Dr Helen Price

Title: Lecturer in Bioscience
Phone: +44 (0)1782 734219
Email: h.price@keele.ac.uk
Location: Huxley Building : 171
Role: PGR lead for Life Sciences
Contacting me: Try my office or arrange an appointment by e-mail.
price helen

Trypanosome parasite I graduated from the University of Leeds with a BSc. (Hons.) degree in Applied Zoology.  I was awarded a PhD in 1997 following research at Bangor University on a vaccine candidate for the human parasite Schistosoma mansoni.  In my first research position, I studied novel genes involved in chromosome translocations in B-cell malignancies at the Institute of Cancer Research, Surrey.  I returned to the field of parasitology in 2000 to join the research group of Debbie Smith at Imperial College London and from 2004 at the University of York, studying the cell biology of the protozoan parasites Leishmania major and Trypanosoma brucei.  I performed molecular studies to validate the enzyme N-myristoyltransferase (NMT) as a drug target and used protozoa as genetically tractable model organisms to study the functions of proteins implicated in human genetic and autoimmune diseases.  I relocated to Keele as Lecturer in Bioscience in September 2013. 

Image: Scanning electron micrograph of Trypanosoma brucei bloodstream form parasite

Leishmania-infected macrophage My research focuses on understanding the cell biology of the protozoan parasites Leishmania and Trypanosoma brucei and exploiting this knowledge towards the development of new therapeutics.  I recently found that the Leishmania orthologue of the gene BBS1 (a subunit of the BBSome protein complex) is essential for survival of the parasite in the host.  I currently have projects on drug discovery (in collaboration with Prof Paul Horrocks from ISTM, Keele) characterisation of novel drug targets and the generation of transgenic Leishmania lines for use in drug screening.

I am also investigating the potential use of magnetic and gold hybrid nanoparticles as novel therapies for cutaneous leishmaniasis, in collaboration with Neil Telling and Clare Hoskins from the Institute for Science and Technology in Medicine (ISTM), Keele.

Image: Macrophage infected with Leishmania major amastigotes (Molecular Microbiology Vol. 90, November 2013)

Further details (CAEP Homepage)

Selected Publications

  • El-Faham MH, Wheatcroft-Francklow KJ, Price HP, Sayers JR, Doenhoff MJ. 2017. Schistosoma mansoni cercarial elastase (SmCE): differences in immunogenic properties of native and recombinant forms. Parasitology, vol. 144(10), 1356-1364. doi>
  • Sangaré I, Djibougou AD, Yaméogo BK, Drabo F, Diabaté A, Banuls A, Fournet F, Price HP, Guiguemdé RT, Dabiré RK. 2016. First Detection of Leishmania infantum in Domestic Dogs from Burkina Faso (West Africa). Research Journal of Parasitology. doi>
  • MacLean L, Price H, O'Toole P. 2016. Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell Imaging Methods. (vol. 1459). link> doi>
  • Wright MH, Paape D, Price HP, Smith DF, Tate EW. 2016. Global profiling and inhibition of protein lipidation in vector and host stages of the sleeping sickness parasite Trypanosoma brucei. ACS Infectious Diseases. doi>
  • MacLean L, Myburgh E, Rodgers J, Price HP. 2013. Imaging African trypanosomes. PARASITE IMMUNOLOGY, vol. 35(9-10), 283-294. link> doi>

Full Publications List show

Journal Articles

  • El-Faham MH, Wheatcroft-Francklow KJ, Price HP, Sayers JR, Doenhoff MJ. 2017. Schistosoma mansoni cercarial elastase (SmCE): differences in immunogenic properties of native and recombinant forms. Parasitology, vol. 144(10), 1356-1364. doi>
  • Sangaré I, Djibougou AD, Yaméogo BK, Drabo F, Diabaté A, Banuls A, Fournet F, Price HP, Guiguemdé RT, Dabiré RK. 2016. First Detection of Leishmania infantum in Domestic Dogs from Burkina Faso (West Africa). Research Journal of Parasitology. doi>
  • Wright MH, Paape D, Price HP, Smith DF, Tate EW. 2016. Global profiling and inhibition of protein lipidation in vector and host stages of the sleeping sickness parasite Trypanosoma brucei. ACS Infectious Diseases. doi>
  • MacLean L, Myburgh E, Rodgers J, Price HP. 2013. Imaging African trypanosomes. PARASITE IMMUNOLOGY, vol. 35(9-10), 283-294. link> doi>
  • Price H. 2013. Crystal structure of the small GTPase Arl6/BBS3 from Trypanosoma brucei. Protein Science, vol. 22(2), 196-203. doi>
  • Price H, Paape D, Hodgkinson MR, Farrant K, Stark M, Smith DF. 2013. The Leishmania major BBSome subunit BBS1 is essential for parasite virulence in the mammalian host (in press). Molecular Biology. doi>
  • Price H, Hodgkinson MR, Smith BA, Carrington M, Stark M, Smith DF. 2012. A role for the Vesicle-Associated Tubulin Binding Protein ARL6 (BBS3) in Flagellum Extension in Trypanosoma brucei. Biochimica et Biophysica Acta: Molecular Cell Research, vol. 1823(7), 1178-1191. doi>
  • Price H, Hodgkinson MR, Curwen RS, MacLean LM, Brannigan JA, Carrington M, Smith BA, Ashford DA, Stark M, Smith DF. 2012. The Orthologue of Sjogrens Syndrome Nuclear Autoantigen 1 (SSNA1) in Trypanosoma brucei is an Immunogenice self-assembling molecule. PLoS One, vol. 7, Article e31842. doi>
  • Ramos CS, Yokoyama-Yasunaka JK, Guerra-Giraldez C, Price H, Mortara RA, Smith DF, Uliana SR. 2011. Leishmania amazonensis META2 protein confers protection against heat shock and oxidative stress. Exp Parasitol, vol. 127, 228-237. doi>
  • Price H, Guther MLS, Ferguson MAJ, Smith DF. 2010. Myristoyl-CoA: protein N-myristoyltrasferase depletion in trypanosomes causes avirulence and endocytic defects. Molecular and Biochemical Parasitology, vol. 169(1), 55-58. doi>
  • Brannigan JA, Smith BA, Yu Z, Brzozowski AM, Hodgkinson MR, Maroof A, Price H, Meier F, Leatherbarrow RJ, Tate EW, Smith DF, Wilkinson AJ. 2010. N-myristoyltransferase from Leishmania donovani: structural and functional characterisation of a potential drug target for visceral leishmaniasis. Journal of Molecular Biology, vol. 396, 985-999. doi>
  • Frearson JA, Brand S, McElroy SP, Cleghorn LA, Smid O, Stojanovski L, Price H, Guther ML, Torrie LS, Robinson DA, Hallyburton I, Mpamhanga CP, Brannigan JA, Wilkinson AJ, Hodgkinson M, Hui R, Qiu W, Raimi OG, van Aalten DM, Brenk R, Gilbert IH, Read KD, Fairlamb AH, Ferguson MA, Smith DF, Wyatt PG. 2010. N-myristoyltransferase inhibitors as new leads to treat sleeping sickness. Nature, vol. 464, 728-732. doi>
  • Price H, Peltan A, Stark M, Smith DF. 2010. The small GTPase ARL2 is required for cytokinesis in Trypanosoma brucei. Molecular and Biochemical Parasitology, vol. 173, 123-131. doi>
  • Sadlova J, Price H, Smith BA, Votypka J, Volf P, Smith DF. 2010. The stage regulated HASPB and SHERP proteins are essential for differentiation of the protozoan parasite Leishmania major in its sand fly vector, Phlebotomus papatasi. Cell Microbiology, vol. 12, 1765-1779. doi>
  • Price H, MacLean L, Marrison J, O'Toole P, Smith DF. 2010. Validation of a new method for immobilising kinetoplastid parasites for live cell imaging. Molecular and Biochemical Parasitology, vol. 169(1), 66-69. doi>
  • Mills E, Price HP, Johner A, Emerson JE, Smith DF. 2007. Kinetoplastid PPEF phosphatases: dual acylated proteins expressed in the endomembrane system of Leishmania. Mol Biochem Parasitol, vol. 152(1), 22-34. link> doi>
  • Price HP, Stark M, Smith B, Smith DF. TbARF1 influences lysosomal function but not endocytosis in procyclic stage Trypanosoma brucei. Mol Biochem Parasitol, vol. 155, 123-127. link> doi>
  • Price HP, Stark M, Smith DF. Trypanosoma brucei ARF1 plays a central role in endocytosis and golgi-lysosome trafficking. Mol Biol Cell, vol. 18, 864-873. link> doi>
  • Panethymitaki C, Bowyer PW, Price HP, Leatherbarrow RJ, Brown KA, Smith DF. Characterization and selective inhibition of myristoyl-CoA:protein N-myristoyltransferase from Trypanosoma brucei and Leishmania major. Biochemical Journal, vol. 396(2), 277-285. link> doi> link>
  • Denny PW, Shams-Eldin H, Price HP, Smith DF, Schwarz RT. The protozoan inositol phosphorylceramide synthase: a novel drug target that defines a new class of sphingolipid synthase. J Biol Chem, vol. 281, 28200-28209. link> doi>
  • Price HP, Goulding D, Smith DF. ARL1 has an essential role in Trypanosoma brucei. Biochemical Society Transactions, vol. 33(4), 643-645. link> doi> link>
  • Price HP, Panethymitaki C, Goulding D, Smith DF. Functional analysis of TbARL1, an N-myristoylated Golgi protein essential for viability in bloodstream trypanosomes. Journal of Cell Science, vol. 118, 831-841. link> doi> link>
  • Kropf P, Freudenberg MA, Modolell M, Price HP, Herath S, Antoniazi S, Galanos C, Smith DF, Muller I. 2004. Toll-like receptor 4 contributes to efficient control of infection with the protozoan parasite Leishmania major. Infection and Immunity, vol. 72(4), 1920-1928. link> doi> link>
  • Antoniazi S, Price HP, Kropf P, Freudenberg MA, Galanos C, Smith DF, Muller I. Chemokine gene expression in toll-like receptor-competent and -deficient mice infected with Leishmania major. Infect Immun, vol. 72, 5168-5174. link> doi>
  • Price HP, Menon MR, Panethymitaki C, Goulding D, McKean PG, Smith DF. Myristoyl-CoA:protein N-myristoyltransferase, an essential enzyme and potential drug target in kinetoplastid parasites. J Biol Chem, vol. 278, 7206-7214. link> doi>
  • Satterwhite E, Sonoki T, Willis TG, Harder L, Nowak R, Arriola EL, Liu H, Price HP, Gesk S, Steinemann D, Schlegelberger B, Oscier DG, Siebert R, Tucker PW, Dyer MJ. The BCL11 gene family: involvement of BCL11A in lymphoid malignancies. Blood, vol. 98(12), 3413-3420. link> doi> link>
  • Sanchez-Izquierdo D, Siebert R, Harder L, Marugan I, Gozzetti A, Price HP, Gesk S, Hernandez-Rivas JM, Benet I, Sole F, Sonoki T, Le Beau MM, Schlegelberger B, Dyer MJ, Garcia-Conde J, Martinez-Climent JA. Detection of translocations affecting the BCL6 locus in B cell non-Hodgkin's lymphoma by interphase fluorescence in situ hybridization. Leukemia, vol. 15(9), 1475-1484. link> link>
  • Willis TG, Jadayel DM, Du MQ, Peng H, Perry AR, Abdul-Rauf M, Price H, Karran L, Majekodunmi O, Wlodarska I, Pan L, Crook T, Hamoudi R, Isaacson PG, Dyer MJ. 1999. Bcl10 is involved in t(1;14)(p22;q32) of MALT B cell lymphoma and mutated in multiple tumor types. Cell, vol. 96(1), 35-45. link> doi> link>
  • Darani HY, Curtis RH, McNeice C, Price HP, Sayers JR, Doenhoff MJ. Schistosoma mansoni: anomalous immunogenic properties of a 27 kDa larval serine protease associated with protective immunity. Parasitology, vol. 115(3), 237-247. link>
  • Price HP, Doenhoff MJ, Sayers JR. Cloning, heterologous expression and antigenicity of a schistosome cercarial protease. Parasitology, vol. 114(5), 447-453. link>
  • Sayers JR, Price HP, Fallon PG, Doenhoff MJ. AGA/AGG codon usage in parasites: implications for gene expression in Escherichia coli. Parasitol Today, vol. 11(9), 345-346. link> doi> link>

Chapters

  • MacLean L, Price H, O'Toole P. 2016. Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell Imaging Methods. (vol. 1459). link> doi>

Year 1

  • LSC-10036 Cells and Organelles: Biochemical Aspects of Cell Biology
  • LSC-10038 Information and Inheritance
  • LSC-10032 Genetics and Evolution

Year 2

  • LSC-20001 Animal Adaptations
  • LSC-20003 Gene and Protein Engineering
  • LSC-20050 Human Genetics
  • LSC-20056 Research and Analytical Skills
  • LSC-20073 Microbes, Viruses and Parasites (Module lead, beginning October 2017)

Year 3

  • LSC-30009 Clinical Pathology
  • LSC-30015 Biology of Disease
  • LSC-30035 Double Biochemistry Research Project 

Postgraduate

  • MSc. Molecular Parasitology and Vector Biology (University of Salford and Keele)
  • MSc. Biomedical Blood Science
  • MSc. Scientific Research Training with International Placement
Grant Funding
  • Wellcome Trust Seed Award (Oct 2015 – Mar 2017). Title: “Nanoparticle-induced hyperthermia as a novel therapy for cutaneous leishmaniasis”. Total awarded: £92,606.00
  • Royal Society research grant (Mar 2014 - Mar 2015). Title: “Functions and interactions of BBS1, a novel virulence factor in the human pathogen Leishmania.” Total awarded: £14,159.32

Management and Administrative Roles
  • Postgraduate Research Lead for School of Life Sciences
  • Vice-Chair of the Keele University Ethical Review Panel 3 (ERP3)
  • Year Lead for 2nd year Biochemistry
  • Elected member of Keele University Senate
  • Academic representative and Pathogen Advisor for the Keele Genetic Modification Safety Sub-committee (GMSSC)
  • Ordinary Member of Council for the British Society for Parasitology