Dr Srabasti Jayanti Chakravorty

Title: Lecturer
Phone: +44 (0)1782 733685
Email: s.j.chakravorty@keele.ac.uk
Location: Huxley Building : H102b
Role: Course Director (Human Biology); First Year Tutor (Human Biology), Admissions Tutor (Human Biology); Staff Link for Student-Staff Liason Committee.
Contacting me: Enquire at reception or arrange appointment by email
srabasti chakravorty

I was appointed as a Lecturer at the School of Life Sciences at Keele University in October 2007.  I graduated with a BSc Honours from the University of Salford before embarking on a research career driven by my interest in the role of the vascular endothelium in pathology.

My career started as Research Assistant at University of Wales College of Medicine at Cardiff before I joined Professor Savage’s group at the University of Birmingham.  Here, I completed a PhD in Medicine, studying the mechanisms of leukocyte recruitment and retention in renal inflammation.  I continued in Professor Savage’s group as a post-doctoral scientist investigating the role of chemokines and adhesion molecules in renal inflammation.  I went on to investigate mechanisms of neutrophil transmigration through endothelial cell monolayers under laminar flow in Professor Gerard Nash’s group, also at the University of Birmingham.

In 2002, I turned my focus on the role of the endothelium in Malaria pathogenesis and joined Professor Alister Craig’s group at the Liverpool School of Tropical Medicine.  Here, I investigated mechanisms of malarial cytoadhesion to the endothelium in vitro, under static and also under laminar flow conditions, leading to a number of successful collaborations.

I completed a Teaching and Learning in Higher Education Postgraduate at Keele University and have achieved recognition as a Fellow of The Higher Education Academy.

I am currently an Editor for Microbes and Infection and have reviewed manuscripts for a number of journals including Malaria Journal, The Journal of Infectious Diseases, PLoS ONE and Microbes and Infection.

I am also the Programme Director for the undergraduate Human Biology Programme at Keele University.  In addition, I have a role as an external examiner for the Human Biology Programme at the University of Derby.  I am serving as a committee member for The Society for the Study of Human Biology.

Broadly my research interests include on the post-adhesive, phenotypic, functional and transcriptional changes in endothelial cells, induced by Plasmodium falciparum-infected red blood cells, in severe malaria.  My research laboratory is established within the Faculty of Natural Sciences in the Centre for Applied Entomology and Parasitology.

 My current research focuses on cerebral malaria, with a view to understanding the mechanisms underlying the disruption of the blood-brain barrier (BBB) integrity and its impact on other cells of the brain parenchyma, such as astrocytes, during sequestration.  The studies in my laboratory involve in vitro co-culture models that mimic sequestration of Plasmodium falciparum-infected red blood cells on the microvascular endothelium of cerebral vessels.  Current investigations using human brain microvascular endothelial cells and human astrocytes in a tandem model system, in vitro, include, 1)functional assays to measure barrier electrical resistance and permeability changes to monitor alterations in the BBB and 2) immunoassays including ELISA, western blotting, immunofluorescence and flow cytometry, to monitor molecular changes in endothelial cells and astrocytes of the BBB.

Previous studies involved microarray analysis of the endothelial cell transcriptome and resulted in a successful collaboration with the Wellcome Trust Sanger Institute, as part of the Wellcome Trust Functional Genomics Initiative in Malaria at Liverpool School of Tropical medicine.

My research at Keele University has been supported by awards from the North Staffordshire Medical Institute, The Wingate Foundation and Institute for Science and Technology in Medicine and the Faculty of Natural Sciences, at Keele University. 

PhD students:

Principal supervisor to: Mohd Hamzah Mohd Nasir (co-supervisor: Prof. Gwyn Williams).  Completed March 2015

Principal supervisor to: Nana Efua Andoh (co-supervisor: Prof. Rosemary Fricker).  In Progress – 2nd year of PhD

Co-supervisor to: Emma Shardlow (Principal supervisor: Prof Christopher Exley).  In Progress - Final year of PhD

Selected Publications

  • Maier AG, Rug M, O'Neill MT, Chakravorty SJ, Szestak T, Chesson J, Wu Y, Hughes K, Coppel RL, Newbold C, Beeson JG, Craig AG, Crabb BS, Cowman AF. 2008. Exported proteins required for virulence and rigidity of Plasmodium falciparium-infected human erythrocytes. CELL, vol. 134, 48-61. doi> link>
  • Grab DJ, Chakravorty SJ, van der Heyde H, Stins MF. 2011. How can microbial interactions with the blood-brain barrier modulate astroglial and neuronal function?. Cell Microbiol, vol. 13(10), 1470-1478. link> doi>
  • Chakravorty SJ, Hughes KR, Craig AG. 2008. Host response to cytoadherence in Plasmodium falciparum. Biochem Soc Trans, vol. 36(Pt 2), 221-228. link> doi>
  • Jenkins N, Wu Y, Chakravorty S, Kai O, Marsh K, Craig A. 2008. Plasmodium falciparum Icam-1-based cytoadherence-related signalling in endothelical cells. JOURNAL OF INFECTION, vol. 56(4), 299. link> doi>
  • Bridges DJ, Chakravorty SJ, Bunn J, O'Donnell J, Grau GE, de laat B, van Mourik JA, Craig A. 2008. The role of von Willebrand factor in malaria pathogenesis. INTERNATIONAL JOURNAL FOR PARASITOLOGY (vol. 38, p. S65). link>

Full Publications List show

Journal Articles

  • Grab DJ, Chakravorty SJ, van der Heyde H, Stins MF. 2011. How can microbial interactions with the blood-brain barrier modulate astroglial and neuronal function?. Cell Microbiol, vol. 13(10), 1470-1478. link> doi>
  • Maier AG, Rug M, O'Neill MT, Chakravorty SJ, Szestak T, Chesson J, Wu Y, Hughes K, Coppel RL, Newbold C, Beeson JG, Craig AG, Crabb BS, Cowman AF. 2008. Exported proteins required for virulence and rigidity of Plasmodium falciparium-infected human erythrocytes. CELL, vol. 134, 48-61. doi> link>
  • Chakravorty SJ, Hughes KR, Craig AG. 2008. Host response to cytoadherence in Plasmodium falciparum. Biochem Soc Trans, vol. 36(Pt 2), 221-228. link> doi>
  • Jenkins N, Wu Y, Chakravorty S, Kai O, Marsh K, Craig A. 2008. Plasmodium falciparum Icam-1-based cytoadherence-related signalling in endothelical cells. JOURNAL OF INFECTION, vol. 56(4), 299. link> doi>
  • CHAKRAVORTY SJ, Carret C, Ivens A, Nash GB. 2007. Altered phenotype and gene transcription in endothelial cells, induced by Plasmodium falciparum-infected red blood cells: Pathogenic or protective?. International Journal for Parasitology , vol. 37(8-9), 975-987. doi>
  • CHAKRAVORTY SJ, Jenkins N, Wu Y, Kai O, Marsh K, Craig A. 2007. Plasmodium falciparum Intercellular Adhesion Molecule–1–Based Cytoadherence‐Related Signaling in Human Endothelial Cells. The Journal of Infectious Diseases, vol. 196(2), 321-327. doi>
  • Jenkins NE, Chakravorty SJ, Urban BC, Kai OK, Marsh K, Craig AG. 2006. The effect of Plasmodium falciparum infection on expression of monocyte surface molecules. Trans R Soc Trop Med Hyg, vol. 100(11), 1007-1012. link> doi>
  • Hollestelle MJ, Donkor C, Mantey EA, Chakravorty SJ, Craig A, Akoto AO, O'Donnell J, van Mourik JA, Bunn J. 2006. von Willebrand factor propeptide in malaria: evidence of acute endothelial cell activation. Br J Haematol, vol. 133(5), 562-569. link> doi>
  • CHAKRAVORTY SJ, Dormeyer M, Adams Y, Kramer B, Tsuey Tse M, Pegoraro S, Whittaker L, Lanzer M, Craig A. 2006. Rational Design of Anticytoadherence Inhibitors for Plasmodium falciparum Based on the Crystal Structure of Human Intercellular Adhesion Molecule 1. Antimicrobial Agents and Chemotherapy, vol. 50(2), 724-730. doi>
  • CHAKRAVORTY SJ, Buckley CD, Butler LM, McGettrick HM. 2006. An in vitro model for analysing neutrophil migration into and away from the sub-endothelial space: Roles of flow and CD31. Biorheology, vol. 43(1), 71-82.
  • Horrocks P, Pinches RA, Chakravorty SJ, Papakrivos J, Christodoulou Z, Kyes SA, Urban BC, Ferguson DJP, Newbold CI. 2005. PfEMP1 expression is reduced on the surface of knobless Plasmodium falciparum infected erythrocytes. J Cell Sci, vol. 118(Pt 11), 2507-2518. link> doi>
  • Chakravorty SJ and Craig A. 2005. The role of ICAM-1 in Plasmodium falciparum cytoadherence. Eur J Cell Biol, vol. 84(1), 15-27. link> doi>
  • Chakravorty SJ, Cockwell P, Girdlestone J, Brooks CJ, Savage COS. 2002. Fractalkine expression on human renal tubular epithelial cells: potential role in mononuclear cell adhesion. Clin Exp Immunol, vol. 129(1), 150-159. link> doi>
  • Cockwell P, Calderwood JW, Brooks CJ, Chakravorty SJ, Savage COS. 2002. Chemoattraction of T cells expressing CCR5, CXCR3 and CX3CR1 by proximal tubular epithelial cell chemokines. Nephrol Dial Transplant, vol. 17(5), 734-744. link> doi>
  • Cockwell P, Chakravorty SJ, Girdlestone J, Savage COS. 2002. Fractalkine expression in human renal inflammation. J Pathol, vol. 196(1), 85-90. link> doi>
  • Chakravorty SJ, Howie AJ, Girdlestone J, Gentle D, Savage CO. 2001. Potential role for monocyte chemotactic protein-4 (MCP-4) in monocyte/macrophage recruitment in acute renal inflammation. J Pathol, vol. 194(2), 239-246. link> doi>
  • Cockwell P, Brooks CJ, Girdlestone J, Chakravorty SJ, Adu D, Savage COS. 1999. Fractalkine mRNA expression in vivo and in vitro in human renal inflammation. KIDNEY INTERNATIONAL, vol. 55(6), 2584. link>
  • Chakravorty SJ, Howie AJ, Cockwell P, Adu D, Savage COS. 1999. T lymphocyte adhesion mechanisms within inflamed kidney. KIDNEY INTERNATIONAL, vol. 55(5), 2096. link>
  • Chakravorty SJ, Howie AJ, Cockwell P, Adu D, Savage CO. 1999. T lymphocyte adhesion mechanisms within inflamed human kidney: studies with a Stamper-Woodruff assay. Am J Pathol, vol. 154(2), 503-514. link> doi>
  • Chakravorty SJ, Howie AJ, Cockwell P, Adu D, Savage COS. 1997. T lymphocyte adhesion mechanisms within inflamed kidney. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, vol. 8, A2102. link>
  • Chakravorty SJ, Howie AJ, Cockwell P, Adu D, Savage COS, Wheeler D. 1996. T-cell adhesion processes in ANCA positive glomerulonephritis. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, vol. 7(9), A2236. link>

Other

  • Bridges DJ, Chakravorty SJ, Bunn J, O'Donnell J, Grau GE, de laat B, van Mourik JA, Craig A. 2008. The role of von Willebrand factor in malaria pathogenesis. INTERNATIONAL JOURNAL FOR PARASITOLOGY (vol. 38, p. S65). link>

Undergraduate modules:

  • LSC-30036: Human Parasitology: (Module Manager)
  • LSC-30028: Advances in Medicine: (Module Manager)
  • LSC-30015: Biology of Disease
  • Supervisor for Independent Study Final Year Projects
  • LSC-20056: Research and Analytical Skills
  • LSC-20052: Nutrition and Energy Balance
  • LSC-10031: Cell & Molecular Biology
  • LSC-10039:Human Physiology and Pathology
  • LSC-10040: Introduction to Human Physiology (Module Manager)
  • Supervisor to Placement student:  Institut Pasteur, Paris and Astra Zeneca, UK.


Postgraduate Taught Course:

  • Masters in Parasitology and Vector Biology