan T, Chakraborty T. 2006. Surfactant proteins SP-A and SP-D: structure, function and receptors. Mol Immunol, 1293-1315, vol. 43(9). link> doi>
Kishore U, Ghai R, Greenhough TJ, Shrive AK, Bonifati DM, Gadjeva MG, Waters P, Kojouharova MS, Chakraborty T, Agrawal A. 2005. Structural and functional anatomy of the globular domain of complement protein C1q (vol 95, pg 113, 2004). IMMUNOLOGY LETTERS, II, vol. 101(1). link> doi>
GREENHOUGH TJ, Gaboriaud C, Kishore U, Waters P. 2004. C1q and tumor necrosis factor superfamily: modularity and versatility. Trends in Immunology, 551-561, vol. 25(10). doi>
Kishore U, Ghai R, Greenhough TJ, Shrive AK, Bonifati DM, Gadjeva MG, Waters P, Kojouharova MS, Chakraborty T, Agrawal A. 2004. Structural and functional anatomy of the globular domain of complement protein Clq. Immunology Letters, 113-128, vol. 95(2). doi>
Cartwright JR, Tharia HA, Burns I, Shrive AK, Hoole D, Greenhough TJ. 2004. Isolation and characterisation of pentraxin-like serum proteins from the common carp Cyprinus carpio. Dev Comp Immunol, 113-125, vol. 28(2). link> doi>
Cartwright JR, Tharia HA, Burns I, Shrive AK, Hoole D, Greenhough TJ. 2004. Isolation and characterization of pentraxin-like serum proteins from the common carp \fICyprinus carpio\fP. Developmental and Comparative Immunology.
Shrive AK, Tharia HA, Strong P, Kishore U, Burns I, Rizkallah PJ, Reid KBM, Greenhough TJ. 2003. High-resolution structural insights into ligand binding and immune cell recognition by human lung surfactant protein D. J Mol Biol, 509-523, vol. 331(2). link> doi>
Lee WH, Perles LA, Nagem RAP, Shrive AK, Hawkins A, Sawyer L, Polikarpov I. 2003. Comparison of different crystal forms of 3-dehydroquinase from \fISalmonella typhi\fP and its implication for the enzyme activity. Acta Crystallographica, Section D. doi>
Shrive AK, Tharia HA, Strong P, Kishore U, Burns I, Rizkallah PJ, Reid KBM, Greenhough TJ. 2003. High-resolution Structural Insights into Ligand binding and Immune Cell Recognition by Human Lung Surfactant Protein D. Journal of Molecular Biology.
Hoole D, Lewis JW, Schuwerack PMM, Chakravarthy C, Shrive AK, Greenhough TJ, Cartwright JR. 2003. Inflammatory interactions in fish exposed to pollutants and parasites: a role for apoptosis and C reactive protein. Parasitology, S71-S85, vol. 126 Suppl. link> doi>
Hoole D, Lewis JW, Schuwerack PMM, Chakravarthy C, Shrive AK, Greenhough TJ, Cartwright JR. 2003. Inflammatory interactions in fish exposed to pollutants and parasites:a role for apoptosis and C-reactive protein. Parasitology.
Ramadan MAM, Shrive AK, Holden D, Myles DAA, Volanakis JE, DeLucas LJ, Greenhough TJ. 2002. The three-dimensional structure of calcium-depleted human C-reactive protein from perfectly twinned crystals. Acta Crystallogr D Biol Crystallogr, 992-1001, vol. 58(Pt 6 Pt 2). link> doi>
Tharia HA, Shrive AK, Mills JD, Arme C, Williams GT, Greenhough TJ. 2002. Complete cDNA sequence of SAP-like pentraxin from Limulus polyphemus: implications for pentraxin evolution. J Mol Biol, 583-597, vol. 316(3). link> doi>
Tharia HA, Shrive AK, Mills JD, Arme C, Williams GT, Greenhough TJ. 2002. Complete cDNA Sequence of SAP-like Pentraxin from \fILimulus polyphemus\fP:Implications for Pentraxin Evolution. Journal of Molecular Biology.
Ramadan MAM, Shrive AK, Holden D, Myles DAA, Volanakis JE, DeLucas LJ, Greenhough TJ. 2002. The three-dimensional structure of calcium-depleted human C-reactive protein from perfectly twinned crystals. Acta Crystallographica, Section D.
Agrawal A, Shrive AK, Greenhough TJ, Volanakis JE. 2001. Topology and structure of the C1q-binding site on C-reactive protein. J Immunol, 3998-4004, vol. 166(6). link> doi>
Shrive AK, Metcalfe AM, Cartwright JR, Greenhough TJ. 1999. C-reactive protein and SAP-like pentraxin are both present in Limulus polyphemus haemolymph: crystal structure of Limulus SAP. J Mol Biol, 997-1008, vol. 290(5). link> doi>
Gourley DG, Shrive AK, Polikarpov I, Krell T, Coggins JR, Hawkins AR, Isaacs NW, Sawyer L. 1999. The two types of 3-dehydroquinase have distinct structures but catalyze the same overall reaction. Nat Struct Biol, 521-525, vol. 6(6). link> doi>
Leech AP, Boetzel R, McDonald C, Shrive AK, Moore GR, Coggins JR, Sawyer L, Kleanthous C. 1998. Re-evaluating the role of His-143 in the mechanism of type I dehydroquinase from Escherichia coli using two-dimensional 1H,13C NMR. J Biol Chem, 9602-9607, vol. 273(16). link> doi>
Shrive AK, Holden D, Myles DA, Greenhough TJ. 1996. Structure solution of C-reactive proteins: molecular replacement with a twist. Acta Crystallogr D Biol Crystallogr, 1049-1057, vol. 52(Pt 6). link> doi>
Shrive AK, Cheetham GM, Holden D, Myles DA, Turnell WG, Volanakis JE, Pepys MB, Bloomer AC, Greenhough TJ. 1996. Three dimensional structure of human C-reactive protein. Nat Struct Biol, 346-354, vol. 3(4). link> doi>
SHRIVE AK and GREENHOUGH TJ. 1994. LAUE IMAGE-ANALYSIS .3. THE COLLECTION AND ANALYSIS OF DELIBERATELY STREAKED LAUE EXPOSURES. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 532-542, vol. 27. link> doi>
Hubbard SR, Greenall RJ, Shrive AK, Forsyth VT, Mahendrasingam A. 1994. Structure factor calculations for a side-by-side model of B-DNA. Int J Biol Macromol, 195-205, vol. 16(4). link> doi>
GREENHOUGH TJ and SHRIVE AK. 1994. LAUE IMAGE-ANALYSIS .2. VARIABLE RADIAL ELLIPTIC MASKING AND ITS APPLICATION IN STUDIES INVOLVING INHERENTLY STREAKED LAUE EXPOSURES. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 111-121, vol. 27. link> doi>
Campbell JW, Clifton IJ, Greenhough TJ, Hajdu J, Harrison SC, Liddington RC, Shrive AK. 1990. Calcium binding sites in tomato bushy stunt virus visualized by Laue crystallography. J Mol Biol, 627-632, vol. 214(3). link> doi>
SHRIVE AK, CLIFTON IJ, HAJDU J, GREENHOUGH TJ. 1990. LAUE FILM INTEGRATION AND DECONVOLUTION OF SPATIALLY OVERLAPPING REFLECTIONS. JOURNAL OF APPLIED CRYSTALLOGRAPHY, 169-174, vol. 23. link> doi>

Other

Williams R, Moran JA, Burns I, Greenhough TJ, Shrive AK. 2013. Structural Studies of C-reactive Protein. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. S317, vol. 69). link> doi>
da Silva R, Burns I, Greenhough TJ, Shrive AK. 2013. Structural Studies of Innate Immune Proteins. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. S322, vol. 69). link> doi>
Shrive A, Hood D, Clark H, Madsen J, Greenhough T. 2013. Structural basis of bacterial recognition by surfactant protein D. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. S358, vol. 69). link> doi>
Shrive AK, Shaw AJ, Ahmed A, Paterson JM, Smallcombe C, Reid KBM, Deadman ME, Hood DW, Holmskov U, Clark HW, Madsen J, Greenhough TJ. 2011. Structural basis of ligand and pathogen recognition by the collectins. Acta Crystallographica Section A: Foundations of Crystallography (p. C472, vol. A67). doi>
Greenhough TJ, Mold M, Moran J, Burns I, Paterson JM, Bailey SJ, Bailey J, Armstrong PB, Shrive AK. 2011. Structure, function and evolution of the serum pentraxins. Acta Crystallographica Section A: Foundations of Crystallography (p. C546, vol. A67). doi>
Shrive A, Martin C, Burns I, Paterson J, Martin J, Kishore U, Reid K, Greenhough T. 2005. Structural Basis of Ligand Recognition by the Collectins. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. C91, vol. 61). link> doi>
Greenhough T, Burns I, Paterson J, Armstrong P, Shrive A. 2005. Structure, Function and Evolution of the Serum Pentraxins. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. C230, vol. 61). link> doi>
Cartwright JR, MacCarthy E, Greenhough TJ, Shrive A, Wiegertjes GF, Hoole D. 2003. Pentraxin-like serum proteins from common carp \fI(Cyprinus carpio)\fP. Proceedings of the 11th International Conference of the European Association of Fish Pathologists.
Cartwright JR, MacCarthy E, Greenhough TJ, Shrive A, Wiegertjes GF, Hoole D. 2003. Pentraxin-like serum proteins from common carp \fI(Cyprinus carpio)\fP. Proceedings of the 11th International Conference of the European Association of Fish Pathologists.
Shrive AK, Tharia HA, Martin C, Burns I, Rizkallah PJ, Kishore U, Strong P, Reid KBM, Greenhough TJ. 2002. HIGH RESOLUTION NATIVE AND LIGAND-BOUND STRUCTURES OF AN ACTIVE TRIMERIC FRAGMENT OF HUMAN SP-D. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. C282, vol. 58). link> doi>
Greenhough TJ, Ramadan MAM, Shrive AK, Holden D, Myles DAA, DeLucas LJ, Volanakis JE. 2002. THE 3-DIMENSIONAL STRUCTURE OF Ca-DEPLETED HUMAN C-REACTIVE PROTEIN FROM PERFECTLY TWINNED CRYSTALS. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. C77, vol. 58). link> doi>
Cartwright JR, Greenhough TJ, Shrive AK, Hoole D. 2001. Isolation of C-reactive protein from the common carp \fI(Cyprinius carpio)\fP. Proceedings of the International Conference of the European Association of Fish Pathologists.
Porter DA, McNae I, Alexeev D, Sawyer L, Shrive AK, Polikarpov I, Coggins JR, Hawkins AR. 1999. STRUCTURAL COMPARISONS OF TYPE I 3-DEHYDRO-QUINASES. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. 287, vol. 55). link>
Greenhough TJ, Shrive AK, Ramadan MA, Metcalfe AM, Cartwright JR, Hopkins M. 1999. STRUCTURE, FUNCTION AND EVOLUTION OF SERUM PENTRAXINS FROM INVERTEBRATES TO HUMANS. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. 355, vol. 55). link>
Shrive AK, Cartwright JR, Metcalfe AM, Greenhough TJ. 1999. THE CRYSTAL STRUCTURE OF AN INVERTEBRATE PENTRAXIN-LECTIN WITH A NOVEL MOLECULAR ASSEMBLY. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. 197, vol. 55). link>
Ramadan MAM, Shrive AK, Greenhough TJ, Volanakis JE, DeLucas LJ. 1999. THE STRUCTURE OF CALCIUM-DEPLETED HUMAN C-REACTIVE PROTEIN FROM PERFECTLY TWINNED CRYSTALS. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. 350, vol. 55). link>
Shrive AK, Holden D, Metcalfe A, Myles DAA, Hopkins M, Glover ID, Hoole D, Bloomer AC, Greenhough TJ. 1996. STRUCTURE SOLUTION OF SOME C-REACTIVE PROTEINS. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. C240, vol. 52). link> doi>
Greenhough TJ, Cheetham GMT, Holden D, Myles DAA, Turnell WG, Volanakis JE, Pepys MB, Bloomer AC, Shrive AK. 1996. THREE DIMENSIONAL STRUCTURE OF HUMAN C-REACTIVE PROTEIN. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. C168, vol. 52). link> doi>
Hajdu J, Greenhough TJ, Clifton IJ, Campbell JW, Shrive AK, Harrison SC, Liddington RC. 1989. Laue crystallography: application to virus crystals. Basic Life Sci (pp. 331-339, vol. 51). link> doi>
Shrive A and Greenhough T. X-Ray diffraction data for structure associated with PDB ID: 6ZQY. doi>
Shrive A and Greenhough T. X-Ray diffraction data for structure associated with PDB ID: 6ZR4. doi>
Shrive A and Greenhough T. X-Ray diffraction data relating to protein structure PDB ID: 6RYG. Synchrotron station DLS I03, Diamond Light Source I03.doi>
Shrive A and Greenhough T. X-Ray diffraction data relating to protein structure PDB ID: 6RYM. Synchrotron station SRS 14.1, Daresbury SRS 14.1.doi>
Shrive A and Greenhough T. X-Ray diffraction data relating to protein structure PDB ID: 6RYM. Synchrotron station SRS 14.1, Daresbury SRS 14.1.doi>
Shrive A and Greenhough T. X-Ray diffraction data relating to protein structure PDB ID: 6RYN. Synchrotron station DLS I04, Diamond Light Source I04.doi> link>
Shrive A and Greenhough T. X-Ray diffraction data relating to protein structure PDB ID: 6RYN. Synchrotron station DLS I04, Diamond Light Source I04.doi> link>
Shrive A and Greenhough T. X-ray diffraction data for structure associated with PDB ID: 6ZQR. doi>
Shrive A and Greenhough T. X-ray diffraction data for structure associated with PDB ID: 6ZQX. doi>
Shrive A and Greenhough T. X-ray diffraction data for structure associated with PDB ID: 6ZR0. doi>
Shrive A and Greenhough T. X-ray diffraction data for structure associated with PDB ID: 6ZR3. doi>

Dr Annette K Shrive