Biography
I was appointed to a personal Chair in Biophysics in 2004. Prior to that I was a Lecturer and then Senior Lecturer in Physics at Keele. Since 2000 I have been on secondment to the Partnership for Structural Biology at the Institut Laue Langevin (ILL) in Grenoble, France, where I hold a Senior Fellowship and am Head of the Life Sciences Group.
Research and scholarship
The main research interests of my group concern the study of macromolecular systems using physical techniques. Neutron and X-ray scattering techniques at the Institut Laue Langevin (ILL) and the European Synchrotron Radiation Facility (ESRF) are central to this. Current project work includes structural studies of DNA, DNA-drug interactions and DNA-protein complexes, self assembling systems of synthetic and biological origin.
We are also deeply involved in methodological developments for neutron and X-ray scattering. The Integrated Macromolecular Structure Research Group is based at the Partnership for Structural Biology (PSB) in Grenoble, France, where a wide variety of techniques are available to complement the capabilities of the Grenoble central facility infrastructures. For further details, see (http://www.ill.fr/deuteration).
Selected Publications
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Structure and diffusive dynamics of aspartate α-decarboxylase (ADC) liganded with D-serine in aqueous solution. Phys Chem Chem Phys, 20336-20347, vol. 24(34). link> doi> full text>2022.
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Microgravity crystallization of perdeuterated tryptophan synthase for neutron diffraction. NPJ Microgravity, 13, vol. 8(1). link> doi> full text>2022.
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Nanoscale Structure and Dynamics of Model Membrane Lipid Raft Systems, Studied by Neutron Scattering Methods. Frontiers in Physics, vol. 10. link> doi> link> full text>2022.
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Neutron crystallography reveals mechanisms used by Pseudomonas aeruginosa for host-cell binding. Nature Communications, Article 194, vol. 13(1). link> doi> link> full text>2022.
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Cryo-EM structure of MsbA in saposin-lipid nanoparticles (Salipro) provides insights into nucleotide coordination. FEBS J, 2959-2970, vol. 289(10). link> doi> full text>2022.
Full Publications Listshow
Journal Articles
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Structure and diffusive dynamics of aspartate α-decarboxylase (ADC) liganded with D-serine in aqueous solution. Phys Chem Chem Phys, 20336-20347, vol. 24(34). link> doi> full text>2022.
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Microgravity crystallization of perdeuterated tryptophan synthase for neutron diffraction. NPJ Microgravity, 13, vol. 8(1). link> doi> full text>2022.
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Nanoscale Structure and Dynamics of Model Membrane Lipid Raft Systems, Studied by Neutron Scattering Methods. Frontiers in Physics, vol. 10. link> doi> link> full text>2022.
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Neutron crystallography reveals mechanisms used by Pseudomonas aeruginosa for host-cell binding. Nature Communications, Article 194, vol. 13(1). link> doi> link> full text>2022.
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Cryo-EM structure of MsbA in saposin-lipid nanoparticles (Salipro) provides insights into nucleotide coordination. FEBS J, 2959-2970, vol. 289(10). link> doi> full text>2022.
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The impact of folding modes and deuteration on the atomic resolution structure of hen egg-white lysozyme. Acta Crystallogr D Struct Biol, 1579-1590, vol. 77(Pt 12). link> doi> full text>2021.
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Neutron crystallography reveals novel mechanisms used by <i>Pseudomonas aeruginosa</i> for host-cell binding. doi>2021.
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Visualization of hydrogen atoms in a perdeuterated lectin-fucose complex reveals key details of protein-carbohydrate interactions. Structure, 1003-1013.e4, vol. 29(9). link> doi> full text>2021.
- 2021.
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Metabolic fluxes for nutritional flexibility of Mycobacterium tuberculosis. Mol Syst Biol, e10280, vol. 17(5). link> doi> full text>2021.
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ApoE and ApoE Nascent-Like HDL Particles at Model Cellular Membranes: Effect of Protein Isoform and Membrane Composition. Front Chem, 630152, vol. 9. link> doi> full text>2021.
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Apolipoprotein E Binding Drives Structural and Compositional Rearrangement of mRNA-Containing Lipid Nanoparticles. ACS Nano, 6709-6722, vol. 15(4). link> doi> full text>2021.
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Structural insights into protein folding, stability and activity using in vivo perdeuteration of hen egg-white lysozyme. IUCrJ, 372-386, vol. 8(Pt 3). link> doi> full text>2021.
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A Dimerization Site at SCR-17/18 in Factor H Clarifies a New Mechanism for Complement Regulatory Control. Front Immunol, 601895, vol. 11. link> doi> full text>2020.
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Nanostructural deformation of high-stiffness spruce wood under tension. Sci Rep, 453, vol. 11(1). link> doi> full text>2021.
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Elongation rate and average length of amyloid fibrils in solution using isotope-labelled small-angle neutron scattering. RSC Chemical Biology. doi> link> full text>2021.
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Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 158769, vol. 1865(10). link> doi> link> full text>2020.
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Thermal Neutron Relative Biological Effectiveness Factors for Boron Neutron Capture Therapy from In Vitro Irradiations. Cells, vol. 9(10). link> doi> full text>2020.
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Radiobiology data of melanoma cells after low-energy neutron irradiation and boron compound administration. Applied Radiation and Isotopes, Article 109205, vol. 163. link> doi> link> full text>2020.
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Diffusive dynamics of Aspartate<i>α</i>-decarboxylase (ADC) liganded with D-serine in aqueous solution. doi>2020.
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Production of perdeuterated fucose from glyco-engineered bacteria. Glycobiology. link> doi> link> full text>2020.
- 2020.
- 2020.
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Towards a molecular understanding of the water purification properties of Moringa seed proteins. J Colloid Interface Sci, 296-304, vol. 554. link> doi> full text>2019.
- 2019.
- 2019.
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In Vivo Water Dynamics in Shewanella oneidensis Bacteria at High Pressure. Sci Rep, 8716, vol. 9(1). link> doi> full text>2019.
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Time-resolved small-angle neutron scattering as a probe for the dynamics of lipid exchange between human lipoproteins and naturally derived membranes. Scientific Reports, Article 7591, vol. 9(1). link> doi> link> full text>2019.
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Protein Short-Time Diffusion in a Naturally Crowded Environment. Journal of Physical Chemistry Letters, 1709-1715, vol. 10(8). link> doi> link> full text>2019.
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The Production of Matchout-Deuterated Cholesterol and the Study of Bilayer-Cholesterol Interactions. Scientific Reports, 5118, vol. 9(1). link> doi> link> full text>2019.
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A molecular mechanism for transthyretin amyloidogenesis. Nature Communications, 925, vol. 10(1). link> doi> link> full text>2019.
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Deuterium effects on human serum albumin in solution. PHYSICA B-CONDENSED MATTER, 208-211, vol. 551. link> doi> full text>2018.
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Neutron scattering for the study of biological systems - major opportunities within a rapidly changing landscape. Acta Crystallogr D Struct Biol, 1126-1128, vol. 74(Pt 12). link> doi> full text>2018.
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Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Commun Biol, 206, vol. 1. link> doi> full text>2018.
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Structural basis for activation of plasma-membrane Ca2+-ATPase by calmodulin. Communications Biology, 206, vol. 1(1). link> doi> link> full text>2018.
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Conformational States of ABC Transporter MsbA in a Lipid Environment Investigated by Small-Angle Scattering Using Stealth Carrier Nanodiscs. Structure, 1072-1079.e4, vol. 26(8). link> doi> full text>2018.
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A novel amyloid designable scaffold and potential inhibitor inspired by GAIIG of amyloid beta and the HIV-1 V3 loop. FEBS Lett, 1777-1788, vol. 592(11). link> doi> full text>2018.
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Adsorption of Denaturated Lysozyme at the Air-Water Interface: Structure and Morphology. Langmuir, 5020-5029, vol. 34(17). link> doi> full text>2018.
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Dynamic self-assembly of DNA minor groove-binding ligand DB921 into nanotubes triggered by an alkali halide. Nanoscale, 5550-5558, vol. 10(12). link> doi> full text>2018.
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Perdeuteration of cholesterol for neutron scattering applications using recombinant Pichia pastoris. Chem Phys Lipids, 80-87, vol. 212. link> doi> full text>2018.
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Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine. Langmuir, 472-479, vol. 34(1). link> doi> link> full text>2018.
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Analysis of XFEL serial diffraction data from individual crystalline fibrils. IUCrJ, 795-811, vol. 4(Pt 6). link> doi> full text>2017.
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In vivo analysis of the Escherichia coli ultrastructure by small-angle scattering. IUCrJ, 751-757, vol. 4(Pt 6). link> doi> full text>2017.
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Laser processing of protein films as a method for accomplishment of cell patterning at the microscale. Biofabrication, 045004, vol. 9(4). link> doi> full text>2017.
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Self-Assembled Amyloid Peptides with Arg-Gly-Asp (RGD) Motifs As Scaffolds for Tissue Engineering. ACS BIOMATERIALS SCIENCE & ENGINEERING, 1404-1416, vol. 3(7). link> doi> link> full text>2017.
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Flow-aligned, single-shot fiber diffraction using a femtosecond X-ray free-electron laser. Cytoskeleton (Hoboken), 472-481, vol. 74(12). link> doi> link> full text>2017.
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Back-exchange of deuterium in neutron crystallography: characterization by IR spectroscopy. J Appl Crystallogr, 660-664, vol. 50(Pt 2). link> doi> full text>2017.
- 2017.
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SEC-SANS: size exclusion chromatography combined in situ with small-angle neutron scattering. Journal of Applied Crystallography, 2015-2020, vol. 49(Pt 6). link> doi> full text>2016.
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Matchout deuterium labelling of proteins for small-angle neutron scattering studies using prokaryotic and eukaryotic expression systems and high cell-density culture. European Biophysics Journal, 425-432, vol. 46(5). doi> link> full text>2016.
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Matchout deuterium labelling of proteins for small-angle neutron scattering studies using prokaryotic and eukaryotic expression systems and high cell-density cultures. European Biophysics Journal. link> doi> link> full text>
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Macromolecular structure phasing by neutron anomalous diffraction. SCIENTIFIC REPORTS, Article ARTN 31487, vol. 6. link> doi> link> full text>2016.
- 2016.
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Impact of Deuteration on the Assembly Kinetics of Transthyretin Monitored by Native Mass Spectrometry and Implications for Amyloidoses. Angewandte Chemie (International ed. in English). doi> link> full text>2016.
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Water Dynamics in Shewanella oneidensis at Ambient and High Pressure using Quasi-Elastic Neutron Scattering. Sci Rep, 18862, vol. 6. link> doi> full text>2016.
- 2016.
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Neutron reflection study of the interaction of the eukaryotic pore-forming actinoporin equinatoxin II with lipid membranes reveals intermediate states in pore formation. Biochimica et Biophysica Acta - Biomembranes. doi> full text>2015.
- 2015.
- 2015.
- 2015.
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The pentameric nucleoplasmin fold is present in Drosophila FKBP39 and a large number of chromatin-related proteins. J Mol Biol, 1949-1963, vol. 427(10). link> doi> full text>2015.
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Diffraction evidence for the structure of cellulose microfibrils in bamboo, a model for grass and cereal celluloses. BMC Plant Biol, 153, vol. 15. link> doi> full text>2015.
- 2015.
- 2014.
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Binding site asymmetry in human transthyretin: insights from a joint neutron and X-ray crystallographic analysis using perdeuterated protein. IUCrJ, 429-438, vol. 1(Pt 6). link> doi> full text>2014.
- 2014.
- 2014.
- 2014.
- 2014.
- 2014.
- 2014.
- 2014.
- 2013.
- 2013.
- 2013.
- 2013.
- 2013.
- 2013.
- 2012.
- 2012.
- 2012.
- 2012.
- 2011.
- 2011.
- 2011.
- 2011.
- 2011.
- 2011.
- 2011.
- 2011.
- 2010.
- 2010.
- 2010.
- 2010.
- 2010.
- 2010.
- 2010.
- 2010.
- 2004.
- 2009.
- 2009.
- 2008.
- 2008.
- 2008.
- 2008.
- 2008.
- 2008.
- 2007.
- 2006.
- 2006.
- 2005.
- 2005.
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Amyloid fibril formation from sequences of a natural beta-structured fibrous protein, the adenovirus fiber. Journal of Biological Chemistry, 2481-2490, vol. 280. doi>2005.
- 2004.
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New Insights into the Structure of Poly(p-phenylene terephthalamide) from Neutron Fiber Diffraction Studies. Macromolecules, 9654-9656, vol. 37(25). doi>2004.
- 2004.
- 2004.
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Water-DNA interactions as studied by X-ray and neutron fibre diffraction. Philosophical Transactions of the Royal Society of London.2004.
- 2003.
- 2003.
- 2002.
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Folding of a fibrous protein and its self-assembling peptides. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, D30, vol. 223. link>2002.
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Structural studies of biological & synthetic polymers using x-ray and neutron diffraction. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, D26, vol. 223. link>2002.
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Neutron diffraction study of a phenol-nitroxide radical adduct: A structural model for hydrogen atom abstraction by peroxyl radicals from vitamin E and related phenolic antioxidants. Journal of the American Chemical Society, 9164-9165, vol. 123. doi>2001.
- 2000.
- 1998.
- 1998.
- 1997.
- 1995.
- 1994.
- 1992.
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TIME-RESOLVED X-RAY FIBER DIFFRACTION STUDIES OF THE S-REVERSIBLE-B CONFORMATIONAL TRANSITION IN THE POLY [D(G-C)] POLY [D(G-C)] DOUBLE HELIX. INSTITUTE OF PHYSICS CONFERENCE SERIES, 225-236. link>1990.
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X-RAY AND NEUTRON HIGH ANGLE FIBER DIFFRACTION STUDIES IN THE DETERMINATION OF HYDRATION AND IONIC STRUCTURE AROUND DNA. INSTITUTE OF PHYSICS CONFERENCE SERIES, 237-248. link>1990.
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X-RAY AND NEUTRON-DIFFRACTION STUDIES OF THE STRUCTURE OF PEEK. INSTITUTE OF PHYSICS CONFERENCE SERIES, 213-224. link>1990.
- 1989.
- 1989.
- 1988.
- 1986.
- 1986.
Chapters
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Nanoparticle Characterisation Methods: Applications of Synchrotron and Neutron Radiation. In Pharmaceutical Nanotechnology: Innovation and Production. Wiley. doi>
Other
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Special Issue: Polymer-Solvent Complexes and Intercalates POLYSOLVAT-12 Preface. Macromolecular Symposia (vol. 386). Wiley-VCH Verlag. link> doi> full text>2019.
- 2018.
- 2017.
- 2017.
- 2017.
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Lipoprotein structure dependency on lipid cargo and exchange dynamics: Implications for atherosclerosis development. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY (vol. 253). link>2017.
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Neutron and x-ray scattering approaches for interdisciplinary structural biology. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY (vol. 253). link>2017.
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Orientation and analysis of XFEL serial diffraction patterns from fibrous molecular assemblies. 2017 INTERNATIONAL CONFERENCE ON IMAGE AND VISION COMPUTING NEW ZEALAND (IVCNZ). link>2017.
- 2016.
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A dimerization site at SCR-17/18 in Factor H may explain its disease-causing mutations and a new mechanism for regulatory control. MOLECULAR IMMUNOLOGY (p. 136, vol. 67). link>2015.
- 2014.
- 2014.
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Crystalline transition mechanism by amine molecules during complexation process: Crystal structure of polysaccharides-amine complexes. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY (vol. 247). link>2014.
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Charaterising PC/cholesterol supported lipid bilayers and interactions with human HDL. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. C105, vol. 70). link>2014.
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Structural studies of dynamic CD4 changes relevant to HIV infection. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. C540, vol. 70). link>2014.
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Unravelling transthyretin amyloidosis by neutron crystallography. ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (p. C646, vol. 70). link>2014.
- 2012.
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Hydrogen bonding in anhydrous and dihydrate form of beta-chitin from X-ray and neutron fiber diffraction analysis. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY (vol. 243). link>2012.
- 2011.
- 2011.
- 2011.
- 2011.
- 2011.
- 2011.
- 2009.
- 2009.
- 2008.
- 2008.
- 2008.
- 2007.
- 2007.
- 2006.
- 2006.
- 2006.
- 2005.
- 2005.
- 2005.
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The mechanical role of intermediate filaments in cells: Insights from hagfish slime threads. BIOPHYSICAL JOURNAL (p. 440A, vol. 84). link>2003.
- 1997.
- 1997.
- 1996.
- 1996.
- 1995.
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COMPLEMENTARY X-RAY AND NEUTRON FIBER DIFFRACTION STUDIES OF THE DISTRIBUTION OF WATER AND CATIONS AROUND THE A-DNA DOUBLE HELIX. WATER-BIOMOLECULE INTERACTIONS (pp. 235-238, vol. 43). link>1993.
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THE LOCATION OF IONS AND WATER AROUND THE D CONFORMATION OF DNA. WATER-BIOMOLECULE INTERACTIONS (pp. 231-234, vol. 43). link>1993.
- 1992.
- 1992.
- 1992.
- 1992.
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TIME-RESOLVED X-RAY FIBER DIFFRACTION STUDIES OF THE S-REVERSIBLE-B CONFORMATIONAL TRANSITION IN THE POLY [D(G-C)] POLY [D(G-C)] DOUBLE HELIX. NEUTRON AND X-RAY SCATTERING : COMPLEMENTARY TECHNIQUES (pp. 225-236, vol. 101). link>1990.
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X-RAY AND NEUTRON HIGH ANGLE FIBER DIFFRACTION STUDIES IN THE DETERMINATION OF HYDRATION AND IONIC STRUCTURE AROUND DNA. NEUTRON AND X-RAY SCATTERING : COMPLEMENTARY TECHNIQUES (pp. 237-248, vol. 101). link>1990.
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X-RAY AND NEUTRON-DIFFRACTION STUDIES OF THE STRUCTURE OF PEEK. NEUTRON AND X-RAY SCATTERING : COMPLEMENTARY TECHNIQUES (pp. 213-224, vol. 101). link>1990.
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Lipid dynamics in raft domains and effect of an oxidized bilayer component on their formation. RAW data collected at the facility during an experiment.doi>
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Lipid dynamics in raft domains and effect of an oxidized bilayer component on their formation. RAW data collected at the facility during an experiment.doi>
School address
Lennard-Jones School of Chemical and Physical Sciences
Lennard-Jones Building
Keele University
Staffordshire
ST5 5BG, UK
Phone (School Office): +44 (0)1782 733033, +44 (0)1782 733313
Email: chemistry@keele.ac.uk, forensics@keele.ac.uk, physics@keele.ac.uk
Programme directors
Chemistry and Medicinal Chemistry
Dr Matthew O'Brien
Tel : +44 (0)1782 734371
Email : m.obrien@keele.ac.uk
Forensic Science
Dr Jamie K. Pringle
Tel : +44 (0)1782 733163
Email : j.k.pringle@keele.ac.uk
Physics and Astrophysics
Dr Arumugam Mahendrasingam
Tel : +44 (0)1782 733312
Email : a.mahendrasingam@keele.ac.uk
Admission tutors
Chemistry and Medicinal Chemistry
Dr Natalie Capel
Tel : +44 (0)1782 733584
Email : n.j.capel@keele.ac.uk
Forensic Science
Victoria Cartwright
Tel : +44 (0)1782 733584
Email : v.cartwright@keele.ac.uk
Physics and Astrophysics
Dr Patrick Connell
Tel : +44 (0)1782 733892
Email : physics@keele.ac.uk or p.j.connell@keele.ac.uk