Professor Ed Mitchell

Mitchell Phone +33(0)4 76 88 26 64

Email : mitchell at esrf.eu

Prof. Mitchell’s research looks at the use of synchrotron X-ray radiation in determining macromolecular structures, with particular interest on several classes of biomolecules:

  • DNA interactions with anti-tumour drugs, with the Institut Laue Langevin (ILL) and Keele, aims to develop new instrumentation and methods to improve the application of the varied array of techniques available on the ESRF-ILL-EMBL international campus and to obtain atomic resolution structures.
  • Iron containing proteins, with the Instituto de Tecnologia Química e Biológica (Lisbon). This work, centred on proteins that are able to safely store iron, looks at the mechanism of the storage and release of iron. These ubiquitous proteins are implicated in the protection, with a mechanism as yet unknown, of DNA under stress conditions.

Prof.  Mitchell is also active in the design and construction of synchrotron X-ray macromolecular crystallography beamlines and is Leader of the Upgrade Programme Project Group laying the foundations for the ESRF Upgrade Programme.

Publications

  • R Leal, S Teixeira, M Blakely, E Mitchell & T Forsyth (2009) A preliminary neutron crystallographic study of an A-DNA crystal, Acta Crys. F, 65, 232-235. [doi:10.1107/S1744309109002668]
  • G Leonard, D Nurizzo, C Dieckmann-Mueller, E Mitchell, J McCarthy, D Flot, I Bento and P Lindley (2008) "Crystallographic Research Developments" in  Astrophysics and Condensed Matter (Horizons in World Physics) Volume 262. Ed. Thomas G. Hardwell.  Nova Science Publishers, Inc. pp 11-69. [ISBN-10: 160456332X]
  • R M F Leal, S C M Teixeira, V Rey, V T Forsyth & E. Mitchell (2008), Absorption correction based on a 3D model reconstruction from visual images, J. Appl. Cryst. 41, 729-737. [doi:10.1107/S0021889808011898]
  • Teixeira, S.C.M., Blakeley, M.P., Leal, R.M., Mitchell, E.P., Forsyth, V.T. (2008), A preliminary neutron crystallographic study of thaumatin, Acta Cryst. F64 (5) [doi:10.1107/S1744309108008294].
  • Kapp, U., Macedo, S., Hall, D.R., Leiros, I.L., McSweeny, S., Mitchell, E. (2008), Structure of Deinococcus radiodurans tunicamycin resistance protein (TmrD), a phosphotransferase, Acta Cryst. F64 [doi: 10.1107/S1744309108011822].
  • J Lescar, J-F Sanchez, A Audfray, J-L Coll, C Breton, E P Mitchell & A Imberty. (2007) Structural basis for recognition of breast and colon cancer epitopes Tn antigen and Forssman disaccharide by Helix pomatia lectinGlycobiology 17, 1077-1083.
  • M G Cuypers, E P Mitchell, C V Romão & S M McSweeney. (2007), The crystal structure of the Dps2 from Deinococcus radiodurans reveals an unusual pore profile with a non-specific metal binding site J. Mol. Biol. 371, 787-799.
  • D Aragão, A M Fialho, A R Marques, E P Mitchell, I Sá-Correia & C Frazão (2007).The complex of Sphingomonas elodea ATCC 31461 glucose-1-phosphate uridylyltransferase with glucose-1-phosphate reveals a novel quaternary structure, unique amongst NDP-sugar pyrophosphorylase members. J. Bact. 189, 4520-4528.
  • C V Romão, E P Mitchell & S McSweeney. (2006). The crystal structure of Deinococcus radiodurans dps protein (dr2263) reveals the presence of a novel metal centre in the N terminus J Biol Inorg Chem 11, 891–902.
  • J F Sanchez, J Lescar, V Chazalet, A Audfray, J Gagnon, R Alvarez, C Breton, A Imberty & E P Mitchell.(2006), Biochemical and structural analysis of Helix pomatia agglutinin. a hexameric lectin with a novel fold J Biol Chem. 281, 20171–20180.
  • C Sabin, E P Mitchell, M Pokorná, C Gautier, J P Utille, M Wimmerova & A Imberty. (2006), Binding of different monosaccharides by lectin pa-2l from Pseudomonas aeruginosa: thermodynamics data correlated with X-ray structures FEBS Lett. 580, 982–987.
  • A Imberty, M Wimmerova, C Sabin & E Mitchell. (2006), Structures and roles of Pseudomonas aeruginosa lectins in Protein-Carbohydrate Interactions in Infectious Disease, C. Bewley (ed.), RSC Biomolecular Sciences Series, Royal Society of Chemistry, pp 30-48.
  • G Cioci, E Mitchell, V Chazalet, H Debray, S Oscarson, M Lahmann, C Gautier, C Breton, S Perez & A Imberty. (2006), - Propeller Crystal Structure of Psathyrella velutina Lectin: An Integrin-like Fungal Protein Interacting with Monosaccharides and Calcium J. Mol. Biol. 357, 1575-1591.
  • D Nurizzo, T Mairs, M Guijarro, V Rey, J Meyer, P Fajardo, J Chavanne, J-C Biasci, S McSweeney, E Mitchell. (2006), The ID23-1 Structural Biology Beamline at the ESRF J. Synchrotron Rad. 13, 227-238.
  • D. Flot, E. J. Gordon, D. R. Hall, G. A. Leonard, A. McCarthy, J. McCarthy, S. McSweeney, E. Mitchell, D. Nurizzo, R. G. B. Ravelli and W. Shepard. (2006), The care and nurture of undulator data sets Acta Cryst. D62, 65-71.
  • A Imberty, E Mitchell & M Wimmerova. (2005), Structural basis for high affinity glycan recognition by bacterial and fungal lectins Current Opinion Structural Biology  15, 525-534.
  • N Kostlanová, E Mitchell, H Lortat-Jacob, S Oscarson, M Lahmann, N Gilboa-Garber, G Chambat, M Wimmerová & A Imberty. (2005), - propeller architecture formed the fucose-binding lectin from Ralstonia solanacearum: a new type of by oligomerisation and interacting with fucose, fucosyllactose and plant xyloglucan J. Biological Chemistry  280, 27839-49.
  • S Arzt, A Beteva, F Cipriani, S Delageniere, F Felisaz, G Förstner, E Gordon, L Launer, B Lavault, G Leonard, T Mairs, A McCarthy, J McCarthy, S McSweeney, J Meyer, E Mitchell, S Monaco, D Nurizzo, R Ravelli, V Rey, W Shepard, D Spruce, O Svensson, P Theveneau. (2005), Automation of macromolecular crystallography beamlines Prog. Biophysics Mol. Biol. 89, 124-152.
  • E Mitchell, C Sabin, L Snajdrova, M Budova, S Perret, C Gautier, C Hofr, N Gilboa-Garber, J Koca, M Wimmerova, & A Imberty. (2005), High affinity fucose binding of Pseudomonas aeruginosa lectin PA-IIL: 1.0 Å resolution crystal structure of the complex combined with thermodynamics and computational chemistry approaches Proteins  58, 735-746.
  • S Macedo, C V Romão, E Mitchell, P M Matias, M Y Liu, A V Xavier, J LeGall, M Teixeira, P Lindley & M A Carrondo. (2003),The nature of the di-iron site in the bacterioferritin from Desulfovibrio desulfuricans. Nature Structural Biology  10, 285-290.
  • E Mitchell, C Houles, D Sudakevitz, M Wimmerova, C Gautier, S Perez, A Wu, N-Gilboa-Garber & A Imberty. (2002), Structural basis for oligosaccharide-mediated adhesion of Psuedomonas aeruginosa in the lungs of cystic fibrosis patients Nature Structural Biology, 9, 918-921.