Dr Nikki Kuiper

Title: Senior Lecturer in Biomedical Sciences
Phone: +44 (0)1691404451 (Oswestry) or +44 (0)1782 734653 (Keele)
Email: n.j.kuiper@keele.ac.uk
Location: Institute for Science & Technology in Medicine, Keele University,The Arthritis Research Centre, The Robert Jones & Agnes Hunt Orthopaedic Hospital, Oswestry, Shropshire, SY11 2RU.
Role: ISTM Research theme: Regenerative Medicine
Contacting me: Try my office or arrange an appointment by e-mail.
Dr Nikki Kuiper

Year (date)

Position

Institution and location

2008-

 

Senior Lecturer 

 

 Medical School, Institute of Science & Technology in  Medicine, University of Keele.

2003-2008

 

Lecturer

 

 Medical School, Institute of Science & Technology in  Medicine, University of Keele.

1999-2003

 

PDRA

 

 Arthritis Research Centre, Robert Jones & Agnes Hunt  Orthopaedic Hospital, Oswestry, Shropshire.

1997-1999

 

PDRA

 

 The Wellcome Trust Centre for Cell-Matrix Research,  Medical School, University of Manchester, Manchester.

1995-1997

PDRA

 

 The Lerner Research Institute, The Cleveland Clinic  Foundation, USA.

ISTM Research theme: Regenerative Medicine

Nikki joined the new Medical School at The University of Keele in 2003 and rapidly built up a fully equipped laboratory benefitting from investment from the EPSRC, ARUK, Charity and Industry. She has an internationally recognised profile for research into the biology and pathology of chondrocytes, chondrons and cartilage with expertise in the analysis of cartilage (Wang et al. Eur Cell Mater. 2010; Wang et al. J Anat. 2008). She has established a sensitive and discriminatory approach to glycan analysis in very small tissue extracts using fluorophore-assisted carbohydrate electrophoresis (FACE; Sharma et al. The Knee 2017; Kuiper et al. Osteoarthritis Cartilage 2015; Sharma et al. Arthritis Res Ther. 2007). With EPSRC funding, she studied the influence of mechanical loading on single chondrocytes, encapsulated chondrocytes and chondrons using micromanipulation (Wang et al. Biomaterials 2010; Nguyen et al. J R Soc Interface. 2010; Nguyen et al. Biotechnol Lett 2009). This work led to the development of a flow perfusion bioreactor for 3D osteochondral tissue constructs (Kuiper et al. J Biomater Tissue Eng. 2014; Whittaker et al. J Theor Biol. 2009).

Nikki was promoted to Senior Lecturer in 2008.

In 2010 she took a 4-year research career break to raise family returning in 2015 to take up an ISTM fellowship. Her research base is at the RJAH Orthopaedic Hospital at Oswestry which is a partner to Keele. NJK has served on the British Society for Matrix Biology committee and is a member of the EPSRC peer review college and the ARUK college of experts.

 

Selected Publications

  • Owida HA, De Las Heras Ruiz T, Dhillon A, Yang Y, Kuiper NJ. 2017. Co-culture of chondrons and mesenchymal stromal cells reduces the loss of collagen VI and improves extracellular matrix production. Histochem Cell Biol, vol. 148(6), 625-638. link> doi>
  • Sharma A, Rees D, Roberts S, Kuiper NJ. 2017. A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improves as the tissue matures. The Knee, vol. 24(1), 149-157. doi> link>
  • Kuiper NJ and Sharma A. 2015. A detailed quantitative outcome measure of glycosaminoglycans in human articular cartilage for cell therapy and tissue engineering strategies. Osteoarthritis Cartilage, vol. 23(12), 2233-2241. link> doi>
  • Dale TP, de Castro A, Kuiper NJ, Parkinson EK, Forsyth NR. 2015. Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner. PLoS One, vol. 10(7), e0133745. link> doi>
  • Dale TP, De Castro A, Parkinson EK, Kuiper NJ, Forsyth NR. 2014. Immortalisation with hTERT impacts on sulphated glycosaminoglycan secretion and chondrogenic potential in a variable and cell specific manner. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, vol. 8, 366. link>

Full Publications List show

Journal Articles

  • Owida HA, De Las Heras Ruiz T, Dhillon A, Yang Y, Kuiper NJ. 2017. Co-culture of chondrons and mesenchymal stromal cells reduces the loss of collagen VI and improves extracellular matrix production. Histochem Cell Biol, vol. 148(6), 625-638. link> doi>
  • Sharma A, Rees D, Roberts S, Kuiper NJ. 2017. A case study: Glycosaminoglycan profiles of autologous chondrocyte implantation (ACI) tissue improves as the tissue matures. The Knee, vol. 24(1), 149-157. doi> link>
  • Kuiper NJ and Sharma A. 2015. A detailed quantitative outcome measure of glycosaminoglycans in human articular cartilage for cell therapy and tissue engineering strategies. Osteoarthritis Cartilage, vol. 23(12), 2233-2241. link> doi>
  • Dale TP, de Castro A, Kuiper NJ, Parkinson EK, Forsyth NR. 2015. Immortalisation with hTERT Impacts on Sulphated Glycosaminoglycan Secretion and Immunophenotype in a Variable and Cell Specific Manner. PLoS One, vol. 10(7), e0133745. link> doi>
  • Dale TP, De Castro A, Parkinson EK, Kuiper NJ, Forsyth NR. 2014. Immortalisation with hTERT impacts on sulphated glycosaminoglycan secretion and chondrogenic potential in a variable and cell specific manner. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, vol. 8, 366. link>
  • Kuiper NJ, Wang QG, Cartmell SH. 2014. A Perfusion Co-Culture Bioreactor for Osteochondral Tissue Engineered Plugs. JOURNAL OF BIOMATERIALS AND TISSUE ENGINEERING, vol. 4(2), 162-171. link> doi>
  • Clarkin CE, Allen S, Kuiper NJ, Wheeler BT, Wheeler-Jones CP, Pitsillides AA. 2011. Regulation of UDP-glucose dehydrogenase is sufficient to modulate hyaluronan production and release, control sulfated GAG synthesis, and promote chondrogenesis. J Cell Physiol, vol. 226(3), 749-761. link> doi>
  • Nguyen BV, Wang QG, Kuiper NJ, El Haj AJ, Thomas CR, Zhang Z. 2010. Biomechanical properties of single chondrocytes and chondrons determined by micromanipulation and finite-element modelling. J R Soc Interface, vol. 7(53), 1723-1733. link> doi>
  • Wang QG, Hughes N, Cartmell SH, Kuiper NJ. 2010. The composition of hydrogels for cartilage tissue engineering can influence glycosaminoglycan profile. Eur Cell Mater, vol. 19, 86-95. link> doi>
  • Wang QG, Nguyen B, Thomas CR, Zhang Z, El Haj AJ, Kuiper NJ. 2010. Molecular profiling of single cells in response to mechanical force: comparison of chondrocytes, chondrons and encapsulated chondrocytes. Biomaterials, vol. 31(7), 1619-1625. link> doi>
  • Nguyen BV, Wang Q, Kuiper NJ, El Haj AJ, Thomas CR, Zhang Z. 2009. Strain-dependent viscoelastic behaviour and rupture force of single chondrocytes and chondrons under compression. Biotechnol Lett, vol. 31(6), 803-809. link> doi>
  • Whittaker RJ, Booth R, Dyson R, Bailey C, Parsons Chini L, Naire S, Payvandi S, Rong Z, Woollard H, Cummings LJ, Waters SL, Mawasse L, Chaudhuri JB, Ellis MJ, Michael V, Kuiper NJ, Cartmell S. 2009. Mathematical modelling of fibre-enhanced perfusion inside a tissue-engineering bioreactor. J Theor Biol, vol. 256(4), 533-546. link> doi>
  • Wang QG, Magnay JL, Nguyen B, Thomas CR, Zhang Z, El Haj AJ, Kuiper NJ. 2009. Gene expression profiles of dynamically compressed single chondrocytes and chondrons. Biochem Biophys Res Commun, vol. 379(3), 738-742. link> doi>
  • Wang QG, El Haj AJ, Kuiper NJ. 2008. Glycosaminoglycans in the pericellular matrix of chondrons and chondrocytes. J Anat, vol. 213(3), 266-273. link> doi>
  • Sharma A, Wood LD, Richardson JB, Roberts S, Kuiper NJ. 2007. Glycosaminoglycan profiles of repair tissue formed following autologous chondrocyte implantation differ from control cartilage. Arthritis Res Ther, vol. 9(4), R79. link> doi>
  • KUIPER NJ, Ashton B, Cartwright A. 2004. Effects of High Molecular Weight Hyaluronan on Chondrocytes Cultured within a Resorbable Gelatin Sponge. Tissue Engineering, vol. 10(3-4), 621-631. doi>
  • Bailey A, GOODSTONE NJ, Roberts S, Hughes J, ROBERTS S, van NIEKERK L, RICHARDSON J, REES D. 2003. Rehabilitation After Oswestry Autologous-Chondrocyte Implantation: The OsCell Protocol. Journal of Sports Rehabilitation, vol. 12(2), 104-118.
  • Goodstone NJ and Hardingham TE. 2002. Tumour necrosis factor alpha stimulates nitric oxide production more potently than interleukin-1beta in porcine articular chondrocytes. Rheumatology (Oxford), vol. 41(8), 883-891. link> doi>
  • KUIPER NJ and Hardingham TE. 2002. Tumour necrosis factor alpha stimulates nitric oxide production more potently than interleukin-1beta in porcine articular chondrocytes. Rheumatology, vol. 41(8), 1-9. doi>
  • Goodstone NJ, Hascall VC, Calabro A. 1998. Differential effects of Staphylococcus aureus alpha-hemolysin on the synthesis of hyaluronan and chondroitin sulfate by rat chondrosarcoma chondrocytes. Arch Biochem Biophys, vol. 350(1), 26-35. link> doi>
  • Goodstone NJ, Doran MC, Hobbs RN, Butler RC, Dixey JJ, Ashton BA. 1996. Cellular immunity to cartilage aggrecan core protein in patients with rheumatoid arthritis and non-arthritic controls. Ann Rheum Dis, vol. 55(1), 40-46. link> doi>
  • Doran MC, Goodstone NJ, Hobbs RN, Ashton BA. 1995. Cellular immunity to cartilage link protein in patients with inflammatory arthritis and non-arthritic controls. Ann Rheum Dis, vol. 54(6), 466-470. link> doi>
  • HOBBS RN, GOODSTONE NJ, DORAN M, JONES CE, MEE JB, ASHTON BA. 1992. AUTOIMMUNE RESPONSES TO ARTICULAR-CARTILAGE PROTEOGLYCAN CORE PROTEIN IN PATIENTS WITH INFLAMMATORY AND NONINFLAMMATORY ARTHRITIS. BRITISH JOURNAL OF RHEUMATOLOGY, vol. 31, 44. link>
  • HOBBS RN, GOODSTONE NJ, DORAN M, ASHTON BA. 1992. CELLULAR-IMMUNITY TO CARTILAGE LINK PROTEIN, HSP65 AND THE ARTHRITOGENIC NONAPEPTIDE IN PATIENTS WITH INFLAMMATORY AND NONINFLAMMATORY ARTHRITIS. BRITISH JOURNAL OF RHEUMATOLOGY, vol. 31, 44. link>
  • Goodstone NJ, Hobbs RN, Ashton BA. 1990. Responses of T-cells from patients with inflammatory arthritis to human cartilage antigens. Biochem Soc Trans, vol. 18(5), 955. link> doi>

Chapters

  • Calabro A, Midura R, Hascall V, Plaas A, Goodstone NJ, Roden L. 2000. Biosynthesis of chondroitin sulfate and hyaluronan. In Proteoglycans: Structure, Biology and Molecular Interactions. Iozzo RV (Ed.). New York, USA: Marcel Dekker Inc..
  • Hascall V, Fulop C, Salustri A, Goodstone NJ, Calabro A, Hogg M, Tammi R, Tammi M, Maccallum D. 1998. Metabolism of hyaluronan. In The chemistry, biology and medical applications of hyaluronan and its derivatives. Laurent TC (Ed.). (vol. 72). Portland Press.

Other

  • Hughes N, Cartmell SH, Kuiper NJ. 2009. Comparison of hydrogels for the optimal design of an osteochondral construct. International Journal of Experimental Pathology: Wiley-Blackwell Publishing.
  • Nguyen B, Zhang Z, Thomas C, Wang QG, Kuiper NJ, El Haj AJ. 2009. Mechanical properties of single chondrocytes and chondrons determined by microcompression technique and numerical modelling. Transactions (vol. 34, p. 323).
  • Wang QG, El Haj AJ, Kuiper NJ. 2008. Characterisation of the glycosaminoglycans in the pericellular matrix of chondrons and chondrocytes following enzymatic isolation. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 89, pp. A6-A7). link>
  • Wood LD, El Haj A, Harrison A, Kuiper NJ. 2008. Low intensity pulsed ultrasound (LIPUS) increases extracellular matrix synthesis by chondrocytes. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 89, pp. A17-A18). link>
  • Sharma A, Wood LD, Richardson JB, Roberts S, Kuiper NJ. 2008. The glycosaminoglycan profiles of repair tissue formed following autologous chondrocyte implantation differs from control cartilage. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY (vol. 89, p. A23). link>
  • Kuiper NJ, Gabriel N, Griffiths S, Tew S. 2008. BSMB Autumn 2007 Meeting Report Shaping and Sensing the Extracellular Matrix. International Journal of Experimental Pathology: Wiley InterScience.
  • CARTMELL H, GITTINGS J, TURNER I, CHAUDURI J, ELLIS M, WATERS S, CUMMINGS L, KUIPER NJ, MICHAEL V. 2007. Bioreactor Design for Osteochondral Tissue. Journal of Bone and Mineral Research (vol. 22, p. S162).
  • MICHAEL V, GITTINGS J, TURNER I, CHAUDURI J, ELLIS M, WATERS S, CUMMINGS L, GOODSTONE NJ, CARTMELL S. 2007. Co-culture bioreactor design for skeletal tissue engineering.
  • SHARMA A, RICHARDSON J, ROBERTS S, GOODSTONE N. 2006. Detailed analysis of glycosaminoglycans in an age-range of human cartilage biopsies. Transactions.
  • SHARMA A, RICHARDSON J, ROBERTS S, GOODSTONE N. 2006. Detailed analysis of glycosaminoglycans in human cartilage biopsies. Transactions (vol. 31, p. 1476).
  • GOODSTONE N, GARGIULO B, CARTWRIGHT A, ASHTON B. 2003. Effects of high molecular weight hyaluronan on chondrocytes cultured within Spongostan, a resorbable gelatin sponge. Transactions (vol. 20, p. 969).
  • Goodstone N, Gargiulo B, Cartwright A, Ashton B. 2003. Effects of high molecular weight hyaluronan on chondrocytes cultured within Spongostan, aresorbable gelatin sponge. 49th Annual Meeting, Orthopaedic Research Society.
  • Wang QG, Magnay J, Nguyen B, Thomas CR, Zhang Z, El Haj AJ, Kuiper NJ. 2009. The Relationship between Mechanical Forces and Gene Expression of Single Chondrocytes and Chondrons. Transactions (vol. 34, p. 297).

Nikki joined the medical school academic team in 2003 and helped to establish the pre-clinical medical programme at Keele as well as delivering teaching within the School of Life Sciences and the School of Health & Rehabilitation. Nikki has held a number of academic roles including year 1 & 2 OSSE lead, year 2 Movement & Trauma unit lead, and year 1 Medicine MBChB co-lead.  

Currently Nikki is the lead for year 1 & 2 written assessments and she delivers musculoskeletal teaching across years 1 and 2. 

Projects and Funding

 

Date

Funding (£)

Investigators

Source

Project Title

2016-2018

13,000

NJ Kuiper

Orthopaedic Institute, Oswestry

Cell therapies for chondral and osteochondral defects in the foot and ankle.

2016-2017

85,000

O Kehoe (PI), NJ Kuiper, NR Forsyth, P Roach

Orthopaedic Institute, Oswestry

Extracellular vesicles derived from mesenchymal stem cells and chondrocytes: characterisation and potential applications.

2016-2019

590,000

JH Kuiper (PI), O Kehoe, NJ Kuiper, C Stewart, S Roberts, JB Richardson, K Wright, J Oxtby, B Tins

MRC

How do cartilage injuries heal naturally? An experimental study in humans.

2015-2018

59,850 

NJ Kuiper (PI), A Bing, JB Richardson, S Roberts, JH Kuiper, N Makwana, P Laing

Orthopaedic Institute, Oswestry

Cell therapies for chondral and osteochondral defects in the foot and ankle.

 

2015-2016

28,500

NJ Kuiper (PI), NR Forsyth

Orthopaedic Institute, Oswestry

Glycosaminoglycan production by human embryonic stem cells, mesenchymal stem cells and adult articular chondrocytes.

2009-

484,207

S Roberts (PI), JB Richardson, NJ Kuiper, I McCall, E Johnson, JH Kuiper

ARUK

Developing and evaluating the use of cell implantation therapy for the repair of cartilage in OA.

2005-2010

220,000

NJ Kuiper & S Cartmell

Ruth Kent Memorial Fund

Relief of osteoarthritis using tissue engineering strategies.

2005-2008

282,912

NJ Kuiper, A El Haj, Z Zhang, C Thomas

EPSRC

Micromechanical analysis of chondrocytes; role of the pericellular matrix.

2004-2007

30,000

NJ Kuiper

Smith & Nephew Plc

Analysis of the effects of low intensity ultrasound on chondrocytes.

2004-2006

92,323

NJ Kuiper (PI), JB Richardson

ARUK

A novel approach for characterising repair cartilage following human ACI. 

 

Esteem Indicators

External Examiner: University of Manchester Medical School: Tissue Engineering for Regenerative Medicine (MRes, PGDip, PGCert) 2014-2017

Stakeholder governor: Council of Governors at the RJAH Orthopaedic Hospital, NHS Foundation Trust 2011-2013

Committee role: Elected to the British Society for Matrix Biology (BSMB) committee 2006-2009 (organised a BSMB Autumn conference ‘shaping & sensing the extracellular matrix’ for 100+ people at Keele University)

Society membership: British Society for Matrix Biology (BSMB), Orthopaedic Research Society (ORS), Tissue and Cellular Engineering Society (TCES)

 

Graduate Students

Current

Jess Sykes PhD (Lead Supervisor) - Cell therapies for osteochondral defects in the foot and ankle.

Amy Byrne PhD (Supervisor) - How do cartilage injuries heal naturally? An experimental study in humans.

Tim Hopkins PhD (Supervisor) - Investigation of the impact of bone health & activity levels on cartilage repair.

Hamza Abu Owida PhD (Supervisor) - Exploration of anisotropic templates and new cells sources for articular cartilage regeneration.

 

Former

Tina Dale PhD (Supervisor) 2014 - An exploration of cell suitability for articular cartilage repair and regeneration therapies.

Lindsay Wood MPhil (Lead Supervisor) 2010 - Effects of low intensity pulsed ultrasound on chondrocytes.

Qi Guang Wang PhD (Lead Supervisor) 2009 - Single-cell mechanics in cartilage tissue engineering