Dr John Glossop

Title: Research Associate
Phone: +44 (0)1782 673812
Email:
Location: Institute for Science and Technology in Medicine, Keele University
Staffordshire Rheumatology Centre, Barker Unit, The Haywood Hospital
High Lane, Burslem, Stoke-on-Trent, Staffordshire, ST6 7AG, UK
Role: ISTM research theme: Clinical & Diagnostic Science
Contacting me: By phone or e-mail please
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Current Position:

Research Associate, 2006 – present

ISTM, Keele University.

Conducting research investigating the role of genetic and epigenetic mechanisms in rheumatoid arthritis pathogenesis.

Education:

Ph.D., Keele University, 2000 – 2005

Thesis title: ”Tumour necrosis factor and its receptors in rheumatoid arthritis: Relationship between gene polymorphism, cytokine production and disease outcome”.

B.Sc., Keele University, 1997 – 2000

Biochemistry and Biology (dual honours), First Class.

ISTM research theme: Clinical & Diagnostic Science

My research focuses on rheumatoid arthritis (RA), a systemic autoimmune disease characterised by chronic inflammation and progressive destruction of the synovial joints. The disease ultimately leads to permanent loss of function and disability. The exact cause of RA is unknown, although it is believed to develop through the complex interaction of various environmental and genetic factors.

A small but growing body of evidence suggests that epigenetic mechanisms that regulate gene expression without altering the underlying DNA sequence may also be important in RA pathogenesis. Currently, I am specifically interested in DNA methylation, one of the principle epigenetic processes. In RA, aberrant methylation may contribute to the persistent over-expression of mediators that promote inflammation, whilst suppressing those which redress inflammation, thereby co-ordinately instructing chronic inflammation and joint destruction. By assessing DNA methylation in multiple cell types using new technologies capable of simultaneously interrogating multiple genes at single nucleotide resolution, this work will define genome-wide cell type-specific DNA methylation signatures for RA. Gene and protein expression analyses being conducted in parallel will engender a more comprehensive understanding of the regulatory actions of DNA methylation in these cells. In concert, the data generated from these studies will be valuable to our understanding of the role of DNA methylation in the pathogenesis and joint destruction of RA.

Glossop JR, Dawes PT, Mattey DL. Antinuclear antibodies are associated with tumor necrosis factor receptor I gene polymorphism in patients with rheumatoid arthritis. Clin. Exp. Rheumatol. 2011. In Press.

Glossop JR, Cartmell SH. Tensile strain and magnetic particle force application do not induce MAP3K8 and IL1B differential gene expression in a similar manner to fluid shear stress in human mesenchymal stem cells. J. Tissue Eng. Regen. Med. 2010;4:577-9.

Glossop JR, Cartmell SH. Differential gene expression of integrins alpha 2 and beta 8 in human mesenchymal stem cells exposed to fluid flow. Cell. Mol. Bioeng. 2009;2:544-53.

Glossop JR, Cartmell SH. Effect of fluid flow-induced shear stress on human mesenchymal stem cells: differential gene expression of IL1B and MAP3K8 in MAPK signaling. Gene Expr. Patterns 2009;9:381-8.

Mattey DL, Glossop JR, Nixon NB, Dawes PT. Circulating levels of tumor necrosis factor receptors are highly predictive of mortality in patients with rheumatoid arthritis. Arthritis Rheum. 2007;56:3940-8.

Glossop JR, Dawes PT, Mattey DL. Association between cigarette smoking and release of tumor necrosis factor alpha and its soluble receptors by peripheral blood mononuclear cells in patients with rheumatoid arthritis. Rheumatology (Oxford) 2006;45:1223-9.

Glossop JR, Dawes PT, Nixon NB, Mattey DL. Polymorphism in the tumor necrosis factor receptor II gene is associated with circulating soluble receptor levels in rheumatoid arthritis. Arthritis Res. Ther. 2005;7:R1227-34.

Glossop JR, Dawes PT, Hassell AB, Mattey DL. Anaemia in rheumatoid arthritis: Association with polymorphism in the tumor necrosis factor receptor I and II genes. J. Rheumatol. 2005;32:1673-8.

Glossop JR, Nixon NB, Dawes PT, Hassell AB, Mattey DL. No association of polymorphisms in the tumor necrosis factor receptor I and receptor II genes with disease severity in rheumatoid arthritis. J. Rheumatol. 2003;30:1406-9.

Glossop JR, et al. 6 published abstracts, 2003-2009.

Selected Publications

  • Glossop JR, Nixon NB, Emes RD, Dawes PT, Farrell WE, Mattey DL. 2013. GENOME-WIDE DNA METHYLATION PROFILING IN T CELLS FROM PATIENTS WITH RHEUMATOID ARTHRITIS. RHEUMATOLOGY (vol. 52, p. 143). link>
  • Rathbone SR, Glossop JR, Gough JE, Cartmell SH. 2012. Cyclic tensile strain upon human mesenchymal stem cells in 2D and 3D culture differentially influences CCNL2, WDR61 and BAHCC1 gene expression levels. J Mech Behav Biomed Mater, vol. 11, 82-91. link> doi>
  • Glossop JR, Hidalgo-Bastida LA, Cartmell SH. 2011. Fluid Shear Stress Induces Differential Gene Expression of Leukemia Inhibitory Factor in Human Mesenchymal Stem Cells. JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, vol. 1(2), 166-176. link> doi>
  • Glossop JR, Dawes PT, Mattey DL. 2011. Antinuclear antibodies are associated with tumor necrosis factor receptor I gene polymorphism in patients with rheumatoid arthritis. Clin Exp Rheumatol, vol. 29(4), 609-615. link>
  • Sura, H, Glossop, JR, Wolbank, S, van Griensven, M, Reidl, H, Dobson, J, El Haj AJ. 2011. An in Vitro model of mesenchymal stem cell targeting using magnetic particle labelling. Journal of Tissue Engineering and Regenerative Medicine. doi>

Full Publications List show

Journal Articles

  • Rathbone SR, Glossop JR, Gough JE, Cartmell SH. 2012. Cyclic tensile strain upon human mesenchymal stem cells in 2D and 3D culture differentially influences CCNL2, WDR61 and BAHCC1 gene expression levels. J Mech Behav Biomed Mater, vol. 11, 82-91. link> doi>
  • Glossop JR, Hidalgo-Bastida LA, Cartmell SH. 2011. Fluid Shear Stress Induces Differential Gene Expression of Leukemia Inhibitory Factor in Human Mesenchymal Stem Cells. JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, vol. 1(2), 166-176. link> doi>
  • Glossop JR, Dawes PT, Mattey DL. 2011. Antinuclear antibodies are associated with tumor necrosis factor receptor I gene polymorphism in patients with rheumatoid arthritis. Clin Exp Rheumatol, vol. 29(4), 609-615. link>
  • Sura, H, Glossop, JR, Wolbank, S, van Griensven, M, Reidl, H, Dobson, J, El Haj AJ. 2011. An in Vitro model of mesenchymal stem cell targeting using magnetic particle labelling. Journal of Tissue Engineering and Regenerative Medicine. doi>
  • Glossop JR and Cartmell SH. 2010. Tensile strain and magnetic particle force application do not induce MAP3K8 and IL-1B differential gene expression in a similar manner to fluid shear stress in human mesenchymal stem cells. J Tissue Eng Regen Med, vol. 4(7), 577-579. link> doi>
  • Glossop JR and Cartmell SH. 2009. Differential Gene Expression of Integrins Alpha 2 and Beta 8 in Human Mesenchymal Stem Cells Exposed to Fluid Flow. CELLULAR AND MOLECULAR BIOENGINEERING, vol. 2(4), 544-553. link> doi>
  • Glossop JR and Cartmell SH. 2009. Effect of fluid flow-induced shear stress on human mesenchymal stem cells: differential gene expression of IL1B and MAP3K8 in MAPK signaling. Gene Expr Patterns, vol. 9(5), 381-388. link> doi>
  • Mattey DL, Glossop JR, Nixon NB, Dawes PT. 2007. Circulating levels of tumor necrosis factor receptors are highly predictive of mortality in patients with rheumatoid arthritis. Arthritis Rheum, vol. 56(12), 3940-3948. link> doi>
  • Glossop JR, Dawes PT, Mattey DL. 2006. Association between cigarette smoking and release of tumour necrosis factor alpha and its soluble receptors by peripheral blood mononuclear cells in patients with rheumatoid arthritis. Rheumatology (Oxford), vol. 45(10), 1223-1229. link> doi> full text>
  • Glossop JR, Dawes PT, Hassell AB, Mattey DL. 2005. Anemia in rheumatoid arthritis: association with polymorphism in the tumor necrosis factor receptor I and II genes. J Rheumatol, vol. 32(9), 1673-1678. link>
  • Glossop JR, Dawes PT, Nixon NB, Mattey DL. 2005. Polymorphism in the tumour necrosis factor receptor II gene is associated with circulating levels of soluble tumour necrosis factor receptors in rheumatoid arthritis. Arthritis Res Ther, vol. 7(6), R1227-R1234. link> doi> full text>
  • Glossop JR, Nixon NB, Dawes PT, Hassell AB, Mattey DL. 2003. No association of polymorphisms in the tumor necrosis factor receptor I and receptor II genes with disease severity in rheumatoid arthritis. J Rheumatol, vol. 30(7), 1406-1409. link>
  • Glossop JR, Nixon NB, Dawes PT, Hassell AB, Mattey DL. 2003. ASSOCIATION OF POLYMORPHISM IN EXON 1 OF THE TUMOR NECROSIS FACTOR RECEPTOR SUPER FAMILY 1A GENE WITH HAEMOGLOBIN LEVELS IN PATIENTS WITH RHEUMATOID ARTHRITIS. RHEUMATOLOGY, vol. 42, 84. link>

Other

  • Glossop JR, Nixon NB, Emes RD, Dawes PT, Farrell WE, Mattey DL. 2013. GENOME-WIDE DNA METHYLATION PROFILING IN T CELLS FROM PATIENTS WITH RHEUMATOID ARTHRITIS. RHEUMATOLOGY (vol. 52, p. 143). link>
  • Glossop JR and Cartmell SH. 2007. Mesenchymal stem cells and tensile strain. TISSUE ENGINEERING (vol. 13, p. 1724). link>
  • Mattey DL, Glossop JR, Nixon NB, Dawes PT. 2006. Circulating levels of tumour necrosis factor receptors are associated with cardiovascular disease in RA independent of activity and severity of rheumatoid disease. ANNALS OF THE RHEUMATIC DISEASES (vol. 65, p. 461). link>
  • Glossop JR, Dawes PT, Mattey DL. 2006. Cigarette smoking and release of TNF alpha and its soluble receptors by peripheral blood mononuclear cells in patients with rheumatoid arthritis. RHEUMATOLOGY (vol. 45, pp. I31-I32). link>
  • Glossop JR, Dawes PT, Hassell AB, Mattey DL. 2005. Association of soluble tumour necrosis factor receptor II levels with mortality in rheumatoid arthritis. RHEUMATOLOGY (vol. 44, pp. I11-I12). link>
  • GLOSSOP JR, DAWES PT, HASSELL AB, MATTEY DL. 2004. Polymorphism in the tumour necrosis factor receptor II gene is associated with the soluble receptor levels in rheumatoid arthritis. Arthritis and Rheumatism.
  • Glossop JR, Dawes PT, Nixon NB, Hassell AB, Mattey DL. 2003. Association between T-cell production of tumor necrosis factor alpha and the presence of nodular disease in rheumatoid arthritis. Annals of the Rheumatic Diseases.

M.Sc. in Cell and Tissue Engineering/Biomedical Engineering, 2006 – present

Module Co-ordinator, ‘Molecular Techniques: Applications in Tissue Engineering’ (2008 – present)

I am responsible for the design, delivery and management of the module. This includes preparing and delivering lectures, a practical demonstration and tutorials, arranging guest lecturers, devising and marking module assessments (essay and exam), and managing content delivery through the online Keele Learning Environment.

Module Lecturer, ‘Cell and Tissue Engineering’ (2006 – present).

Module Lecturer, ‘Molecular Techniques: Applications in Tissue Engineering’ (2006 – 2008).