Dr Annette K Shrive

Title: Lecturer
Phone: +44 (0)1782 73419
Email:
Location: Huxley Building : 212a
Role: Senior Tutor
Contacting me: Try my office or e-mail to set up an appointment.
ashrive

I undertook both my BSc (Physics and Maths) and PhD (Protein Crystallography) at Keele University. The latter involved the development of synchrotron x-ray Laue method for use with large macromolecules such as proteins and viruses. I revisited Keele as a postdoctoral worker and was appointed as a Lecturer at Keele University in September 1999. I have been a visiting scientist at Purdue University, USA and LMB Cambridge and I have undertaken postdoctoral work in structural biology at Keele University, University of Edinburgh and Purdue University, USA.

hspd My research interests are in structural biology and in how the structure of biological macromolecules is related to their function. My research is focused on the structure and function of key molecules of the innate immune system. The innate immune response has non-variable components which respond in a fairly general, often rapid manner to threatening microorganisms and molecules of both internal and external origin. A major focus of my research is the definition of the three-dimensional structures of pentraxins and active fragments of collectins. I am part of the Structural Biology Research Group within the Institute for Science and Technology in Medicine (ISTM).

Selected Publications

  • Pionnier N, Falco A, Miest J, Frost P, Irnazarow I, Shrive A, Hoole D. 2013. Dietary β-glucan stimulate complement and C-reactive protein acute phase responses in common carp (Cyprinus carpio) during an Aeromonas salmonicida infection. Fish Shellfish Immunol, vol. 34(3), 819-831. link> doi>
  • Mold M, Shrive AK, Exley C. 2012. Serum amyloid P component accelerates the formation and enhances the stability of amyloid fibrils in a physiologically significant under-saturated solution of amyloid-β42. J Alzheimers Dis, vol. 29(4), 875-881. link> doi>
  • Shrive AK, Martin C, Burns I, Paterson JM, Martin JD, Townsend JP, Waters P, Clark HW, Kishore U, Reid KB, Greenhough TJ. 2009. Structural characterisation of ligand-binding determinants in human lung surfactant protein D: influence of Asp325. J Mol Biol, vol. 394(4), 776-788. link> doi>
  • Shrive AK, Burns I, Chou HT, Stahlberg H, Armstrong PB, Greenhough TJ. 2009. Crystal structures of Limulus SAP-like pentraxin reveal two molecular aggregations. J Mol Biol, vol. 386(5), 1240-1254. link> doi>
  • MacCarthy EM, Burns I, Irnazarow I, Polwart A, Greenhough TJ, Shrive AK, Hoole D. 2008. Serum CRP-like protein profile in common carp Cyprinus carpio challenged with Aeromonas hydrophila and Escherichia coli lipopolysaccharide. Dev Comp Immunol, vol. 32(11), 1281-1289. link> doi>

Full Publications List show

Journal Articles

  • Pionnier N, Falco A, Miest J, Frost P, Irnazarow I, Shrive A, Hoole D. 2013. Dietary β-glucan stimulate complement and C-reactive protein acute phase responses in common carp (Cyprinus carpio) during an Aeromonas salmonicida infection. Fish Shellfish Immunol, vol. 34(3), 819-831. link> doi>
  • Mold M, Shrive AK, Exley C. 2012. Serum amyloid P component accelerates the formation and enhances the stability of amyloid fibrils in a physiologically significant under-saturated solution of amyloid-β42. J Alzheimers Dis, vol. 29(4), 875-881. link> doi>
  • Shrive AK, Martin C, Burns I, Paterson JM, Martin JD, Townsend JP, Waters P, Clark HW, Kishore U, Reid KB, Greenhough TJ. 2009. Structural characterisation of ligand-binding determinants in human lung surfactant protein D: influence of Asp325. J Mol Biol, vol. 394(4), 776-788. link> doi>
  • Shrive AK, Burns I, Chou HT, Stahlberg H, Armstrong PB, Greenhough TJ. 2009. Crystal structures of Limulus SAP-like pentraxin reveal two molecular aggregations. J Mol Biol, vol. 386(5), 1240-1254. link> doi>
  • MacCarthy EM, Burns I, Irnazarow I, Polwart A, Greenhough TJ, Shrive AK, Hoole D. 2008. Serum CRP-like protein profile in common carp Cyprinus carpio challenged with Aeromonas hydrophila and Escherichia coli lipopolysaccharide. Dev Comp Immunol, vol. 32(11), 1281-1289. link> doi>
  • Kishore U, Greenhough TJ, Waters P, Shrive AK, Ghai R, Kamran MF, Bernal AL, Reid KB, Madan T, Chakraborty T. 2006. Surfactant proteins SP-A and SP-D: structure, function and receptors. Mol Immunol, vol. 43(9), 1293-1315. link> doi>
  • Kishore U, Ghai R, Greenhough TJ, Shrive AK, Bonifati DM, Gadjeva MG, Waters P, Kojouharova MS, Chakraborty T, Agrawal A. 2005. Structural and functional anatomy of the globular domain of complement protein C1q (vol 95, pg 113, 2004). IMMUNOLOGY LETTERS, vol. 101(1), II. link> doi>
  • GREENHOUGH TJ, Gaboriaud C, Kishore U, Waters P. 2004. C1q and tumor necrosis factor superfamily: modularity and versatility. Trends in Immunology, vol. 25(10), 551-561. doi>
  • Kishore U, Ghai R, Greenhough TJ, Shrive AK, Bonifati DM, Gadjeva MG, Waters P, Kojouharova MS, Chakraborty T, Agrawal A. 2004. Structural and functional anatomy of the globular domain of complement protein Clq. Immunology Letters, vol. 95(2), 113-128. doi>
  • Cartwright JR, Tharia HA, Burns I, Shrive AK, Hoole D, Greenhough TJ. 2004. Isolation and characterisation of pentraxin-like serum proteins from the common carp Cyprinus carpio. Dev Comp Immunol, vol. 28(2), 113-125. link> doi>
  • Shrive AK, Tharia HA, Strong P, Kishore U, Burns I, Rizkallah PJ, Reid KB, Greenhough TJ. 2003. High-resolution structural insights into ligand binding and immune cell recognition by human lung surfactant protein D. J Mol Biol, vol. 331(2), 509-523. link> doi>
  • Lee WH, Perles LA, Nagem RAP, Shrive AK, Hawkins A, Sawyer L, Polikarpov I. 2003. Comparison of different crystal forms of 3-dehydroquinase from \fISalmonella typhi\fP and its implication for the enzyme activity. Acta Crystallographica, Section D. doi>
  • Hoole D, Lewis JW, Schuwerack PM, Chakravarthy C, Shrive AK, Greenhough TJ, Cartwright JR. 2003. Inflammatory interactions in fish exposed to pollutants and parasites: a role for apoptosis and C reactive protein. Parasitology, vol. 126 Suppl, S71-S85. link> doi>
  • Ramadan MA, Shrive AK, Holden D, Myles DA, Volanakis JE, DeLucas LJ, Greenhough TJ. 2002. The three-dimensional structure of calcium-depleted human C-reactive protein from perfectly twinned crystals. Acta Crystallogr D Biol Crystallogr, vol. 58(Pt 6 Pt 2), 992-1001. link> doi> full text>
  • Tharia HA, Shrive AK, Mills JD, Arme C, Williams GT, Greenhough TJ. 2002. Complete cDNA sequence of SAP-like pentraxin from Limulus polyphemus: implications for pentraxin evolution. J Mol Biol, vol. 316(3), 583-597. link> doi>
  • Agrawal A, Shrive AK, Greenhough TJ, Volanakis JE. 2001. Topology and structure of the C1q-binding site on C-reactive protein. J Immunol, vol. 166(6), 3998-4004. link>
  • Shrive AK, Metcalfe AM, Cartwright JR, Greenhough TJ. 1999. C-reactive protein and SAP-like pentraxin are both present in Limulus polyphemus haemolymph: crystal structure of Limulus SAP. J Mol Biol, vol. 290(5), 997-1008. link> doi>
  • Gourley DG, Shrive AK, Polikarpov I, Krell T, Coggins JR, Hawkins AR, Isaacs NW, Sawyer L. 1999. The two types of 3-dehydroquinase have distinct structures but catalyze the same overall reaction. Nat Struct Biol, vol. 6(6), 521-525. link> doi>
  • Leech AP, Boetzel R, McDonald C, Shrive AK, Moore GR, Coggins JR, Sawyer L, Kleanthous C. 1998. Re-evaluating the role of His-143 in the mechanism of type I dehydroquinase from Escherichia coli using two-dimensional 1H,13C NMR. J Biol Chem, vol. 273(16), 9602-9607. link> doi>
  • Shrive AK, Holden D, Myles DA, Greenhough TJ. 1996. Structure solution of C-reactive proteins: molecular replacement with a twist. Acta Crystallogr D Biol Crystallogr, vol. 52(Pt 6), 1049-1057. link> doi>
  • Shrive AK, Cheetham GM, Holden D, Myles DA, Turnell WG, Volanakis JE, Pepys MB, Bloomer AC, Greenhough TJ. 1996. Three dimensional structure of human C-reactive protein. Nat Struct Biol, vol. 3(4), 346-354. link> doi>
  • SHRIVE A and GREENHOUGH T. 1994. LAUE IMAGE-ANALYSIS .3. THE COLLECTION AND ANALYSIS OF DELIBERATELY STREAKED LAUE EXPOSURES. JOURNAL OF APPLIED CRYSTALLOGRAPHY, vol. 27, 532-542. link> doi>
  • Hubbard SR, Greenall RJ, Shrive AK, Forsyth VT, Mahendrasingam A. 1994. Structure factor calculations for a side-by-side model of B-DNA. Int J Biol Macromol, vol. 16(4), 195-205. link> doi>
  • GREENHOUGH T and SHRIVE A. 1994. LAUE IMAGE-ANALYSIS .2. VARIABLE RADIAL ELLIPTIC MASKING AND ITS APPLICATION IN STUDIES INVOLVING INHERENTLY STREAKED LAUE EXPOSURES. JOURNAL OF APPLIED CRYSTALLOGRAPHY, vol. 27, 111-121. link> doi>
  • Campbell JW, Clifton IJ, Greenhough TJ, Hajdu J, Harrison SC, Liddington RC, Shrive AK. 1990. Calcium binding sites in tomato bushy stunt virus visualized by Laue crystallography. J Mol Biol, vol. 214(3), 627-632. link> doi>
  • SHRIVE A, CLIFTON I, HAJDU J, GREENHOUGH T. 1990. LAUE FILM INTEGRATION AND DECONVOLUTION OF SPATIALLY OVERLAPPING REFLECTIONS. JOURNAL OF APPLIED CRYSTALLOGRAPHY, vol. 23, 169-174. link> doi>

Other

  • Cartwright JR, MacCarthy E, Greenhough TJ, Shrive A, Wiegertjes GF, Hoole D. 2003. Pentraxin-like serum proteins from common carp \fI(Cyprinus carpio)\fP. Proceedings of the 11th International Conference of the European Association of Fish Pathologists.
  • Cartwright JR, Greenhough TJ, Shrive AK, Hoole D. 2001. Isolation of C-reactive protein from the common carp \fI(Cyprinius carpio)\fP. Proceedings of the International Conference of the European Association of Fish Pathologists.
  • Hajdu J, Greenhough TJ, Clifton IJ, Campbell JW, Shrive AK, Harrison SC, Liddington RC. 1989. Laue crystallography: application to virus crystals. Basic Life Sci (vol. 51, pp. 331-339). link>
  • Shrive AK, Shaw AJ, Ahmed A, Paterson JM, Smallcombe C, Reid KBM, Deadman ME, Hood DW, Holmskov U, Clark HW, Madsen J, Greenhough TJ. Structural basis of ligand and pathogen recognition by the collectins. Acta Crystallographica Section A: Foundations of Crystallography (vol. A67, p. C472).
  • Greenhough TJ, Mold M, Moran J, Burns I, Paterson JM, Bailey SJ, Bailey J, Armstrong PB, Shrive AK. Structure, function and evolution of the serum pentraxins. Acta Crystallographica Section A: Foundations of Crystallography (vol. A67, p. C546).

  • LSC10030 Case Studies in Medicine

  • LSC20015 Molecular, Cellular & Structural Immunology (Module manager)

  • LSC30007 Biology dissertation

  • LSC30008 /12 Experimental Research project

  • LSC30014 Biochemistry Research project (Non-experimental)

  • LSC30015 Biology of Disease (Module manager)

  • LSC30016 Structural Biology & Macromolecular Function

  • MTE40018 Physiology and Anatomy