Dr David Watson

Title: Lecturer in Biochemistry
Phone: +44 (0)1782 733676
Email: d.watson@keele.ac.uk
Location: 303b
Role: Academic Conduct Officer (ACO), first year tutor (Biochemistry and Biomedical Science), Biochemistry laboratory coordinator, personal tutor
Contacting me: Try my office or arrange an appointment by email.
david watson

I was appointed as a Teaching Fellow in Biochemistry in the School of Life Sciences in 2010 and then to Lecturer in 2013. I graduated with a BSc (Hons) in Biochemistry with Biological Medicinal Chemistry from Keele in 2005 and returned in November of the same year to begin a PhD under the supervision of Dr Anne Loweth investigating mechanisms of pancreatic beta cell destruction induced by inflammatory cytokines and by reactive oxygen and nitrogen species in the pathogenesis of type 1 diabetes mellitus, which I completed in 2009. During this time I worked as a GTA in the School of Medicine and as a demonstrator in the School of Life Sciences, largely within the year 1 & 2 Biochemistry and Biomedical Sciences degree course before subsequently being appointed to a permanent academic position.

My main research interests are in biochemical cell biology and cell signalling, focussing on the regulation of programmed cell death, apoptosis. My PhD research aimed at investigating some of the mechanisms contributing to pancreatic beta cell death and apoptosis in response to inflammatory cytokines and reactive nitrogen and oxygen species. This work focussed on the role of intracellular calcium movements and the activation of downstream endoplasmic reticulum stress signalling events. Targeted beta cell destruction precedes the development of long term complications including diabetic nephropathy, neuropathy and retinopathy, brought about in part by elevated levels of reactive oxygen intermediates, plasma free fatty acids and high peripheral glucose concentrations, together this may indicate common mechanisms of cellular dysfunction in diabetes and its associated complications.    I also have research links with Professor Gwyn Williams and Dr Mirna Mourtada-Maarabouni where investigations focus on defining the expression and function of novel apoptosis controlling genes. 

Current projects include:

  • The role of eicosanoids in beta cell dysfunction using in vitro models of type 1 and 2 DM
  • The functional role(s) of long non-coding RNAs in beta cell dysfunction
  • The cytoprotective effects of novel plant extracts against pro-inflammatory cytokine-mediated toxicity and in response to glucolipotoxicity in beta cell lines

Selected Publications

  • Grimes D and Watson D. 2017. Structure: activity relationship for the cytoprotective effects of epoxyeicosatrienoic acids (EETs) and their corresponding diols against palmitate-induced lipotoxicity in BRIN-BD11 cells. DIABETIC MEDICINE (vol. 34, p. 43). link> doi>
  • Cunningham R, Barringer R, Hussain R, Watson D. 2015. A role for the long non-coding RNA growth arrest specific transcript 5 (GAS5) in cytokine-induced beta cell death. DIABETIC MEDICINE (vol. 32, p. 39). link>
  • Mourtada-Maarabouni M, Watson D, Munir M, Farzaneh F, Williams GT. 2013. Apoptosis suppression by candidate oncogene PLAC8 is reversed in other cell types. Curr Cancer Drug Targets, vol. 13(1), 80-91. link> doi>
  • Watson D and Loweth AC. 2009. Oxidative and nitrosative stress in beta-cell apoptosis: their contribution to beta-cell loss in type 1 diabetes mellitus. Br J Biomed Sci, vol. 66(4), 208-215. link> doi>
  • Watson D, Kinsey E, Hazelwood S, Scarpello J, Loweth A. 2007. Differential use of calcium stores in reactive oxygen species-dependent pancreatic beta cell death. DIABETOLOGIA (vol. 50, p. S182). link>

Full Publications List show

Journal Articles

  • Mourtada-Maarabouni M, Watson D, Munir M, Farzaneh F, Williams GT. 2013. Apoptosis suppression by candidate oncogene PLAC8 is reversed in other cell types. Curr Cancer Drug Targets, vol. 13(1), 80-91. link> doi>
  • Watson D and Loweth AC. 2009. Oxidative and nitrosative stress in beta-cell apoptosis: their contribution to beta-cell loss in type 1 diabetes mellitus. Br J Biomed Sci, vol. 66(4), 208-215. link> doi>
  • Grimes D and Watson D. FORTHCOMING: Epoxyeicosatrienoic acids but not their corresponding diols protect against palmitate-induced lipotoxicity in BRIN-BD11 cells.

Other

  • Grimes D and Watson D. 2017. Structure: activity relationship for the cytoprotective effects of epoxyeicosatrienoic acids (EETs) and their corresponding diols against palmitate-induced lipotoxicity in BRIN-BD11 cells. DIABETIC MEDICINE (vol. 34, p. 43). link> doi>
  • Cunningham R, Barringer R, Hussain R, Watson D. 2015. A role for the long non-coding RNA growth arrest specific transcript 5 (GAS5) in cytokine-induced beta cell death. DIABETIC MEDICINE (vol. 32, p. 39). link>
  • Watson D, Kinsey E, Hazelwood S, Scarpello J, Loweth A. 2007. Differential use of calcium stores in reactive oxygen species-dependent pancreatic beta cell death. DIABETOLOGIA (vol. 50, p. S182). link>
  • Watson D, Kinsey E, Hoskert BS, Hazlewood SA, Scarpello JHB, Loweth AC. 2007. A role for mitochondrial calcium uptake in oxidant-induced beta-cell death. DIABETIC MEDICINE (vol. 24, p. 34). link>
  • LSC 10034 Natures Tools: Proteins & Enzymes (Module Manager)
  • LSC 10036 Cells and Organelles: Biochemical Aspects of Cell Biology
  • LSC 20003 Gene and Protein Engineering
  • LSC 20015 Molecular, Cellular and Structural immunology
  • LSC 20016 Metabolism in Health and Disease (Module Manager)
  • LSC-20052 Nutrition and Energy Balance
  • LSC 30008/35 Research Project (Experimental)
  • LSC 30010 Acquisition, Analysis and Communication of Information (Module Manager)
  • LSC 30014 Research Project (Non-Experimental)
  • LSC 30015 Biology of Disease
  • LSC-30028 Advances in Medicine
  • LSC-40033 Advanced Laboratory Techniques
  • LSC-40040 Dissertation: Blood Sciences Research Project
Current lab members:

Daniel Grimes

Ali Shirian