Dr Chris Duff

Title: Pre-registration Clinical Biochemist
Phone: +44 (0)1782 674264
Email: chris.duff@uhns.nhs.uk
Location: Department of Clinical Biochemistry, University Hospital of North Staffordshire, Stoke-on-Trent, ST4 6QG
Role: ISTM research theme:
Contacting me: By e-mail or phone please.
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Following the completion of my BSc at the University of Leeds, I went on to study for a PhD, working on a project investigating the post-translational regulation of the LDL receptor (LDLR) and its role in dyslipidaemia.  Central to this were cell biological and biophysical studies of proprotein convertase subtilisin/kexin type 9 (PCSK9), a serine protease that modulates the LDLR receptor.  It is the third genetic locus for autosomal dominant hypercholesterolaemia (ADH) and has in recent years become an important target for the development of novel cholesterol lowering therapeutics.  The work carried out for my PhD involved the characterisation of the protein-protein interaction that PCSK9 forms with the LDLR and the investigation of strategies for its pharmacological inhibition.

After completion of my PhD I moved to the University Hospital of North Staffordshire to train as a Clinical Biochemist, a role that as well as providing formalised clinical scientist training has also allowed me to partake in a significant amount of research. Specific areas of interest include the genetics of dietary folic acid utilisation and pregnancy outcome, the use of laboratory investigations in the diagnosis and monitoring of diabetes mellitus and biochemical markers of cardiovascular disease.

ISTM research theme:

Research Interests:

  1. Fetal origins of later onset disease
    Genetic and environmental influences of pregnancy outcome and risk of future disease. As a member of the Fetal Epigenetic Group (FEG), I have investigated the association of polymorphisms in genes involved in the methylation of DNA, through the metabolism and utilisation of dietary folic acid, and short-term measures of pregnancy outcome (e.g. birth weight)

  2. Diabetes
    Work to investigate whether glycated haemoglobin (HbA1c) has a role in the diagnosis of gestational diabetes mellitus (GDM).
    Clinical audit work surrounding the long-term follow-up of women diagnosed with GDM, assessing adherence to NICE guidance 

  3. Cardiovascular disease
    As a member of a European working group for cardiac biomarkers, I am involved in European-wide collaborative studies into the appropriate use and clinical effectiveness of biomarkers (e.g. B-type natriuretic peptide, BNP) in cardiovascular disease. I also have interests in the effective use of cardiac biomarkers in clinical practice at a local level. 

Peer-reviewed publications

Duff, C.J. and Hooper, N.M. (2011). PCSK9: an emerging target for treatment of hypercholesterolemia. Expert Opin Ther Targets 15, 157-168.

Duff, C.J., Scott, M.J., Kirby, I.T., Hutchinson, S.E., Martin, S.L. and Hooper, N.M. (2009). Antibody-mediated disruption of the interaction between PCSK9 and the low-density lipoprotein receptor. Biochem J 419, 577-584.

 

Published abstracts

Duff, C.J., Haworth, K.E., Farrell, W.E., Emes, R.D., Ismail, K.M.K., Carroll, W.D., Borthwick, H.A.,

Yates, A.M., Hubball, E., Rooney, A., Khanam, M., Aggarwal, N., Fryer, A.A. (2013). Genetic polymorphisms in the one-carbon metabolic pathway and their association with birth weight. Ann Clin Biochem 50, Suppl 1, 13-14.

Duff, C.J., Hanna, F.W., Hitchin, S.A., Hodgson, E., Stirling, K. and Fryer, A.A. (2012). An evaluation of laboratory testing in the diagnosis of gestational diabetes mellitus: a potential role for HbA1c? Ann Clin Biochem 49, Suppl 1, 88.

Fryer, A.A., Hitchin, S.A., Duff, C.J., Hodgson, E., Stirling, K. and Hanna, F.W. (2011). Does HbA1c have a role as a diagnostic tool in gestational diabetes mellitus (GDM)? Association of British Clinical Diabetologists Autumn Meeting 2011 Abstracts. Practical Diabetes 29, 124a-124e.

 

Key oral presentations

April 2013            ACB Focus, York, UK. ‘Genetic polymorphisms in the one-carbon metabolic pathway and their association with birth weight’

March 2010         PCSK9 Conference, Nantes, France. ‘The development of small molecules to inhibit PCSK9 function’.

July 2008              Gordon Research Conference: Proprotein Processing, Trafficking and Secretion, New Hampshire, USA. ‘Inhibition of the PCSK9-LDLR interaction: A potential therapeutic strategy’.

 

Professional Memberships: