ISTM

Dr Josep Sulé-Suso qualified in Medicine and Surgery at the University of Barcelona. He trained in oncology at the University Hospital of North Staffordshire. He later furthered his research at the Instituto Nazionale dei Tumori in Milan, Italy. He presently combines his clinical duties at the Cancer Centre, University Hospital of North Staffordshire with his research at ISTM.

ISTM Research theme: 1. Clinical & Diagnostic Science

Research Experience

Cancer immunotherapy, cancer cell biology, translational research, clinical trials, vibrational spectroscopy, synchrotron based FTIR spectroscopy.

Research Funding

Journals

1. Smith D., Špan?l P., and Sulé-Suso J. Advantages of Breath Testing for Early Diagnosis of Lung Cancer. Expert Review of Molecular Diagnostics. Editorial 10: 255-257; 2010.
2. Pijanka J. K., Stone N., Cinque G., Yang Y., Kohler A., Wehbe K., Frogley M., Parkes G., Parkes J., Dumas P., Sandt C., van Pittius D. G., Douce G., Sockalingum G. D., and Sulé-Suso J. FTIR microspectroscopy of stained cells and tissues. Application in cancer diagnosis. Spectroscopy-Biomedical applications 24: 73-78; 2010.
3. Pijanka J., Sockalingum G. D., Kohler A., Yang Y., Draux F., Dumas P., Sandt C., Parkes G., van Pittius D. G, Douce G., Untereiner V. and Sulé-Suso J. Synchrotron based FTIR spectra of stained single cells. Towards a clinical application in pathology. Laboratory Investigation 90: 797-807; 2010.
4. Sulé-Suso J., Pysanenko A., Špan?l P., and Smith D. Generation of acetaldehyde and carbon dioxide by malignant and non-malignant lung cell lines in vitro as measured by SIFT-MS. Analyst 134: 2419-2425; 2009
5. Draux F., Jeannesson P., Gobinet C, Sulé-Suso J., Pijanka J., Sandt C., Dumas P., Manfait M., and Sockalingum G. D. IR spectroscopy evidences effect of non-cytotoxic (cytostatic) doses of antitumor drug on cancer cells Analytical and Bioanalytical Chemistry 395: 2293-2301; 2009.
6. Pijanka J. K., Yang Y., Bagnaninchi P. O., Debelle L., Sockalingum G. D., and Sulé-Suso J. Elastin peptides increase lung cancer cell growth in 3D models. Proceedings of SPIE, Volume 7179: 717907/01-717907/11; 2009.
7. Egot-Lemaire S., Pijanka J., Sulé-Suso J., and Semenov S. Dielectric spectroscopy of normal and malignant human lung cells in the ultra-high frequencies. Physics in Medicine and Biology 54 (8): 2341-2357. 2009.
8. Pijanka J., Kohler A., Yang Y., Dumas P., Chio-Srichan S., Manfait M., Sockalingum G. D., and Sulé-Suso J. FTIR Microscopy. Spectroscopic Signatures of Single, Isolated Cancer Cell Nuclei using Synchrotron Infrared microscopy. Analyst 134: 1176-1181; 2009.
9. Draux F., Jeannesson P., Beljebbar A., Tfayli A., Fourre N., Manfait M., Sulé-Suso J., and Sockalingum G. D. Raman Spectral Imaging of Single Living Cancer Cells. Analyst 134 (3): 542-8; 2009.
10. Yang Y., Sulé-Suso J., and Sockalingum G. D.Study of melanoma invasion by FTIR spectroscopy. Proceedings of SPIE, Vol. 68591U: 1-7; 2008.
11. Kohler A., Sulé-Suso J., Sockalingum G D, et al. Estimating and correcting Mie scattering in synchrotron-based microscopic FTIR spectra by extended multiplicative signal correction (EMSC) Applied Spectroscopy 62: 259-266; 2008.
12. Egot-Lemaire S., Bagnaninchi P.-O., Pijanka J., Sulé-Suso J., and Semenov S., Microwave Dielectric Spectroscopy of Low-volume Fraction Human Cancer Cells Embedded in Collagen Gels. Proceedings of “BIODEVICES 2008”, International Conference on Biomedical Electronics and Devices, Vol 2: 156-161; Funchal, Madeira, Portugal.
13. Dumas P., Sockalingum G. D., and Sulé-Suso J. Adding synchrotron radiation to infrared microspectroscopy: what’s new in biomedical applications? Trends in Biotechnology 25: 40-44; 2007. Invited review.
14. Yang Y., Hoban P., Sulé-Suso J., Holley S., El Haj A. J., Bahrami F., and Wang R. K. Study of cell invasion by optical techniques. Proceedings of SPIE, Volume 6084: 60841A1-60841A8; 2006.
15. Yang Y., Sulé-Suso J., Sockalingum G. D., Kegelaer G., Manfait M., El Haj A. J. Study of tumour cell invasion by microscopic Fourier Transform Infrared Spectroscopy. Biopolymers 78: 311-317; 2005.
16. Sulé-Suso J., Skingsley D., Sockalingum G. D., Kohler A., Kegelaer G., Manfait M., and El Haj A. J. FTIR microspectroscopy as a tool to assess lung cancer cells response to chemotherapy. Vibrational Spectroscopy 38: 179-184; 2005.
17. Yang Y., Sulé-Suso J., El Haj A., Hoban P. R., and Wang R. Monitoring of lung tumour cell growth in artificial membranes. Biosensors and Bioelectronics 20: 442-447; 2004
18. Sulé-Suso J., Forster A., Zholobenko V., Stone N., and El Haj A. Effects of CaCl2 and MgCl2 on Fourier Transform Infrared spectra of lung cancer cells. Applied Spectroscopy 58: 61-67; 2004.
19. Yang Y., Sulé-Suso J., El Haj A., Hoban P. R., and Wang R. Use of OCT to study tumour cell growth in 3D membranes. Use of OCT to study tumour cell growth in 3D membranes Proceedings of SPIE, Vol 5486: 154-160; 2003.
20. Smith D., Wang T., Sulé-Suso J., Špan?l P., and El Haj A. Quantification of acetaldehyde released by lung cancer cells in vitro using selected ion flow tube mass spectrometry. Rapid Communications in Mass Spectrometry 17: 845-850; 2003.
21. Arienti F., Belli F., Napolitano F., Sulé-Suso J., Mazzocchi A., Gallino G. F., Cattelan A., Sanantonio C., Rivoltini L., Melani C., Colombo M. P., Cascinelli N., Maio M., Parmiani G: Vaccination of melanoma patients with IL-4 gene-transduced allogeneic melanoma cells. Human Gene Therapy, 10: 2907-2916 (1999).
22. Belli F., Arienti F., Sulé-Suso J., Clemente C., Mascheroni L., Cattelan A., Sanantonio C., Gallino G. F., Melani C., Rao S., Colombo M. P., Maio M., Cascinelli N., and Parmiani G. Active immunisation of metastatic melanoma patients with interleukin-2-transduced allogeneic melanoma cells: evaluation of efficacy and tolerability. Cancer Immunology Immunotherapy, 44: 197-203 (1997).
23. Arienti F., Sulé-Suso J., Belli F., Mascheroni L., Rivoltini L., Melani C., Maio M., Cascinelli N., Colombo M. P., and Parmiani G. Limited antitumour T cell response in melanoma patients vaccinated with interleukin-2 gene transduced allogeneic melanoma cells. Human Gene Therapy, 7: 1955-1963 (1996).
24. Parmiani G., Arienti F., Sulé-Suso J., Melani C., Colombo M. P., Ramakrishna V., Belli F., Mascheroni L., Rivoltini L., and Cascinelli N. Cytokine-based gene therapy of human tumours. An overview. Folia Biologica 42: 305-309 (1996).
25. Melani C., Sulé-Suso J., Arienti F., Maccalli C., Passerini F., Colombo M. P., and Parmiani G. A human melanoma cell line transduced with an interleukin-4 gene by a retroviral vector releases biologically active IL-4 and maintains the original tumour antigenic phenotype. Human Gene Therapy, 6: 1427-1436 (1995).
26. Sulé-Suso J., Arienti F., Melani C., Colombo M. P., and Parmiani G. A B7-1 transduced human melanoma cell line stimulates proliferation and cytotoxicity of autologous and/or allogeneic PBL. European Journal of Immunology, 25: 2737-2742 (1995).
27. Arienti F., Sulé-Suso J., Melani C., Maccalli C., Belli F., Illeni M. T., Anichini A., Cascinelli N., Colombo M. P., and Parmiani G. IL-2 gene transduced human melanoma cells efficiently stimulate MHC-unrestricted and MHC-restricted autologous lymphocytes. Human Gene Therapy, 5: 1139-1150 (1994).
28. Cascinelli N., Foà R., Parmiani G., Arienti F., Belli F., Bernengo M. G., Clemente C., Colombo M. P., Guarini A., Illeni M. T., Mascheroni L., Melani C., Prada A., and Sulé-Suso J. Clinical Protocol: Active immunisation of metastatic melanoma patients with interleukin-4 transfected, allogeneic melanoma cells. A phase I-II study. Human Gene Therapy, 5: 1059-1064 (1994).
29. Melani C., Chiodoni C., Arienti F., Maccalli C., Sulé-Suso J., Anichini A., Colombo M. P., and Parmiani G. Cytokine gene transduction in tumour cells: IL-2 or IL-4 gene transfer in human melanoma cells. Natural Immunity, 13: 76-84 (1994).
30. Sulé-Suso J., and Brunt A. M. Oat cell carcinoma of the prostate. British Journal of Radiology, 65: 726-728 (1992).
31. Sulé-Suso J., Brunt A. M., Lindup R., and Scoble J. E. Hyperfractionated accelerated radiotherapy for carcinoma of the oesophagus. Clinical Oncology, 3: 209-213 (1991).

32. Draux F., Gobinet C., Sulé-Suso J., Trussardi A., Manfait M., Jeannesson P., and Sockalingum G. D. Raman spectral imaging of single cancer cells: probing the impact of sample fixation methods. Analytical Bioanalytical Chemistry 397:2727-2737; 2010.

33. Sulé-Suso J. and Cinque G. Fourier Transform Infrared Spectroscopy in cancer diagnosis. Do we need a synchrotron? Microscopy and Analysis 140: 25-28; 2010.

34. Pijanka J. K., Kumar D., Dale T., YousefI., Parkes G., Untereiner V., Yang Y., Dumas P., Collins D., Manfait M., Sockalingum G. D., Forsyth N. R., and Sulé-Suso J. Vibrational spectroscopy differentiates between Multipotent and pluripotent stem cells. Analyst 135: 3126-3132; 2010.

35. Sule-Suso J. Towards IR fingerprinting of cancer ex-vivo: pilot studies on stained cells with micro IR spectroscopy. Diamond Light Source Annual Report, pp 72-73, 2010.

36. Draux F., Gobinet C., Sulé-Suso J., Manfait M., Jeannesson P., and Sockalingum G. D. Raman imaging of single living cells: probing effects of non cytotoxic doses of an anti-cancer drug. Analyst (in press)

37. Aydin H. M., Hu B., Sulé-Suso J., El Haj J., and Yang Y. Study of Tissue Engineered Bone Nodules by Fourier Transform Infrared Spectroscopy. Analyst (in press).

Book chapters

• Sulé-Suso J., Arienti F., Belli F., Melani C., Mascheroni L., Colombo M. P., Cascinelli N., and Parmiani G. Vaccination of melanoma patients with interleukin-2 transduced, allogeneic melanoma cells: an overview. In “The Internet Book of Gene Therapy. Cancer Therapeutics”. Sobol R. E. and Scanlon K. J., Eds. Appleton and Lange, Stamford, Connecticut, U. S. A. (1996).

• Sulé-Suso J. Synchrotron based FTIR spectroscopy in lung cancer. Is there a niche? In: Biomedical Applications of Synchrotron Infrared Microspectroscopy. In: Biomedical Applications of Synchrotron Infrared Microspectroscopy. RSC Publishing,London, U. K., Editor: Moss D. 2011.

• Fisher S. E., Harris A. T, Khanna N. and Sulé-Suso J. Vibrational Spectroscopy. What does the clinician need? In: Biomedical Applications of Synchrotron Infrared microspectroscopy. In: Biomedical Applications of Synchrotron Infrared Microspectroscopy. RSC Publishing,London, U. K., Editor: Moss D. 2011.

Conference papers over the last 5 years

1. Pijanka J. K., Sockalingum G. D., Kohler A., Yang Y., Draux F., Dumas P., Cinque G., Stone N., Untereiner V., and Sulé-Suso J. FTIR spectra of stained cells: towards a clinical application in pathology. Spec2010,Manchester, U. K. June-July, 2010.

2. Pijanka J. K., Kumar D., Sockalingum G. D., Yousef I., Untereiner V., Dumas P., Yang Y., Collins D., Forsyth N., .and Sulé-Suso J. Characterization of human (embryonic and mesenchymal) stem cells by Synchrotron Fourier Transform Infrared Microspectroscopy. Spec2010,Manchester, U. K. June-July, 2010.

3. Aydin H. M., Sulé-Suso J., Hu B., El Haj A., and Yang Y. FTIR study of tissue engineered bone nodules. Spec2010,Manchester, U. K. June-July, 2010.

4. Draux. F., Gobinet C., Tfayli A., Sulé-Suso J., Manfait M., Jeannesson P., and Sockalingum G. D. Raman imaging evidences effects of non-cytotoxic doses of anti-cancer drug on living cells. Spec2010,Manchester, U. K. June-July, 2010.

5. Draux F., Jeannesson P., Beljebbar A., Sulé-Suso J., Manfait M., and Sockalingum G. D. Probing single living lung cancer cells using micro-ramanspectroscopy. 16ème Edition des Journées du Groupe Français de Spectroscopie Vibrationelle. Metz, France, June 2009.
6. Lam K. P., Collins D., Sulé-Suso J., and Bhana R. Active CT Image visualization environment (ACTIVE) – An investigative study of automated scale selection. 13th International Conference on Information Visualisation, Barcelona, July 2009.
7. Sulé-Suso J., Pijanka J. K., Bagnaninchi P. O., and Yang Y. Effect of elastin on lung cancer cell growth using optical coherence tomography. SPIE 2009, San José, USA, January 2009.
8. Sulé-Suso J., Pijanka J., Kohler A., Yang Y., Dumas P., Chio-Srichan S., Manfait M., and Sockalingum G. D. FTIR Microscopy Probing of Single, Isolated Cancer Cell Nuclei with a Third Generation Synchrotron Source. Spec2008, São José dos Campos, Brasil, October 2008.
9. Yang Y., Sulé-Suso J., Sockalingum G. D. Study of melanoma invasion by FTIR spectroscopy. Spec2008, São José dos Campos, Brasil, October 2008.
10. Draux F., Jeannesson P., Sulé-Suso J., Manfait M., and Sockalingum G. D. Probing Single Live Lung Cancer Cells Using Micro-Raman Spectroscopy. Spec2008, São José dos Campos, Brasil, October 2008.
11. Draux F., Jeannesson P., Sulé-Suso J., Manfait M., and Sockalingum G. D. Probing Single Live Lung Cancer Cells Using Micro-Raman Spectroscopy. Proc 98th Amer Assoc Cancer Res, USA, Abstract No: 5135. San Diego, USA. April 2008.
12. Draux F., Jeannesson P., Beljebbar A., Tfayli A., Fourré N., Manfait M., Sulé-Suso J., and Sockalingum G. D. Raman Microspectroscopy of Single Alive Lung Cancer Cells. A Feasibility Study. Workshop: Chimiométrie des Spectroscopies et Imageries Moléculaires. Anglet, France. October 2007.
13. Draux F., Beljebbar A., Tfayli A., Fourré N., Jeannesson P., Manfait M., Sulé-Suso J., and Sockalingum G. D. Raman Microspectroscopy of Single Alive Lung Cancer Cells. A Feasibility Study. 12th European Conference on the Spectroscopy of Biological Molecules. Bobigny, France. September 2007.
14. Sulé-Suso J., Sockalingum G. D., Kohler A., Bahrami F., Cotte M., Pijanka J. K., Yang Y., Manfait M., and Dumas P. Synchrotron based FTIR spectroscopy of single cells. Applications in lung cancer diagnosis and management. 12th World Conference on Lung Cancer. Seoul, South Korea. September 2007.
15. Kohler A., Sulé-Suso J., Sockalingum G. D., Tobin M., Bahrami F., Yang Y., Pijanka J., Dumas P., Cotte M., van Pittius D. G., Parkes G., and Martens H. Estimating and correcting Mie scattering in synchrotron based microscopic FTIR spectra by extended multiplicative signal correction (EMSC). 1st International Workshop on spectra diagnosis (ISD-1). Boston, USA. June 2007.
16. Manfait M., Draux F., Sulé-Suso J., Fourre N., Tfayli A., Jeannesson P., and Sockalingum G. D. Raman Microspectroscopy of Single In vitro Growing Lung Cancer Cells. 1st Asian Spectroscopy Conference and Asian Biospectroscopy Conference. Bangalore, India. January-February 2007.
17. Yang Y., Sulé-Suso J., Sockalingum G. D., Wang R. K., and El Haj A. J. Study of cell invasion by optical techniques. 1st Asian Spectroscopy Conference and Asian Biospectroscopy Conference. Bangalore, India. January-February 2007.
18. Sulé-Suso J., Sockalingum G. D., Srichan S., Kohler A., Tobin M., Bahrami F., Cotte M., Pijanka J., Yang Y., Dumas P. Synchrotron based FTIR spectroscopy of single cells. An insight into advantages and difficulties. 1st Asian Spectroscopy Conference and Asian Biospectroscopy Conference. Bangalore, India. January-February 2007.
19. Draux F., Tfayli A., Fourre N., Jeannesson P., Manfait M., Sulé-Suso J., Sockalingum G. D. Micro-Raman Spectroscopy of in vitro growing single alive lung cancer cells. Spec2006: Shedding light on disease: optical diagnosis for the new millennium. Heidelberg, Germany. May 2006.
20. Kohler A, Hanafi M., Bertrand D., Sulé-Suso J., Sockalingum G. D., and Qannari E. M. Understanding co-variant patterns in biological data by the use of multiblock methods. Spec2006: Shedding light on disease: optical diagnosis for the new millennium. Heidelberg, Germany. May 2006.
21. Manfait M., Sockalingum G. D., Skingsley D., Kohler A., Jeannesson P., El Haj A. J., and Sulé-Suso J. Vibrational Microspectroscopy as a New Tool to Assess Response of In Vitro Growing Lung Cancer Cells to Gemcitabine. Proc 97th Amer Assoc Cancer Res, Abstract No: 1390; 1-5 April, Washington, USA, 2006.
22. Ying Y., Wang. R., Sulé-Suso J., and El Haj A. Study of cell invasion by optical techniques. Photonics West, San José, California, USA. January, 2006.

Education

2003    PhD in Medicine, Institute for Science and Technology in Medicine, Keele University.
Thesis: Autologous cell approaches to diagnosis and therapy in oncology.  Date of award: 04-06-2003
1987    Licentiate in Medicine and Surgery, Barcelona, Catalonia, Spain.

Employment experience

10/06 – Present Post      Clinical Senior Lecturer, Keele University.
10/04 – 09/06                Clinical Lecturer, Keele University.
11/03 – Present Post      Associate Specialist in Clinical Oncology. University Hospital of North Staffordshire.

06/95 – 11/03                Staff Grade in Clinical Oncology. University Hospital of North Staffordshire.
02/93 – 05/95                Research Fellow. Department of Experimental Oncology “D”, National Cancer Institute, Milan (Italy).
11/88 – 12/92                Senior House Officer. University Hospital of North Staffordshire.

Professional activities

- Chairman of the Working Group for the Infrared Beamline at DIAMOND synchrotron, Oxfordshire, 2007.
- Member of the discussion panel: “Microscopy and Imaging in the Synchrotron Light”, organised by the French Embassy in London, at the Royal Society of Medicine, January, 2006

International advisory board member

1. Spec2010, June 2010, Manchester UK, member of the local organising committee.
2. 3rd Diagnostic Applications with Synchrotron Infrared Micro-Spectroscopy Meeting, DASIM-2007. Soleil synchrotron, Paris, France.
3. 4th International Symposium “Shedding Light on Disease: Optical Diagnosis for the New Millennium”. Heidelberg, Germany. June 2006

Examiner

1. External examiner for the PhD thesis: “Infrared microspectroscopy of cancerous and non-cancerous cells”,DublinInstitute ofTechnology,Ireland, December 2010.

2. External examiner for the PhD thesis: “Infrared microspectroscopy of cancerous and non-cancerous cells“, UniversityofLancaster, November 2010.

3. Member of the jury examining the PhD Thesis “Synchrotron Based Fourier Transform Infrared Microspectroscopy and Fluorescence Microscopy: Application on Photodynamic Treated Cancer Cells” University of Paris-7 (Denis Diderot), France, December 2009.
4. Member of the jury examining the PhD Thesis “Analysis of the cancer cell and its interaction with an anticancer agent”,  University of Reims, France, November 2009.
5. External examiner for the PhD thesis: “Evaluation of metastatic status of lymph nodes using vibrational spectroscopy”, Cranfield University, U. K, August, 2009.
6. External examiner for the degree of Master of Science: “Optical spectroscopy in breast diseases”, Royal Military College of Science, Cranfield University, U. K., 2006.
7. Member of the jury examining the Thesis “Characterisation of the multi-drug resistance (MDR) phenotype in resistant cancer cells by a combined approach based on vibrational spectroscopy and statistical methods”, University of Reims, France, December 2004.

Invited speaker

1. Synchrotron based FTIR spectroscopy. What does the clinician need? IR Workshop on Spectro-Microscopy. asel, witzerland. February, 2011.

2. Synchrotron based FTIR spectroscopy: what does the clinician need? 1st Spanish Workshop on Synchrotron Infrared Microspectroscopy. Madrid, Spain, April, 2008

3. Synchrotron based FITR spectroscopy in the management of cancer. Workshop “Casting New Light on Medicine”. Diamond Light Source. Wellcome Collection Conference Centre, London, March 2008.

4. Synchrotron based FTIR spectroscopy. What does the clinician need? Synchrotron Radiation Users Meeting, September 2007, Diamond Light Source, Oxfordshire, UK. Plenary lecture.
5. Volatile emissions from, lung cancer cells. Breath Analysis in Physiology and Medicine. Prague, Czech Republic, September 2006.
6. Synchrotron based FTIR spectroscopy. What does the clinician expect? Signing of Memorandum of Understanding between Diamond and SOLEIL synchrotrons. British Embassy in Paris, June 2006.
7. Effects of chemotherapy on cancer measured with synchrotron FTIR microspectroscopy. “2nd Diagnostic Applications with Synchrotron Infrared Micro-Spectroscopy (DASIM) Workshop. Frascati, Italy, June 2006.
8. Effects of chemotherapy on cancer cells measured with synchrotron based FTIR microspectroscopy Spec2006. Shedding New Light on Disease: optical diagnosis for the new millennium. Heidelberg, Germany, May 2006.
9. Effects of gemcitabine to in vitro growing lung cancer cells measured with Fourier Transform Infrared Microspectroscopy. Médécine et lumière synchrotron. Institut Curie, Paris, France, December 2005.
10. Effects of gemcitabine to in vitro growing lung cancer cells measured with Fourier Transform Infrared Microspectroscopy. 1st DASIM Workshop, Daresbury Laboratory, Warrington, U. K. July 2005.
11. Spectroscopy, chemotherapy and lung cancer. International workshop on biospectroscopy and chemometrics, Matforsk, Ås, Norway. May, 2004.
12. Volatile emissions from cancer cell lines. Trace Gas Analysis in Medicine, Physiology and Cell Biology. Keele University, Keele, U. K. June, 2003.
13. Application of FTIR spectroscopy in the management of lung cancer. A clinician point of view. Shedding New Light on Disease; optical diagnostics for the new millennium. Reims, France June, 2002.

PhD supervision

Application of vibrational spectroscopy in the management of lung cancer. Mr J. K. Pijanka, 2011.

Press interviews

• “Brightest light in Universe to examine lung cancer cells”, by James Randerson. The Guardian 7th July, 2006.

• “Research at Diamond Synchrotron”, BBC 10 o’clock News (national news), 11^th June, 2010. http://news.bbc.co.uk/1/hi/science_and_environment/10299916.stm.

Science in school program

 Dr Sulé-Suso has been involved with the British Council in a French program to bring Science to schools since 2010.

Interests

Languages:       Catalan, Spanish, English, Italian, and French.