Dr Dan Tonge

Title: Lecturer in Biosciences
Phone: +44 (0)1782 733418
Email: d.p.tonge@keele.ac.uk
Location: Huxley Building : 170
Role:
Contacting me: Try my office or arrange an appointment by e-mail.
dan tonge

Dr Daniel Tonge graduated with a BSc(Hons) in Biomedical Sciences incorporating a year-long clinical training placement in histopathology and cytology at the Royal Liverpool and Broadgreen Hospital. Following graduation, he completed a PhD in Molecular Biology at the University of Nottingham with funding from AstraZeneca and Arthritis Research UK. His doctoral thesis investigated molecular changes in the quadriceps skeletal muscle in various experimental models of knee osteoarthritis. After completing his doctoral studies, Dr Tonge undertook post-doctoral research positions at the School of Veterinary Medicine, Nottingham (“molecular control of skeletal muscle fibre type”), the Medical Research Council (“next generation sequencing of small non-coding RNAs for biomarker discovery”) and the Health Protection Agency, Oxford. In 2012, he was appointed as a Senior Scientist at the Health Protection Agency (Public Health England thereafter) with responsibility for a range of research projects covering nucleic acid biomarker discovery and next generation sequencing-driven fungal metagenomics. Whilst at PHE, Dr Tonge was responsible for the implementation and management of a local next generation sequencing facility and was a member of the agency-wide next generation sequencing and bioinformatics working group. In May 2015, Dr Tonge joined Keele University as a Lecturer in Biosciences.

 

Dr Tonge's current research involves the application (and development) of next generation sequencing and bioinformatic techniques to the areas of personalised medicine (key disease pathway analysis and novel biomarker discovery) and microbial metagenomics.
Current projects include;

  • An analysis of the circulating miRNA profile of severe, poorly controlled asthmatic patients
  • The role of obesity in the pathogenesis of hip osteoarthritis – elucidation of key nucleic acid signatures
  • An analysis of circulating microbial nucleic acids in hip osteoarthritis patients
  • Metagenomic analyses of the rhizosphere microbiome and its impact on VOC detoxification (PhD Studentship)

Although I work in all disease areas, I have a particular interest in the pathogenesis of Osteoarthritis and the potential for the development of personalised / precision therapeutics.

Selected Publications

  • Tonge DP and Gant TW. 2016. What is normal? Next generation sequencing-driven analysis of the human circulating miRNAOme. BMC Mol Biol, vol. 17, 4. link> doi> full text>
  • Tonge DP. 2014. Global miRNA profiling for biomarker discovery – What is normal?. Toxicology Letters. Elsevier. doi>
  • Tonge DP, Pashley CH, Gant TW. Amplicon -based Metagenomic Analysis of Mixed Fungal Samples using Proton Release Amplicon Sequencing. PLoS One.
  • Tonge DP and Jones SW. 2014. The hallmarks of osteoarthritis and the potential to develop personalised disease-modifying pharmacological therapeutics. Osteoarthritis and Cartilage, vol. 22(5), 609-621. doi> link>
  • Tonge DP, Parr T, Bardsley R, Jones SW. Evidence of Changes to Skeletal Muscle Contractile Properties During the Initiation of Disease in the Ageing Guinea Pig Model of Osteoarthritis. Longevity and Healthspan. doi>

Full Publications List show

Journal Articles

  • Tonge DP and Gant TW. 2016. What is normal? Next generation sequencing-driven analysis of the human circulating miRNAOme. BMC Mol Biol, vol. 17, 4. link> doi> full text>
  • Tonge DP, Pashley CH, Gant TW. Amplicon -based Metagenomic Analysis of Mixed Fungal Samples using Proton Release Amplicon Sequencing. PLoS One.
  • Tonge DP and Jones SW. 2014. The hallmarks of osteoarthritis and the potential to develop personalised disease-modifying pharmacological therapeutics. Osteoarthritis and Cartilage, vol. 22(5), 609-621. doi> link>
  • Tonge DP, Parr T, Bardsley R, Jones SW. Evidence of Changes to Skeletal Muscle Contractile Properties During the Initiation of Disease in the Ageing Guinea Pig Model of Osteoarthritis. Longevity and Healthspan. doi>
  • Tonge DP, Jones SW, Bardsley RG, Parr T. Characterisation of the sarcomeric myosin heavy chain multigene family in the laboratory guinea pig. BMC Molecular Biology. doi>
  • Tonge DP and Gant TW. Evidence Based Housekeeping Gene Selection for microRNA-sequencing (miRNA-seq) Studies. Toxicological Research. doi>
  • Tonge DP, Kelsall J, Tugwood J, Gant TW. The role of microRNAs in the pathogenesis of MMPi-induced skin fibrodysplasia. BMC Genomics. doi>
  • Tonge DP, Bardsley R, Jones SW, Doherty M, Maciewicz RA, Parr T. β2-Adrenergic agonist-induced hypertrophy of the quadriceps skeletal muscle does not modulate disease severity in the rodent meniscectomy model of osteoarthritis. Osteoarthritis and Cartilage. doi>

Other

  • Tonge DP. 2014. Global miRNA profiling for biomarker discovery – What is normal?. Toxicology Letters. Elsevier. doi>
  • Tonge DP. 2012. The application of Next Generation Sequencing to the detection and analysis of miRNAs. Toxicology Letters. Elsevier. doi>