Biography
In 2001 I obtained a BSc (Hons) in Biological Sciences with professional experience from Salford University. I commenced my PhD studies at the Robert Jones & Agnes Hunt (RJAH) Orthopaedic Hospital in Oswestry in 2005, and in 2009, was awarded my PhD in Biomedical Engineering from Keele University. Since then I have been employed as a Lecturer/Research Fellow/Research Associate of the Institute for Science & Technology in Medicine, Keele University based at the RJAH Hospital, Oswestry.
Research and scholarship
ISTM Research theme: Regenerative medicine
RESEARCH
Biology & pathology of musculoskeletal disorders and spinal cord injury
Cell-based therapies for the repair of musculoskeletal and spinal cord tissues
Bone marrow and umbilical cord derived stem cell biology (mesenchymal and hematopoietic)
Further information
OTHER INFORMATION
Collaborators:
• Prof Sally Roberts
Director of Spinal Research
RJAH Orthopaedic Hospital
• Prof James Richardson
Professor of Orthopaedic and Traumatic Surgery
RJAH Orthopaedic Hospital
• Dr Eustace Johnson
Professor of Stem Cells and Regenerative Biology
Senior Faculty Postgraduate Research Tutor Faculty Research Co-ordinator Biological Sciences
Faculty of Medicine Dentistry and Life Sciences
University of Chester
• Dr Gareth Griffiths
Director of Science
Imagenbiotech
University of Manchester
• Mr Wagih S El Masry
Consultant Surgeon in Spinal Injuries
Former President of International Spinal Cord Society (ISCOS)
RJAH Orthopaedic Hospital
• Mr Aheed Osman
Consultant Surgeon in Spinal Injuries
Midland Centre for Spinal Injuries
RJAH Orthopaedic Hospital
• Mr J Roy Chowdhury
Consultant in Spinal Injuries & Rehabilitation Medicine
Midlands Centre for Spinal Injuries
RJAH Orthopaedic Hospital
PUBLICATIONS
- Wright KT, El Masri W, Osman A, Chowdhury J, Johnson WEB. Bone marrow for the treatment of spinal cord injury: mechanisms and clinical application. (2011) Stem Cells, Volume 29, 169-178.
- Wright KT, Seabright RJ, Logan A, Lilly AJ, Khanim F, Bunce CM, Johnson WEB. Extracellular Nm23H1 stimulates neurite outgrowth from dorsal root ganglia neurons in vitro independently of nerve growth factor supplementation or its nucleoside diphosphate kinase activity. (2010). Biochemical and Biophysical Research Communications, Volume 398, 79-85.
- Walter MN, Wright KT, Fuller HR, MacNeil S, Johnson WEB. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: an in vitro study of fibroblast and keratinocyte scratch assays. (2010) Experimental Cell Research, Volume 316, Number 7; 1271-1281.
- Wright KT, Griffiths G, Johnson WEB. A comparison of industrial high content analysis versus research-based manual analysis to assay the effects of mesenchymal stem cell conditioned medium on neurite outgrowth in vitro. (2010) Journal of Biomolecular Screening, Volume 15, Issue 5; 576-582.
- Bajada S, Marshall MJ, Wright KT, Richardson JB, Johnson WEB. Decreased osteogenesis, increased cell senescence and elevated dickkopf-1 secretion in human fracture non union stromal cells. (2009) Bone. Volume 45, Issue 4; 726-735.
- Wright KT, Griffiths G, Johnson WEB. A comparison of industrial high content analyses versus research lab-based methods to assay the effects of mesenchymal stem cell conditioned medium on neurite outgrowth in vitro. Abstract (2009) Neurorehabilitation and Neural Repair, Volume 23, Number 9, Page 984.
- Chamberlain G, Wright KT, Rot A, Ashton BA, Middleton J. Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. (2008) PLoS ONE, Volume 3; 1-6.
- Wright KT, El Masri W, Osman A, Roberts S, Trivedi J, Ashton BA, Johnson WEB. The cell culture expansion of bone marrow stromal cells from humans with spinal cord injury: implications for future cell transplantation therapy. (2008) Spinal Cord, Volume 46; 811-817.
- Wright KT, El Masri W, Osman A, Roberts S, Ashton BA, Johnson WEB. Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A. (2007) Biochemical and Biophysical Research Communications, Volume 354; 559-566.
- Wright KT, Khanim F, Bunce CM, Johnson WEB. The nucleoside diphosphatase kinase, nm23H1, is expressed by human bone marrow stromal cells and stimulates and directs neurite outgrowth in vitro. Abstract (2007) Neurorehabilitation and Neural Repair, Volume 21, Number 6, Page 62.
- Johnson WEB, Sivan S, Wright KT, Eisenstein SM, Maroudas A, Roberts S. Human intevertebral disc cells promote nerve growth over substrata of human intevertebral disc aggrecan. (2006) Spine, Volume 31, Number 11; 1106-1114.
- Wright KT, El Masri W, Osman A, Roberts S, Ashton BA, Johnson WEB. Marrow stromal cells from spinal cord injured patients promote nerve growth and reduce the inhibitory effects of chondroitin sulphated proteoglycans in vitro. Abstract (2006) Journal of Spinal Cord Medicine, Volume 29, Number 3, Page 270.
Selected Publications
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Characterization of regional meniscal cell and chondrocyte phenotypes and chondrogenic differentiation with histological analysis in osteoarthritic donor-matched tissues. Sci Rep, vol. 10(1), 21658. link> doi> full text>2020.
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Routinely Measured Hematological Markers Can Help to Predict American Spinal Injury Association Impairment Scale Scores after Spinal Cord Injury. J Neurotrauma. link> doi> full text>2020.
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A Preliminary Cohort Study Assessing Routine Blood Analyte Levels and Neurological Outcome after Spinal Cord Injury. J Neurotrauma, vol. 37(3), 466-480. link> doi> full text>2020.
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Combined Autologous Chondrocyte and Bone Marrow Mesenchymal Stromal Cell Implantation in the Knee: An 8-year Follow Up of Two First-In-Man Cases. Cell Transplant, vol. 28(7), 924-931. link> doi> full text>2019.
- 2019.
Full Publications List show
Journal Articles
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Characterization of regional meniscal cell and chondrocyte phenotypes and chondrogenic differentiation with histological analysis in osteoarthritic donor-matched tissues. Sci Rep, vol. 10(1), 21658. link> doi> full text>2020.
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Routinely Measured Hematological Markers Can Help to Predict American Spinal Injury Association Impairment Scale Scores after Spinal Cord Injury. J Neurotrauma. link> doi> full text>2020.
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A Preliminary Cohort Study Assessing Routine Blood Analyte Levels and Neurological Outcome after Spinal Cord Injury. J Neurotrauma, vol. 37(3), 466-480. link> doi> full text>2020.
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Combined Autologous Chondrocyte and Bone Marrow Mesenchymal Stromal Cell Implantation in the Knee: An 8-year Follow Up of Two First-In-Man Cases. Cell Transplant, vol. 28(7), 924-931. link> doi> full text>2019.
- 2018.
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Impact of human platelet lysate on the expansion and chondrogenic capacity of cultured human chondrocytes for cartilage cell therapy. Eur Cell Mater, vol. 35, 255-267. link> doi> full text>2018.
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Human Articular Chondrocytes Retain Their Phenotype in Sustained Hypoxia While Normoxia Promotes Their Immunomodulatory Potential. Cartilage. doi> link> full text>2018.
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Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation. Arthritis Res Ther, vol. 20(1), 87. link> doi> full text>2018.
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Autologous chondrocyte implantation-derived synovial fluids display distinct responder and non-responder proteomic profiles. Arthritis Res Ther, vol. 19(1), 150. link> doi> full text>2017.
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Efficacy and safety of autologous cell therapies for knee cartilage defects (autologous stem cells, chondrocytes or the two): randomized controlled trial design. Regen Med, vol. 12(5), 493-501. link> doi> full text>2017.
- 2017.
- 2017.
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The Absence of Detectable ADAMTS-4 (Aggrecanase-1) Activity in Synovial Fluid Is a Predictive Indicator of Autologous Chondrocyte Implantation Success. Am J Sports Med, vol. 45(8), 1806-1814. link> doi> full text>2017.
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The developing landscape of diagnostic and prognostic biomarkers for spinal cord injury in cerebrospinal fluid and blood. Spinal Cord, vol. 55(2), 114-125. link> doi> full text>2017.
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Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat. Stem cells international, vol. 2016, Article 6969726. doi> link> full text>
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Characterisation of synovial fluid and infrapatellar fat pad derived mesenchymal stromal cells: The influence of tissue source and inflammatory stimulus. Sci Rep, vol. 6, 24295. link> doi> full text>2016.
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Temporal Analyses of the Response of Intervertebral Disc Cells and Mesenchymal Stem Cells to Nutrient Deprivation. Stem Cells Int, vol. 2016, 5415901. link> doi> full text>2016.
- 2015.
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ADAMTS-4 activity in synovial fluid as a biomarker of inflammation and effusion. Osteoarthritis Cartilage, vol. 23(9), 1622-1626. link> doi> full text>2015.
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High content and high throughput screening to assess the angiogenic and neurogenic actions of mesenchymal stem cells in vitro. Exp Cell Res, vol. 333(1), 93-104. link> doi> full text>2015.
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Human mesenchymal stem cells stimulate EaHy926 endothelial cell migration: combined proteomic and in vitro analysis of the influence of donor-donor variability. J Stem Cells Regen Med, vol. 11(1), 18-24. link> full text>2015.
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Spinal motor neurite outgrowth over glial scar inhibitors is enhanced by coculture with bone marrow stromal cells. Spine J, vol. 14(8), 1722-1733. link> doi> full text>2014.
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Characterization of the cells in repair tissue following autologous chondrocyte implantation in mankind: a novel report of two cases. Regen Med, vol. 8(6), 699-709. link> doi> full text>2013.
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Isolation and characterisation of mesenchymal stem cells from different regions of the human umbilical cord. Biomed Res Int, vol. 2013, 916136. link> doi> full text>2013.
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Transplantation of Mesenchymal Stem Cells Promotes an Alternative Pathway of Macrophage Activation and Functional Recovery after Spinal Cord Injury. JOURNAL OF NEUROTRAUMA, vol. 29(8), 1614-1625. link> doi> full text>2012.
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Concise review: Bone marrow for the treatment of spinal cord injury: mechanisms and clinical applications. Stem Cells, vol. 29(2), 169-178. link> doi> full text>2011.
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Bone Marrow for the Treatment of Spinal Cord Injury: Mechanisms and Clinical Application. Stem Cells. doi> full text>2010.
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Extracellular Nm23H1 stimulates neurite outgrowth from dorsal root ganglia neurons in vitro independently of nerve growth factor supplementation or its nucleoside diphosphate kinase activity. Biochem Biophys Res Commun, vol. 398(1), 79-85. link> doi> full text>2010.
-
A comparison of high-content screening versus manual analysis to assay the effects of mesenchymal stem cell-conditioned medium on neurite outgrowth in vitro. J Biomol Screen, vol. 15(5), 576-582. link> doi> full text>2010.
- 2010.
-
Decreased osteogenesis, increased cell senescence and elevated Dickkopf-1 secretion in human fracture non union stromal cells. Bone, vol. 45(4), 726-735. link> doi> full text>2009.
-
The cell culture expansion of bone marrow stromal cells from humans with spinal cord injury: implications for future cell transplantation therapy. Spinal Cord, vol. 46(12), 811-817. link> doi> full text>2008.
-
Murine mesenchymal stem cells exhibit a restricted repertoire of functional chemokine receptors: comparison with human. PLoS One, vol. 3(8), e2934. link> doi> full text>2008.
-
Bone marrow stromal cells stimulate neurite outgrowth over neural proteoglycans (CSPG), myelin associated glycoprotein and Nogo-A. Biochemical and Biophysical Research Communications, vol. 354(2), 559-566. doi>2007.
-
Human intervertebral disc cells promote nerve growth over substrata of human intervertebral disc aggrecan. Spine, vol. 31, 1187-1193. doi>2006.
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Identification of Candidate Synovial Fluid Biomarkers for the Prediction of Patient Outcome following Microfracture or Osteotomy. American Journal of Sports Medicine. full text>
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Proteomic investigation of systemic response to spinal cord injury in rodent models. Spinal Cord.
Other
- 2019.
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Identification of synovial fluid proteins that are associated with early osteoarthritis treatment failure: the search for novel markers leads us back to matrix metalloproteinases (MMPs). INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 99, p. A2). link>2018.
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ACUTE PHASE RESPONSE SIGNALLING IS ALTERED FOLLOWING CARTILAGE HARVEST IN NON-RESPONDERS TO AUTOLOGOUS CHONDROCYTE IMPLANTATION. OSTEOARTHRITIS AND CARTILAGE (vol. 26, p. S167). link> doi> full text>2018.
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ASSESSMENT OF CANDIDATE PREDICTIVE PROTEIN BIOMARKERS FOR MICROFRACTURE AND OSTEOTOMY. OSTEOARTHRITIS AND CARTILAGE (vol. 26, pp. S191-S192). link> doi> full text>2018.
- 2018.
- 2018.
- 2018.
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ASSESSING THE CHARACTERISTICS OF HUMAN MESENCHYMAL STROMAL CELLS DERIVED FROM BONE MARROW AND UMBILICAL CORD FOLLOWING EXPANSION IN THE QUANTUM (R) HOLLOW-FIBRE BIOREACTOR SYSTEM. OSTEOARTHRITIS AND CARTILAGE (vol. 26, pp. S146-S147). link> full text>2018.
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Similar phenotypes of cells in the repair tissues following microfracture or autologous chondrocyte implantation. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 98, p. A23). link>2017.
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CHARACTERISTICS OF HUMAN BONE MARROW MSCS ARE SENSITIVE TO MONONUCLEAR CELL SEPARATION TECHNIQUE AND OXYGEN TENSION. CYTOTHERAPY (vol. 19, p. S119). link>2017.
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FACILITATING THE OPERATIONAL READINESS OF THE NHS FOR THE IN-HOUSE MANUFACTURE AND DELIVERY OF AUTOLOGOUS CELL THERAPY. CYTOTHERAPY (vol. 19, p. S89). link>2017.
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POOR CLINICAL RESPONSE TO AUTOLOGOUS CHONDROCYTE IMPLANTATION IS ASSOCIATED WITH A UNIQUE SYNOVIAL FLUID PROTEOME PROFILE. OSTEOARTHRITIS AND CARTILAGE (vol. 25, p. S92). link> doi> full text>2017.
- 2016.
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Cartilage harvest elevates sCD14 and MMP-3 in the synovial fluid of the autologous chondrocyte implantation (ACI) treated joint. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 97, p. A24). link>2016.
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The response of intervertebral disc cells and mesenchymal stromal cells to altered levels of glucose and serum in vitro. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 95, p. A34). link>2014.
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A Computational Approach to Quantifying Axon Regeneration in the Presence of Mesenchymal Stem Cells (MSCs). 2013 6TH INTERNATIONAL IEEE/EMBS CONFERENCE ON NEURAL ENGINEERING (NER) (pp. 1541-1544). link>2013.
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Influence of small proteoglycans on nerve growth in the intervertebral disc. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 92, p. A12). link>2011.
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The small leucine-rich proteoglycan, opticin, is inhibitory to neurite extension and endothelial migration and is present in the neonatal intervertebral disc and vertebral growth plate. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 88, p. A65). link>2007.
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Can we identify altered biological pathways and novel candidate biomarkers in individuals who do respond clinically following Autologous Chondrocyte Implantation?. European Cells and Materials.
More
PhD Students
John Garcia (Lead Supervisor 2013-2016)
Shiraz Ziya (Lead Supervisor 2014-present)
Soukaina Bahsoun (Co-supervisor 2016-present)
Jessica Sykes (Co-supervisor 2016-present)
Tim Hopkins (Co-supervisor 2016-present)
Amy Byrne (Co-supervisor 2016-present)
Equipment
BD FACS canto II flow cytometer (Arthritis Research UK Equipment Grant – PI)
Cell IQ II Live Cell Imaging Platform (Institute of Orthopaedics Equipment Grant – Co-I)
Funding
Arthritis Research UK Project Grant (PI)
Arthritis Research UK Tissue Engineering Centre (Co-I)
Medical Research Council Experimental Medicine Grant (Co-I)
Institute of Orthopaedics and Midlands Centre for Spinal Injuries (PI)
Team Members
Dr Charlotte Hulme (Post-Doctoral Research Associate FT)
Dr Sharon Owen (Post-Doctoral Research Associate PT)


