Techniques

Light microscopy

• paraffin or resin embedding for light microscopy
• sectioning (thick or semi-thin)
• standard anatomical and histochemical staining
• immunofluorescence or immunoperoxidase staining
• bright field, phase contrast or Nomarski DIC

Confocal and two-photon imaging

• imaging of sections, cell cultures or whole mounts
• immunofluorescence
• 3-D image stacking and processing
• in vitro vital dye imaging with time-course analysis

Conventional transmission electron microscopy

• resin embedding for TEM
• sectioning (semi-thin or ultrathin )
• standard ultrastructural staining
• negative staining
• quantitative and semi-quantitative immunogold labelling using antibodies
• stereological analysis

High resolution scanning electron microscopy

• sample dehydration (critical point drying)
• sample coating – sputter coating with gold or OTOTO
• wet sample imaging
• high resolution imaging ~ 1.5 nm
• high depth-of-field low power imaging
• backscattered electron imaging

Conventional and digital imaging

• all confocal, LM, SEM and TEM images can be acquired digitally
• conventional photography, photographic development and printing available
• software available for image quantification and analysis
• slide maker available for 35 mm slides (from PowerPoint)