Dr. Susana C. M. Teixeira

Title: Joint Institut Laue Langevin/Keele University Lecturer (Chemical/Physical Sciences)
Phone: (+44) 01782 7 34442
Email:
Location: Lennard-Jones 1.37
Role:
Contacting me:
Teixeira_Susana

I did my undergraduate studies in Portugal when I developed an interest in Applied Chemistry, within a 5-year Chemical Engineering degree. During this time I had the opportunity to do a number of courses abroad, but it was an Erasmus program in Reading University that brought me into Crystallography. I went back to Portugal determined to find out more about the world of crystals. After my graduation I worked in Protein Crystallography laboratories in Portugal for nearly two years, before I started my PhD at Reading University. Here I worked mostly with biological crystals. I then moved on to a 5-year Postdoctoral fellowship at Keele University, based in Grenoble where there are facilities for Deuteration of biological samples for neutron crystallography studies among many other structural tools.

In 2007 I became a Lecturer at Keele University, where I now add the privilege of working on a campus where both neutron and x-ray world leading facilities are, to the opportunity to teach Structural Biology. It is a challenging job but I hope to learn a lot with it and share my enthusiasm with Crystallography as a unique tool to look into the atomic world.

trixiera image

At Grenoble I am co-responsible for a low resolution neutron diffractometer (D16) and a Laue diffractometer (LADI-III).  The two beamlines cover a wide range of structural information in crystallography, from crystal structures of complex systems such as membrane proteins or viruses to smaller proteins and oligonucleotides. D16 also finds applications in crystallogenesis, while LADI-III allows for the determination of accurate positions of hydrogen/deuterium atoms. This detailed level of structural information is essential in grasping enzymatic mechanisms and molecular recognition.

Deuterium labelling of the sample plays an essential role for the use of contrast variation techniques, allowing for better signal-to-noise ratios (crystals as small as 0.01mm3 can be used) and for different components of an assembly to be highlighted, for example in a protein/DNA complex. At the ILL/EMBL Deuteration Laboratory, in Grenoble, we focus on Deuteration techniques for biological samples. The “D-Lab” is where I currently prepare crystal samples to study complexes of DNA with potential anti-cancer drugs, as well as for neutron studies of food-related systems such as thaumatin (see image).

See also the Macromolecular Structure Research Group and my page at Institut Laue-Langevin.

Selected Publications

  • Haupt M, Blakeley MP, Teixeira SC, Mason SA, Mitchell EP, Cooper JB, Forsyth VT. 2011. Preliminary neutron crystallographic study of human transthyretin. Acta Crystallogr Sect F Struct Biol Cryst Commun, vol. 67(Pt 11), 1428-1431. link> doi>
  • Tiggelaar SM, Mossou E, Callow P, Callow S, Teixeira SC, Mitchell EP, Mitraki A, Forsyth VT. 2011. Neutron fibre diffraction studies of amyloid using H2O/D2O isotopic replacement. Acta Crystallogr Sect F Struct Biol Cryst Commun, vol. 67(Pt 3), 332-335. link> doi>
  • Leal RM, Callow S, Callow P, Blakeley MP, Cardin CJ, Denny WA, Teixeira SC, Mitchell EP, Forsyth VT. 2010. Combined neutron and X-ray diffraction studies of DNA in crystals and solutions. Acta Crystallogr D Biol Crystallogr, vol. 66(Pt 11), 1244-1248. link> doi>
  • Artero JB, Teixeira SC, Mitchell EP, Kron MA, Forsyth VT, Haertlein M. 2010. Crystallization and preliminary X-ray diffraction analysis of human cytosolic seryl-tRNA synthetase. Acta Crystallogr Sect F Struct Biol Cryst Commun, vol. 66(Pt 11), 1521-1524. link> doi>
  • Blakeley MP, Teixeira SC, Petit-Haertlein I, Hazemann I, Mitschler A, Haertlein M, Howard E, Podjarny AD. 2010. Neutron macromolecular crystallography with LADI-III. Acta Crystallogr D Biol Crystallogr, vol. 66(Pt 11), 1198-1205. link> doi>

Full Publications List show

Journal Articles

  • Haupt M, Blakeley MP, Teixeira SC, Mason SA, Mitchell EP, Cooper JB, Forsyth VT. 2011. Preliminary neutron crystallographic study of human transthyretin. Acta Crystallogr Sect F Struct Biol Cryst Commun, vol. 67(Pt 11), 1428-1431. link> doi>
  • Tiggelaar SM, Mossou E, Callow P, Callow S, Teixeira SC, Mitchell EP, Mitraki A, Forsyth VT. 2011. Neutron fibre diffraction studies of amyloid using H2O/D2O isotopic replacement. Acta Crystallogr Sect F Struct Biol Cryst Commun, vol. 67(Pt 3), 332-335. link> doi>
  • Leal RM, Callow S, Callow P, Blakeley MP, Cardin CJ, Denny WA, Teixeira SC, Mitchell EP, Forsyth VT. 2010. Combined neutron and X-ray diffraction studies of DNA in crystals and solutions. Acta Crystallogr D Biol Crystallogr, vol. 66(Pt 11), 1244-1248. link> doi>
  • Artero JB, Teixeira SC, Mitchell EP, Kron MA, Forsyth VT, Haertlein M. 2010. Crystallization and preliminary X-ray diffraction analysis of human cytosolic seryl-tRNA synthetase. Acta Crystallogr Sect F Struct Biol Cryst Commun, vol. 66(Pt 11), 1521-1524. link> doi>
  • Blakeley MP, Teixeira SC, Petit-Haertlein I, Hazemann I, Mitschler A, Haertlein M, Howard E, Podjarny AD. 2010. Neutron macromolecular crystallography with LADI-III. Acta Crystallogr D Biol Crystallogr, vol. 66(Pt 11), 1198-1205. link> doi>
  • Teixeira SC, Blakeley MP, Leal RM, Gillespie SM, Mitchell EP, Forsyth VT. 2010. Sweet neutron crystallography. Acta Crystallogr D Biol Crystallogr, vol. 66(Pt 11), 1139-1143. link> doi>
  • Leal RM, Teixeira SC, Blakeley MP, Mitchell EP, Forsyth VT. 2009. A preliminary neutron crystallographic study of an A-DNA crystal. Acta Crystallogr Sect F Struct Biol Cryst Commun, vol. 65(Pt 3), 232-235. link> doi>
  • Leal RMF, Teixeira SCM, Rey V, Forsyth VT, Mitchell EP. 2008. Absorption correction based on a three-dimensional model reconstruction from visual images. JOURNAL OF APPLIED CRYSTALLOGRAPHY, vol. 41, 729-737. link> doi>
  • Teixeira SC, Blakeley MP, Leal RM, Mitchell EP, Forsyth VT. 2008. A preliminary neutron crystallographic study of thaumatin. Acta Crystallogr Sect F Struct Biol Cryst Commun, vol. 64(Pt 5), 378-381. link> doi>
  • Papanikolopoulou K, Teixeira S, Belrhali H, Forsyth VT, Mitraki A, van Raaij MJ. 2004. Adenovirus fibre shaft sequences fold into the native triple beta-spiral fold when N-terminally fused to the bacteriophage T4 fibritin foldon trimerisation motif. J Mol Biol, vol. 342(1), 219-227. link> doi>
  • Thorpe JH, Gale BC, Teixeira SC, Cardin CJ. 2003. Conformational and hydration effects of site-selective sodium, calcium and strontium ion binding to the DNA Holliday junction structure d(TCGGTACCGA)(4). J Mol Biol, vol. 327(1), 97-109. link> doi>
  • Thorpe JH, Teixeira SC, Gale BC, Cardin CJ. 2003. Crystal structure of the complementary quadruplex formed by d(GCATGCT) at atomic resolution. Nucleic Acids Res, vol. 31(3), 844-849. link> doi>
  • TEIXEIRA SCM, Powell H, Thorpe JH, Todd AK. 2002. Structural characterisation of bisintercalation in higher-order DNA at a junction-like quadruplex. Journal of Molecular Biology, vol. 323(2), 167-171. doi>
  • TEIXEIRA SCM, Dias JM, Macieira S, Raaijmakers H. 2002. Gene sequence and the 1.8Å Crystal Structure of the Tungsten-Containing Formate Dehydrogenase from Desulfovibrio gigas. Structure, vol. 10(9), 1261-1272. doi>
  • Thorpe JH, Teixeira SC, Gale BC, Cardin CJ. 2002. Structural characterization of a new crystal form of the four-way Holliday junction formed by the DNA sequence d(CCGGTACCGG)2: sequence versus lattice?. Acta Crystallogr D Biol Crystallogr, vol. 58(Pt 3), 567-569. link> doi>
  • Lo Leggio L, Kalogiannis S, Eckert K, Teixeira SC, Bhat MK, Andrei C, Pickersgill RW, Larsen S. 2001. Substrate specificity and subsite mobility in T. aurantiacus xylanase 10A. FEBS Lett, vol. 509(2), 303-308. link> doi>
  • TEIXEIRA SCM, Cardin C, Lo Leggio L, Pickersgill R. 2001. Anisotropic refinement of the structure of Thermoascus aurantiacus xylanase I. Acta Crystallographica D: Biological Crystallography, vol. 57(3), 385-392. doi>
  • Teixeira S, Dias JM, Carvalho AL, Bourenkov G, Bartunik H, Almendra MJ, Moura I, Moura JJG, Romao MJ. 1999. Crystallographic studies on a tungsten-containning formate dehydrogenase from Desulfovibrio gigas. JOURNAL OF INORGANIC BIOCHEMISTRY, vol. 74(1-4), 89. link>
  • Benet S, Cardin CJ, Cardin DJ, Constantine SP, Heath P, Rashid H, Teixeira S, Thorpe JH, Todd AK. 1999. Syntheses and crystal structures of heteroleptic stannylenes and germylenes. ORGANOMETALLICS, vol. 18(3), 389-398. link> doi>
  • Hockemeyer J, Valentin B, Castel A, Riviere P, Satge J, Cardin CJ, Teixeira S. 1997. Synthesis and structure of germanium- and tin-bridged macrocycles: Germa- and stanna-calixarenes. MAIN GROUP METAL CHEMISTRY, vol. 20(12), 775-781. link>
  • MalamidouXenikaki E, Stampelos XN, CoutouliArgyropoulou E, Cardin CJ, Teixeira S, Kavounis CA. 1997. Synthesis of indole substituted dihydro- and tetrahydro-isoxazoles via 1,3-dipolar cycloadditions of C-(1-methylindol-2-yl)-N-methylnitrone with unsaturated carbonyl derivatives and a study of their acid-induced intramolecular cyclizations. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 949-957. link> doi>
  • Kladova A, Gavel O, Mukhopaadhyay A, Boer D, Teixeira S, Shnyrov V, Moura I, Moura J, Romao M, Trincao J, Bursakov S. Cobalt-, zinc- and iron-bound forms of adenylate kinase (AK) from the sulfate-reducing bacterium Desulfovibrio gigas: purification, crystallization and preliminary X-ray diffraction analysis. doi>
  • Seyeradabi A, To TT, Teixeira S, Hussain S, Fries M, Madsen R, Clausen M, Brocklehurst K, Ali S, Pickersgill R. Structural Insights into Substrate Specificity and the anti β-Elimination Mechanism of Pectate Lyase. Biochemistry, 539-546. doi>

Other

  • Teixeira SCM, Zaccai G, Ankner J, Bellissent-Funel MC, Bewley R, Blakeley MP, Callow P, Coates L, Dahint R, Dalgliesh R. 2008. New sources and instrumentation for neutrons in biology. CHEMICAL PHYSICS (vol. 345, pp. 133-151). ELSEVIER SCIENCE BV. doi>
  • Miles S, Callow P, Teixeira S, Gan Y, Denny W, Cardin C, Forsyth T. 2006. Structural studies on acridine derivatives binding to telomeric DNA. PHYSICA B-CONDENSED MATTER (vols. 385-86, pp. 845-847). link> doi>
  • CHE-20006 : Biophysical Chemistry
  • CHE-30031 : Medicinal and Biomolecular Chemistry
  • MSci in Scientific Research Training (Placement supervisor at the I.L.L.)
  • Hercules course - Neutron crystallography tutor, Grenoble, France
  • M2 Nucleic Acids Course - invited lecturer in Nucleic Acid Structures, Joseph Fourier University, Grenoble