ISTM
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- Glenn Morris
The main aim of the Group is to produce monoclonal antibodies and use them as highly-specific research tools for analysis of molecular mechanisms in the pathogenesis of human genetic disease and in the search for new therapies. The Research Laboratory interacts closely with consultant pathologist and physician members of the Wolfson Centre.
The Wolfson Centre runs the Muscular Dystrophy Association Monoclonal Antibody Resource (Manager: Dr Le Thanh Lam) which distributes free antibodies for research into neuromuscular disease worldwide (www.glennmorris.org.uk/mabs.htm). The antibodies are widely used in clinical trials of new treatments for Duchenne muscular dystrophy.
ISTM Research theme: 1. Bioengineering & Therapeutics
Research Interests:
• Monoclonal antibody studies of myotonic dystrophy (Dr Ian Holt).
• Monoclonal antibody studies Emery-Dreifuss MD(Dr Ian Holt).
• Proteomic studies of spinal muscular atrophy (Dr. Heidi Fuller).
• Cellular models for McArdle Disease (Dr. Kathryn Wright).
• An SMN ELISA to find new drugs for spinal muscular atrophy (Dr Nguyen thi Man and Dr Emma Humphrey).
• Monoclonal antibody studies of dystrophin and related proteins (Dr. Le Thanh Lam).
Funding:
The Group is entirely funded by medical research charities with a direct research income averaging £150-250K per annum.
Please see: www.glennmorris.org.uk
Selected Publications
Full Publications List show
Journal Articles
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2005. Epitope mapping. Methods Mol Biol, vol. 295, 255-268. link>
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2004. Protein interactions, right or wrong, in Emery-Dreifuss muscular dystrophy. Symp Soc Exp Biol, 57-68. link>
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2001. Absence of utrophin in intercalated discs of human cardiac muscle. NEUROMUSCULAR DISORDERS, vol. 11(6-7), 651. link>
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2001. Skeletal and cardiac muscle pathology in a proven case of autosomal dominant Emery-Dreifuss muscular dystrophy. NEUROMUSCULAR DISORDERS, vol. 11(6-7), 656. link>
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2001. SMN protein studies in spinal muscular atrophy. NEUROMUSCULAR DISORDERS, vol. 11(6-7), 647-648. link>
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2001. Both emerin and lamin C depend on lamin A for localization at the nuclear envelope. JOURNAL OF CELL SCIENCE, vol. 114(14), 2577-2590. link>
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2000. Transcriptional regulation by huntingtin. AMERICAN JOURNAL OF HUMAN GENETICS, vol. 67(4), 365. link>
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2000. New nomenclature and DNA testing guidelines for myotonic dystrophy type 1(DM1). NEUROLOGY, vol. 54(6), 1218-1221. link>
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1999. Emerin is associated with lamin A/C in one subset of kidney cell nuclei while lamin B1 is present in the emerin-negative nuclei. AMERICAN JOURNAL OF HUMAN GENETICS, vol. 65(4), A478. link>
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1999. Is the myotonic dystrophy protein kinase (DMPK) a muscle-specific protein?. AMERICAN JOURNAL OF HUMAN GENETICS, vol. 65(4), A279. link>
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1999. The spinal muscular atrophy protein, SMN, fails to form nuclear gems in some mammalian cell types which also lack coiled bodies. AMERICAN JOURNAL OF HUMAN GENETICS, vol. 65(4), A114. link>
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1998. Full length huntingtin is not detected in intranuclear inclusions in Huntington's disease brain. EUROPEAN JOURNAL OF HUMAN GENETICS, vol. 6, 150. link>
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1997. Structural studies of dystrophin and utrophin using monoclonal antibodies. FASEB JOURNAL, vol. 11(9), A1213. link>
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1997. Monoclonal antibodies from three new regions of huntingtin, the Huntington's disease protein. Biochem Soc Trans, vol. 25(1), 51S. link>
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1997. Nucleotide sequences from the capsid and pre-protein regions of dengue viruses from VietNam. Biochem Soc Trans, vol. 25(1), 54S. link>
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1997. Protease digestion studies of an equilibrium intermediate in the unfolding of creatine kinase. Biochem J, vol. 321 ( Pt 1), 83-88. link>
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1995. Evidence for a utrophin-glycoprotein complex in cultured cell lines and a possible role in cell adhesion. Biochem Soc Trans, vol. 23(3), 398S. link>
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1995. Probing protein structure with proteases: studies of an equilibrium intermediate in protein unfolding. Biochem Soc Trans, vol. 23(3), 477S. link>
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1995. Specificity and VH sequence of two monoclonal antibodies against the N-terminus of dystrophin. Biochem J, vol. 309 ( Pt 1), 355-359. link>
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1995. Characterization of revertant muscle fibers in Duchenne muscular dystrophy, using exon-specific monoclonal antibodies against dystrophin. Am J Hum Genet, vol. 56(3), 725-731. link>
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1995. Full-length and short forms of utrophin, the dystrophin-related protein. FEBS Lett, vol. 358(3), 262-266. link>
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1994. APO-DYSTROPHIN-1 AND APO-DYSTROPHIN-2, PRODUCTS OF THE DUCHENNE MUSCULAR-DYSTROPHY LOCUS - EXPRESSION DURING MOUSE EMBRYOGENESIS AND IN CULTURED-CELL LINES. HUMAN MOLECULAR GENETICS, vol. 3(8), 1309-1316. link>
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1993. DYSTROPHIN-RELATED PROTEIN, UTROPHIN, IN NORMAL AND DYSTROPHIC HUMAN FETAL SKELETAL-MUSCLE. HISTOCHEMICAL JOURNAL, vol. 25(8), 554-561. link>
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1993. Monoclonal antibodies against the muscle-specific N-terminus of dystrophin: characterization of dystrophin in a muscular dystrophy patient with a frameshift deletion of exons 3-7. Am J Hum Genet, vol. 53(1), 131-139. link>
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1993. Use of epitope libraries to identify exon-specific monoclonal antibodies for characterization of altered dystrophins in muscular dystrophy. Am J Hum Genet, vol. 52(6), 1057-1066. link>
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1992. COLOCALIZATION AND MOLECULAR ASSOCIATION OF DYSTROPHIN WITH LAMININ AT THE SURFACE OF MOUSE AND HUMAN MYOTUBES. JOURNAL OF CELL SCIENCE, vol. 103, 1223-&. link>
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1992. Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections. Biochem J, vol. 288 ( Pt 2), 663-668. link>
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1992. Utrophin, the autosomal homologue of dystrophin, is widely-expressed and membrane-associated in cultured cell lines. FEBS Lett, vol. 313(1), 19-22. link>
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1992. A TRUNCATED DYSTROPHIN LACKING THE C-TERMINAL DOMAINS IS LOCALIZED AT THE MUSCLE MEMBRANE. AMERICAN JOURNAL OF HUMAN GENETICS, vol. 50(3), 508-514. link>
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1991. Identification by protein microsequencing of a proteinase-V8-cleavage site in a folding intermediate of chick muscle creatine kinase. Biochem J, vol. 280 ( Pt 3), 809-811. link>
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1991. Localization of the DMDL gene-encoded dystrophin-related protein using a panel of nineteen monoclonal antibodies: presence at neuromuscular junctions, in the sarcolemma of dystrophic skeletal muscle, in vascular and other smooth muscles, and in proliferating brain cell lines. J Cell Biol, vol. 115(6), 1695-1700. link>
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1991. SEQUENCE EVOLUTION AND TISSUE EXPRESSION OF THE DMD-LIKE LOCUS (DMDL) ON CHROMOSOME 6. AMERICAN JOURNAL OF HUMAN GENETICS, vol. 49(4), 420. link>
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1991. Structural changes in the C-terminal region of human brain creatine kinase studied with monoclonal antibodies. Biochim Biophys Acta, vol. 1076(2), 245-251. link>
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1989. Treatment of human muscle creatine kinase with glutaraldehyde preferentially increases the immunogenicity of the native conformation and permits production of high-affinity monoclonal antibodies which recognize two distinct surface epitopes. J Immunol Methods, vol. 125(1-2), 251-259. link> doi>
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1989. Monoclonal antibody studies of creatine kinase. The ART epitope: evidence for an intermediate in protein folding. Biochem J, vol. 257(2), 461-469. link>
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1988. IDENTIFICATION OF C-TERMINAL EPITOPES ON CREATINE-KINASE USING MONOCLONAL-ANTIBODIES. BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 16(5), 717-718. link>
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1987. Monoclonal antibody studies of creatine kinase. Antibody-binding sites in the N-terminal region of creatine kinase and effects of antibody on enzyme refolding. Biochem J, vol. 248(1), 53-59. link>
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1986. MONOCLONAL-ANTIBODY STUDIES OF CREATINE-KINASE - CROSS-REACTION BETWEEN CHICKEN AND HUMAN ENZYMES. BIOCHEMICAL SOCIETY TRANSACTIONS, vol. 14(2), 304. link>
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1986. MONOCLONAL-ANTIBODIES AS PROBES OF CREATINE-KINASE STRUCTURE AND FUNCTION. MUSCLE & NERVE, vol. 9(5), 175. link>
Other
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2005. A monoclonal antibody study of the role of the SMN complex in spinal muscular atrophy. NEUROMUSCULAR DISORDERS (vol. 15, p. 725). link>
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2005. An ELISA assay for measuring the survival of motor neurons (SMN) protein and the search for a drug treatment for spinal muscular atrophy. NEUROMUSCULAR DISORDERS (vol. 15, p. 726). link>
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2004. Localisation and tissue distribution of the three human muscleblind proteins associated with expanded repeats in myotonic dystrophy. NEUROMUSCULAR DISORDERS (vol. 14, p. 585). link>
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2004. One hundred and fifty monoclonal antibodies against dystrophin and their application to studies of pathogenesis and therapy in Duchenne muscular dystrophy. NEUROMUSCULAR DISORDERS (vol. 14, p. 578). link>
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2004. Towards a treatment for spinal muscular atrophy: looking for drugs that increase SMN protein levels. NEUROMUSCULAR DISORDERS (vol. 14, pp. 595-596). link>
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2004. Use of antisense-mediated exon skipping to generate dystrophin in muscles of the mdx dystrophic mouse. NEUROMUSCULAR DISORDERS (vol. 14, p. 624). link>
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2003. Rapid identification of novel canine models of Duchenne muscular dystrophy. MOLECULAR THERAPY (vol. 7, p. S101). link>
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2003. Results of phase I clinical trial of plasmid-dystrophin intramuscular administration into Duchenne and Becker dystrophy patients. MOLECULAR THERAPY (vol. 7, p. S449). link>
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1998. Flexible hinges in dystrophin. Biochem Soc Trans (vol. 26, p. S310). link>
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1998. Full length huntingtin is not detected in intranuclear inclusions in Huntington's disease brain. Biochem Soc Trans (vol. 26, p. S243). link>
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1995. EVIDENCE FOR A UTROPHIN-GLYCOPROTEIN COMPLEX IN CULTURED-CELL LINES AND A POSSIBLE ROLE IN CELL-ADHESION. BIOCHEMICAL SOCIETY TRANSACTIONS (vol. 23, p. S398). link>
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1995. PROBING PROTEIN-STRUCTURE WITH PROTEASES - STUDIES OF AN EQUILIBRIUM INTERMEDIATE IN PROTEIN UNFOLDING. BIOCHEMICAL SOCIETY TRANSACTIONS (vol. 23, p. S477). link>
Personal website: www.glennmorris.org.uk
Check out our NEW monoclonal antibody website at: http://glennmorris.org.uk/mabs.htm
Professor Morris is on the Editorial Boards of:
- “Neuromuscular Disorders”
- “Translational Neuroscience”

