We offer the following microscopical techniques:

Light microscopy

  • paraffin or resin embedding for light microscopy
  • sectioning (thick or semi-thin)
  • standard anatomical and histochemical staining
  • immunofluorescence or immunoperoxidase staining
  • bright field, phase contrast or Nomarski DIC

Confocal and two-photon imaging

  • imaging of sections, cell cultures or whole mounts
  • immunofluorescence
  • 3-D image stacking and processing
  • in vitro vital dye imaging with time-course analysis

Conventional transmission electron microscopy

  • resin embedding for TEM
  • sectioning (semi-thin or ultrathin )
  • standard ultrastructural staining
  • negative staining
  • quantitative and semi-quantitative immunogold labelling using antibodies
  • stereological analysis

High resolution scanning electron microscopy

  • sample dehydration (critical point drying)
  • sample coating – sputter coating with gold or OTOTO
  • wet sample imaging
  • high resolution imaging ~ 1.5 nm
  • high depth-of-field low power imaging
  • backscattered electron imaging

Conventional and digital imaging

  • all confocal, LM, SEM and TEM images can be acquired digitally
  • conventional photography, photographic development and printing available
  • software available for image quantification and analysis
  • slide maker available for 35 mm slides (from PowerPoint)

Contact us

Central Electron Microscope Unit
School of Life Sciences
Keele University

Professor Dave Furness
Director of the EM Unit
Email :
Tel : 01782 733496

Simon Holborn
Email :
Tel : 01782 733484.