Mass Spectrometry Facility

The Mass Spectrometry Facility at Keele University is run in collaboration with Guy Hilton Research Centre (GHRC) and the Robert Jones and Agnes Hunt Orthopaedic Hospital (RJAH) in Oswestry. 

We provide proteomics and mass spectrometry services for UK based researchers, engage in research collaborations and implement current technologies in facility services. Click on the tabs below for more information or follow our News section.

Services

Services

We offer comprehensive mass spectrometry and proteomics services, including:

  • Pre-submission and pre-experimental consultation and experimental design

  • Sample preparation: Extraction/Separation through to MS analysis of protein samples

  • Data analysis and Interpretation

  • Training courses

 

Protocols

Protein extraction and preparation

  • Protein Precipitation

  • Protein Purification (TAP, FLAG)

  • Buffer exchange (centrifugal concentrator)

MS Sample Preparation

  • In-gel tryptic digest (Shevchenko et al.)

  • In solution tryptic digest

  • iTRAQ labelling protocol

  • C18 desalting of peptides (ZipTips)

Instruments

Instruments:

Keele MS Facility has both MALDI and ESI platforms for rapid sample characterization and quantification. We possess two state-of-the art mass spectrometers:

  • MALDI 4800 ToF/ToF- Can perform MS and MS/MS of samples in an automated, unbiased manner (Information-Dependent Acquisition)

    • Equipped with a Dionex Ultimate 3000 nano HPLC system and  Probot MALDI spotting system for LC MALDI
    • Resolving power of 18,000 (FWHM) and typical mass accuracy of 50 ppm
    • Typical sensitivity in low fmol range

  • ESI 3200-QTRAP (ABI Sciex) – Tandem quadrupole instrument, typically used for Selected Reaction Monitoring /Multiple Reaction Monitoring experiments for targeted quantification of specific analytes

    • Can also perform product ion analysis of selected precursors (MIDAS)
    • Equipped with online Dionex UltiMate 3000 nanoflowHPLC, typically used for reversed-phase analyte separation

Recommended articles

  • Aebersold R, Mann M.: Mass spectrometry-based proteomics. Nature. 2003 Mar 13;422(6928):198-207.

 

  • Steen H, Mann M.: The ABC's (and XYZ's) of peptide sequencing. Nat Rev Mol Cell Biol. 2004 Sep;5(9):699-711.

 

  • Yates JR, Ruse CI, Nakorchevsky A.: Proteomics by mass spectrometry: approaches, advances, and applications. Annu Rev Biomed Eng. 2009;11:49-79.

 

  • Lange V, Picotti P, Domon B, Aebersold R: Selected reaction monitoring quantitative proteomics: a tutorial. Mol Syst Biol. 2008;4:222. 

Contact details

Please contact Sarah or Elzbieta prior sample preparation and submission

Dr Sarah R Hart

+44 (0)1782 733759

Email: s.r.hart@keele.ac.uk

 

Dr Elzbieta M Piatkowska

Room 214, Huxley Building

Tel: +44 (0)1782 733678

Email: e.m.piatkowska@keele.ac.uk

Location

The Keele MS Facility is located in Huxley Building - building 16 on the Keele Campus Guide and Map‌ (download as pdf).

For directions to Keele University campus please see How to Find Us.