ISTM

I came to Keele with an EPSRC International Life Science Interface Fellowship, worth £336,000: “Development of Advanced Mass Spectrometric Approaches for the Analysis of the Proteome of Serum from Women with Pre-Eclampsia”.  This Fellowship has enabled me to work with the Zubarev lab in Sweden and the Burlingame lab at UCSF on electron-mediated peptide dissociation methods.

My post-doctoral Research work from 2003-07 was in the Michael Barber Centre for Mass Spectrometry at Manchester University, supervised by Prof Simon Gaskell, on a project entitled “Investigation of the African trypanosome flagellar proteome”.

Prior to that, my PhD in Biochemistry & Molecular Biology was at the Ludwig Institute for Cancer Research, University College London. Supervised by Prof Michael Waterfield, the thesis title was “The Analysis of Protein Phosphorylation Sites using Affinity Chromatography and Mass Spectrometry”.

ISTM research theme: Clinical & Diagnostic Science

My research interests are primarily in the field of biological mass spectrometry and its application to the improved understanding of biological and clinically-significant samples.

I have interests in the development of novel methods which improve our ability to process, analyse and derive valuable quantitative and qualitative information about the components of interest within complex biological samples. I have worked closely with industrial and academic partners to implement new methods in proteomics (Hart et al., J Am Soc Mass Spectrom., 2009).

I am interested in the gas-phase behaviour of large polypeptide ions (Lau et al., Rapid Commun. Mass Spectrom., 2009), and in differences between the behaviour of product ions generated using different methods for fragmenting these polypeptides (Hart et al., Methods Mol. Biol., in Press 2010).

Recent peer-reviewed publications:

1) Lau KW, Hart SR, Lynch JA, Wong SCC, Hubbard SJ, Gaskell SJ, “Observations on the Detection of b- and y-Type Ions in the Collisionally Activated Decomposition Spectra of Protonated Peptides”, Rapid Communications in Mass Spectrometry, 2009, 23, 10 1508-14. doi:10.1002/rcm.4032

2) Hart SR, Lau KW, Hao Z, Broadhead R, Portman N, Huhmer A, Gull K, McKean PG, Hubbard SJ, Gaskell SJ, “Analysis of the Trypanosome Flagellar Proteome using a Combined Electron Transfer Collisionally Activated Dissociation Strategy”, J. Am. Soc. Mass Spectrom., 2009, 20, 2 167-175. doi:10.1016/j.jasms.2008.08.014

3) Siepen JA, Swainston N, Jones AR, Hart SR, Hermjakob H, Jones P, Hubbard SJ, “An informatic pipeline for the data capture and submission of quantitative proteomic data using iTRAQTM”, Proteome Science, 2007, 5, 4. doi: 10.1186/1477-5956-5-4.

4) Castrillo JI, Zeef LA, Hoyle DC, Zhang N, Hayes A, Gardner DCJ, Cornell MJ, Petty J, Hakes L, Wardleworth L, Rash B, Brown M, Dunn WB, Broadhurst D, O'Donoghue K, Hester SS, Dunkley TP, Hart SR, Swainston, N, Li P, Gaskell SJ, Paton NW, Lilley KS, Kell DB, Oliver SG, “Growth control of the eukaryote cell: A systems biology study in yeast”, Journal of Biology, 2007, 6, 4. doi:10.1186/jbiol54

5) Myers JE, Hart SR, Armstrong S, Mires G, Beynon R, Gaskell SJ, Baker P, “There is no single circulating factor in pre-eclampsia (toxaemia)”, American Journal of Obstetrics and Gynaecology, 2007, 196, 266.e1-6. doi:10.1016/j.ajog.2006.10.875

6) Broadhead R, Dawe HR, Farr H, Griffiths S, Hart SR, Portman N, Shaw MK, Ginger ML, Gaskell SJ, McKean PG, Gull, K “Flagellar motility is required for the viability of the bloodstream trypanosome”, Nature, 2006, 440. 224-227. doi:10.1038/nature04541

7) Barnouin KN, Hart SR, Thompson AJ., Okuyama M, Waterfield MD, Cramer R, “Enhanced phosphopeptide isolation by Fe(III)-immobilised metal-ion affinity chromatography using 1,1,1,3,3,3-Hexafluoroisopropanol”, Proteomics 2005, 5: 4376-88. doi:10.1002/pmic.200401323

Book chapters

“Distributions of ion series in ETD and CAD spectra: making a comparison”, SR Hart, KW Lau and SJ Hubbard, Methods in Molecular Biology. In Press 2010.

“A protocol for top down proteomics using HPLC and ETD/PTR-MS”, SR Hart, in Methods in Molecular Biology, P.R. Cutillas Ed., In Press 2010.

“Methods of proteome analysis: challenges and opportunities”, SR Hart and SJ Gaskell, in Practical Systems Biology, 2008. A Hetherington; C Grierson Eds., Taylor & Francis Ltd, UK. ISBN: 9780415407809.

At Keele I shall act as a Problem-Based Learning tutor from Autumn 2010 onwards.

As a Post-Doc and then Research Fellow at the Michael Barber Centre for Mass Spectrometry, Manchester, I was closely involved in the management and delivery of the successful CPD course ‘Mass Spectrometry for Protein & Peptide Scientists’. I taught on the MRes in Post-Genome Science, and currently remain a module co-ordinator for programs on Manchester’s Doctoral Training Centre in Systems Biology and MRes in Translational Medicine.

Memberships of professional organisations and external roles:

I was awarded Membership of the Royal Society for Chemistry in 2006.

I am a co-opted member of the British Mass Spectrometry Society committee and am actively engaged with the Education sub-committee.

I am a member of the American Society for Mass Spectrometry and the Biochemical Society.

I am also a registered STEM Ambassador, and regularly take part in public events promoting science and school events to help engage the next generation of young scientists.