Dr Anja Winter

Title: Lecturer in Biophysics/Biochemistry
Joint Appointee with ILL, Grenoble, France
Phone: (+44) 01782 7 33117
Email: a.winter@keele.ac.uk
Location: Lennard Jones 1.37
Role:
Contacting me: Drop in by my office or fix an appointment by email
Anja Winter

I obtained my MSc in Biochemistry from Freie Universität Berlin, Germany, in 2004 and then went on to do a PhD at the University of Edinburgh. After a brief stay in Brisbane, Australia, I took up a postdoctoral position with Prof. Sir Tom Blundell at the University of Cambridge in 2008. During this post I became interested in fragment-based drug discovery, which is still an emerging field when targeting protein-protein interactions. In 2012 I joined Prof. Richard Bayliss’ lab at the University of Leicester where I worked on separases. In September 2016 I was delighted to start a lectureship position at Keele University which was a joint appointment with ILL in Grenoble, France.  I am married with two children.

I am passionate about conducting science in an area that is helpful to the community – drug research to prevent or cure diseases. My research is focussed on proteins, studying their molecular make-up, investigating how they interact with other proteins and how these interactions can be targeted through drug-discovery. During the past 10 years I developed skills in crystallography, biophysical techniques (CD, fluorescence, DSF, SPR), biochemical and cell-based assays as well as compound design, molecular docking and comparative modelling.

My current focus is on investigating the molecular make-up of prohibitins and to study their interaction with viral envelope proteins. Recent research suggests that several viruses, such as Chikungunya virus, dengue serotype 2 virus and white spot syndrome virus, realise entry into host cells via interaction with prohibitins. But these proteins have also been identified as important players in mitochondrion homeostasis, cell signalling and tumour development.

My post as lecturer is a joint appointment with the Institut Laue-Langevin in Grenoble, France, where I will be utilising their Neutron scattering and X-ray scattering facilities to further my research.

Visit this site for more information:

https://anjawinterresearch.wordpress.com/

Selected publications

  • Winter A., Sigurdardottir, A., DiCara, D., Valenti, G., Blundell, T., Gherardi, E. (2016): Inhibiting Met signalling using a Fragment-based approach, Mol Cancer Ther, 15(1):3-14.
  • Winter, A., Schmid, R., Bayliss, R. (2015): Domain organisation in separases, PLOS Comp Biol, 11(10):e1004548
  • Winter A. et al. (2012): Biophysical and Computational Fragment-based Approaches to Targeting Protein-Protein Interactions: Applications in Structure-guided Drug Discovery, Q Rev Biophys, 45(4):383-426               
  • Winter, A., O. Kamarainen, and A. Hofmann, (2007): Molecular modeling of prohibitin domains. Proteins. 68(1):353-62.   

Full Publication list

  • Bachmann, G., Richards, M., Beuron, F., Winter, A., Morris, E., Bayliss, R. (2016): A closed conformation of the C. elegans separase-securin complex, Open Biology 6(4):160032 
  • Winter A., Sigurdardottir, A., DiCara, D., Valenti, G., Blundell, T., Gherardi, E. (2016): Inhibiting Met signalling using a Fragment-based approach, Mol Cancer Ther, 15(1):3-14.
  • Sigurdardottir, A., Winter, A., Sobkowicz, A., Chirgadze, D., Fragai, M., Ascher, D.B., Gherardi, E. and Blundell, T.L. (2015): Exploring the chemical space of the lysine-binding pocket of hepatocyte growth factor NK1, Chem Science, 6: 6147 – 6157
  • Winter, A., Schmid, R., Bayliss, R. (2015): Domain organisation in separases, PLOS Comp Biol, 11(10):e1004548
  • Karmokar, A. et al. (2014): Assessing molecular mechanisms of curcumin and its in vivo efficacy in targeting colorectal cancer stem-like cells, Mutagenesis 29(6):525-526 
  • Jubb, H., Higueruelo, A., Winter, A., Blundell, T.L. (2012) : Structural biology and drug discovery for protein–protein interactions, TIPS, 33(5):241–248     
  • Winter A. et al. (2012): Biophysical and Computational Fragment-based Approaches to Targeting Protein-Protein Interactions: Applications in Structure-guided Drug Discovery, Q Rev Biophys, 45(4):383-426               
  • Osman A, Wang CK, Winter A, Loukas A, Tribolet L, Gasser RB, Hofmann A. (2012): The crystal structure of an SCP/TAPS protein and its implications for exploring host-pathogen interactions, Biotechnol. Adv., 30(3):652-7                 
  • Blaszczyk, M., Sigurdardottir, A., Winter A., Gherardi, E. and Blundell, T.L. (2011): A structure-guided approach to inhibition of HGF/SF–MET interactions, Acta Cryst. Section A,  67(a1):C348-C349
  • Winter, A. and A. Hofmann, (2008): Towards understanding the roles of prohibitins, multi-functional regulator proteins. Curr Chem Biol. 2(1):11-19   
  • Hu, N.-J., Mohd Yusof, A., Winter, A., Osman, A., Reeve, A.K., Hofmann, A. (2008): The crystal structure of calcium-bound annexin Gh1 from Gossypium hirsutum and its implications for membrane binding mechanisms in plant annexins. J. Biol. Chem. 283(26):18314-22      
  • Winkler K., Winter, A., Rueckert, C.,Uchanska-Ziegler, B.,  Alexiev, U. (2007): Natural MHC class I polymorphism controls the pathway of peptide dissociation from HLA-B27 complexes. Biophys J. 93(8): p. 2743-55
  • Winter, A., O. Kamarainen, and A. Hofmann, (2007): Molecular modeling of prohibitin domains. Proteins. 68(1):353-62.    
  • Winter A., Mohd Yusof, A., Gao, E., Yan, H-L, Hofmann, A. (2006): Biochemical characterization of annexin B1 from Cysticercus cellulosae. FEBS J. 273(14):3238-47.
  • Dabitz, N., Hu NJ,Yusof AM, Tranter N,  Winter A,  Daley M,  Zschörnig O,  Brisson A,  Hofmann A, (2005): Structural determinants for plant annexin-membrane interactions. Biochemistry. 44(49):16292-300.    

Selected Publications

  • McIntyre PJ, Collins PM, Vrzal L, Birchall K, Arnold LH, Mpamhanga C, Coombs PJ, Burgess SG, Richards MW, Winter A, Veverka V, Delft FV, Merritt A, Bayliss R. 2017. Characterization of Three Druggable Hot-Spots in the Aurora-A/TPX2 Interaction Using Biochemical, Biophysical, and Fragment-Based Approaches. ACS Chemical Biology, vol. 12(11), 2906-2914. link> doi> link>
  • DiCara DM, Chirgadze DY, Pope AR, Karatt-Vellatt A, Winter A, Slavny P, van den Heuvel J, Parthiban K, Holland J, Packman LC, Mavria G, Hoffmann J, Birchmeier W, Gherardi E, McCafferty J. 2017. Characterization and structural determination of a new anti-MET function-blocking antibody with binding epitope distinct from the ligand binding domain. Sci Rep, vol. 7(1), 9000. link> doi>
  • Bachmann G, Richards MW, Winter A, Beuron F, Morris E, Bayliss R. 2016. A closed conformation of the Caenorhabditis elegans separase-securin complex. Open Biol, vol. 6(4), 160032. link> doi>
  • Winter A, Sigurdardottir AG, DiCara D, Valenti G, Blundell TL, Gherardi E. 2016. Developing Antagonists for the Met-HGF/SF Protein-Protein Interaction Using a Fragment-Based Approach. Molecular Cancer Therapeutics, vol. 15(1), 3-14. link> doi> link>
  • Winter A, Schmid R, Bayliss R. 2015. Structural Insights into Separase Architecture and Substrate Recognition through Computational Modelling of Caspase-Like and Death Domains. PLoS Computational Biology, vol. 11(10), e1004548. link> doi> link>

Full Publications List show

Journal Articles

  • McIntyre PJ, Collins PM, Vrzal L, Birchall K, Arnold LH, Mpamhanga C, Coombs PJ, Burgess SG, Richards MW, Winter A, Veverka V, Delft FV, Merritt A, Bayliss R. 2017. Characterization of Three Druggable Hot-Spots in the Aurora-A/TPX2 Interaction Using Biochemical, Biophysical, and Fragment-Based Approaches. ACS Chemical Biology, vol. 12(11), 2906-2914. link> doi> link>
  • DiCara DM, Chirgadze DY, Pope AR, Karatt-Vellatt A, Winter A, Slavny P, van den Heuvel J, Parthiban K, Holland J, Packman LC, Mavria G, Hoffmann J, Birchmeier W, Gherardi E, McCafferty J. 2017. Characterization and structural determination of a new anti-MET function-blocking antibody with binding epitope distinct from the ligand binding domain. Sci Rep, vol. 7(1), 9000. link> doi>
  • Bachmann G, Richards MW, Winter A, Beuron F, Morris E, Bayliss R. 2016. A closed conformation of the Caenorhabditis elegans separase-securin complex. Open Biol, vol. 6(4), 160032. link> doi>
  • Winter A, Sigurdardottir AG, DiCara D, Valenti G, Blundell TL, Gherardi E. 2016. Developing Antagonists for the Met-HGF/SF Protein-Protein Interaction Using a Fragment-Based Approach. Molecular Cancer Therapeutics, vol. 15(1), 3-14. link> doi> link>
  • Winter A, Schmid R, Bayliss R. 2015. Structural Insights into Separase Architecture and Substrate Recognition through Computational Modelling of Caspase-Like and Death Domains. PLoS Computational Biology, vol. 11(10), e1004548. link> doi> link>